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Sequence-Based Classification and Identification

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Introduction to Bioinformatics in Microbiology

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Abstract

You will be introduced to the classification of prokaryotes from species to the higher levels of class and phyla. You will learn that the 16S rRNA gene sequence comparison including phylogenetic analysis provides the main information for classification. DNA–DNA hybridization predicted in silico from whole genomic sequences is mainly used for classification of species. Now, whole genomic sequences (WGS) are increasingly used for classification and identification. In the activity, you will learn to identify an isolate by 16S rRNA sequence in the EzBioCloud server and by whole genomic sequencing in Type Strain Genome Server (TYGS).

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Christensen, H., Olsen, J.E. (2023). Sequence-Based Classification and Identification. In: Christensen, H. (eds) Introduction to Bioinformatics in Microbiology. Learning Materials in Biosciences. Springer, Cham. https://doi.org/10.1007/978-3-031-45293-2_7

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