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Non-Neoplastic Cytology
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Abstract

The clinical use of immunohistochemical staining is helpful to characterize the lesional cells and determine the site of origin. This chapter summarizes the staining pattern and expression of immunohistochemical stains in normal or benign tissue cells. Some commonly encountered infections and crystals are also described in table form.

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References

  1. Ivanov S, Liao SY, Ivanova A, et al. Expression of hypoxia-inducible cell-surface transmembrane carbonic anhydrases in human cancer. Am J Pathol. 2001;158(3):905–19.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Luong-Player A, Liu H, Wang HL, Lin F. Immunohistochemical reevaluation of carbonic anhydrase IX (CA IX) expression in tumors and normal tissues. Am J Clin Pathol. 2014;141(2):219–25.

    Article  PubMed  Google Scholar 

  3. Lloyd JM, Owens SR. CD10 immunohistochemistry stains enteric mucosa, but negative staining is unreliable in the setting of active enteritis. Mod Pathol. 2011;24(12):1627–32.

    Article  PubMed  Google Scholar 

  4. McCluggage WG, Sumathi VP, Maxwell P. CD10 is a sensitive and diagnostically useful immunohistochemical marker of normal endometrial stroma and of endometrial stromal neoplasms. Histopathology. 2001;39(3):273–8.

    Article  CAS  PubMed  Google Scholar 

  5. Shousha S, Gadir F, Peston D, Bansi D, Thillainaygam AV, Murray-Lyon IM. CD10 immunostaining of bile canaliculi in liver biopsies: change of staining pattern with the development of cirrhosis. Histopathology. 2004;45(4):335–42.

    Article  CAS  PubMed  Google Scholar 

  6. Craig CE, Quaglia A, Dhillon AP. Extramedullary haematopoiesis in massive hepatic necrosis. Histopathology. 2004;45(5):518–25.

    Article  CAS  PubMed  Google Scholar 

  7. Trovato M, Grosso M, Vitarelli E, Le Donne M, Barresi V, Trimarchi F, Barresi G. Immunoexpression of CD30 and CD30 ligand in deciduas from spontaneous abortions. Eur J Histochem. 2005;49(3):285–90.

    Article  CAS  PubMed  Google Scholar 

  8. Choi G, Roh J, Park CS. CD99 is strongly expressed in basal cells of the normal adult epidermis and some subpopulations of appendages: comparison with developing fetal skin. J Pathol Transl Med. 2016;50(5):361–8.

    Article  PubMed  PubMed Central  Google Scholar 

  9. Nguyen TT, Schwartz EJ, West RB, Warnke RA, Arber DA, Natkunam Y. Expression of CD163 (hemoglobin scavenger receptor) in normal tissues, lymphomas, carcinomas, and sarcomas is largely restricted to the monocyte/macrophage lineage. Am J Surg Pathol. 2005;29(5):617–24.

    Article  PubMed  Google Scholar 

  10. Abdulla Z, Turley H, Gatter K, Pezzella F. Immunohistological recognition of cyclin D1 expression by non-lymphoid cells among lymphoid neoplastic cells. APMIS. 2014;122(3):183–91.

    Article  CAS  PubMed  Google Scholar 

  11. Truong LD, Rangdaeng S, Cagle P, Ro JY, Hawkins H, Font RL. The diagnostic utility of desmin. A study of 584 cases and review of the literature. Am J Clin Pathol. 1990;93(3):305–14.

    Article  CAS  PubMed  Google Scholar 

  12. Hasteh F, Lin GY, Weidner N, Michael CW. The use of immunohistochemistry to distinguish reactive mesothelial cells from malignant mesothelioma in cytologic effusions. Cancer Cytopathol. 2010;118(2):90–6.

    Article  PubMed  Google Scholar 

  13. Chênevert J, Duvvuri U, Chiosea S, Dacic S, Cieply K, Kim J, Shiwarski D, Seethala RR. DOG1: a novel marker of salivary acinar and intercalated duct differentiation. Mod Pathol. 2012;25(7):919–29.

    Article  PubMed  Google Scholar 

  14. Espinosa I, Lee CH, Kim MK, Rouse BT, Subramanian S, Montgomery K, Varma S, Corless CL, Heinrich MC, Smith KS, Wang Z, Rubin B, Nielsen TO, Seitz RS, Ross DT, West RB, Cleary ML, van de Rijn M. A novel monoclonal antibody against DOG1 is a sensitive and specific marker for gastrointestinal stromal tumors. Am J Surg Pathol. 2008;32(2):210–8.

    Article  PubMed  Google Scholar 

  15. Miettinen M, McCue PA, Sarlomo-Rikala M, Rys J, Czapiewski P, Wazny K, Langfort R, Waloszczyk P, Biernat W, Lasota J, Wang Z. GATA3: a multispecific but potentially useful marker in surgical pathology: a systematic analysis of 2500 epithelial and nonepithelial tumors. Am J Surg Pathol. 2014;38(1):13–22.

    Article  PubMed  PubMed Central  Google Scholar 

  16. Stead RH, Qizilbash AH, Kontozoglou T, Daya AD, Riddell RH. An immunohistochemical study of pleomorphic adenomas of the salivary gland: glial fibrillary acidic protein-like immunoreactivity identifies a major myoepithelial component. Hum Pathol. 1988;19(1):32–40.

    Article  CAS  PubMed  Google Scholar 

  17. Shafizadeh N, Ferrell LD, Kakar S. Utility and limitations of glypican-3 expression for the diagnosis of hepatocellular carcinoma at both ends of the differentiation spectrum. Mod Pathol. 2008;21(8):1011–8.

    Article  CAS  PubMed  Google Scholar 

  18. Kim H, Park YN. Hepatocellular adenomas: recent updates. J Pathol Transl Med. 2021;55(3):171–80.

    Article  PubMed  PubMed Central  Google Scholar 

  19. Rodriguez EF, Fite JJ, Chowsilpa S, Maleki Z. Insulinoma-associated protein 1 immunostaining on cytology specimens: an institutional experience. Hum Pathol. 2019;85:128–35.

    Article  CAS  PubMed  Google Scholar 

  20. Leblebici C, Yeni B, Savli TC, Aydın Ö, Güneş P, Cinel L, Şimşek BÇ, Yıldız P, Tuncel D, Kayahan S. A new immunohistochemical marker, insulinoma-associated protein 1 (INSM1), for Merkel cell carcinoma: evaluation of 24 cases. Ann Diagn Pathol. 2019;40:53–8.

    Article  PubMed  Google Scholar 

  21. Gorbokon N, Timm P, Dum D, Menz A, Büscheck F, Völkel C, Hinsch A, Lennartz M, Luebke AM, Hube-Magg C, Fraune C, Krech T, Lebok P, Clauditz TS, Jacobsen F, Sauter G, Uhlig R, Steurer S, Minner S, Marx AH, Simon R, Burandt E, Bernreuther C, Höflmayer D. Mammaglobin-A expression is highly specific for tumors derived from the breast, the female genital tract, and the salivary gland. Diagnostics (Basel). 2023;13(6):1202.

    Article  CAS  PubMed  Google Scholar 

  22. Kinoshita Y, Hida T, Hamasaki M, Matsumoto S, Sato A, Tsujimura T, Kawahara K, Hiroshima K, Oda Y, Nabeshima K. A combination of MTAP and BAP1 immunohistochemistry in pleural effusion cytology for the diagnosis of mesothelioma. Cancer Cytopathol. 2018;126(1):54–63.

    Article  CAS  PubMed  Google Scholar 

  23. Weidemann S, Böhle JL, Contreras H, Luebke AM, Kluth M, Büscheck F, Hube-Magg C, Höflmayer D, Möller K, Fraune C, Bernreuther C, Rink M, Simon R, Menz A, Hinsch A, Lebok P, Clauditz T, Sauter G, Uhlig R, Wilczak W, Steurer S, Burandt E, Krech R, Dum D, Krech T, Marx A, Minner S. Napsin A expression in human tumors and normal tissues. Pathol Oncol Res. 2021;27:613099.

    Article  PubMed  PubMed Central  Google Scholar 

  24. Nakaguro M, Sadow PM, Hu R, Hattori H, Kuwabara K, Tsuzuki T, Urano M, Nagao T, Faquin WC. NKX3.1 expression in salivary gland “intraductal” papillary mucinous neoplasm: a low-grade subtype of salivary gland mucinous adenocarcinoma. Head Neck Pathol. 2022;16(4):1114–23.

    Article  PubMed  PubMed Central  Google Scholar 

  25. Haack H, Johnson LA, Fry CJ, Crosby K, Polakiewicz RD, Stelow EB, Hong SM, Schwartz BE, Cameron MJ, Rubin MA, Chang MC, Aster JC, French CA. Diagnosis of NUT midline carcinoma using a NUT-specific monoclonal antibody. Am J Surg Pathol. 2009;33(7):984–91.

    Article  PubMed  PubMed Central  Google Scholar 

  26. Hellquist H, French CA, Bishop JA, Coca-Pelaz A, Propst EJ, Paiva Correia A, Ngan BY, Grant R, Cipriani NA, Vokes D, Henrique R, Pardal F, Vizcaino JR, Rinaldo A, Ferlito A. NUT midline carcinoma of the larynx: an international series and review of the literature. Histopathology. 2017;70(6):861–8.

    Article  PubMed  PubMed Central  Google Scholar 

  27. Jones TD, Ulbright TM, Eble JN, Baldridge LA, Cheng L. OCT4 staining in testicular tumors: a sensitive and specific marker for seminoma and embryonal carcinoma. Am J Surg Pathol. 2004;28(7):935–40.

    Article  PubMed  Google Scholar 

  28. Pereira Pinto P, Zanine RM. Diagnostic value of p16 and Ki-67 expression in cervical glandular intraepithelial disease: a review. Ann Diagn Pathol. 2023;62:152054.

    Article  PubMed  Google Scholar 

  29. Cetani F, Banti C, Pardi E, Borsari S, Viacava P, Miccoli P, Torregrossa L, Basolo F, Pelizzo MR, Rugge M, Pennelli G, Gasparri G, Papotti M, Volante M, Vignali E, Saponaro F, Marcocci C. CDC73 mutational status and loss of parafibromin in the outcome of parathyroid cancer. Endocr Connect. 2013;2(4):186–95.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  30. Tazawa K, Kurihara Y, Kamoshida S, Tsukada K, Tsutsumi Y. Localization of prostate-specific antigen-like immunoreactivity in human salivary gland and salivary gland tumors. Pathol Int. 1999;49(6):500–5.

    Article  CAS  PubMed  Google Scholar 

  31. Miettinen M, Wang Z, McCue PA, Sarlomo-Rikala M, Rys J, Biernat W, Lasota J, Lee YS. SALL4 expression in germ cell and non-germ cell tumors: a systematic immunohistochemical study of 3215 cases. Am J Surg Pathol. 2014;38(3):410–20.

    Article  PubMed  PubMed Central  Google Scholar 

  32. Tacha D, Qi W, Zhou D, Bremer R, Cheng L. PAX8 mouse monoclonal antibody [BC12] recognizes a restricted epitope and is highly sensitive in renal cell and ovarian cancers but does not cross-react with b cells and tumors of pancreatic origin. Appl Immunohistochem Mol Morphol. 2013;21(1):59–63.

    Article  CAS  PubMed  Google Scholar 

  33. Magnusson K, de Wit M, Brennan DJ, Johnson LB, McGee SF, Lundberg E, Naicker K, Klinger R, Kampf C, Asplund A, Wester K, Gry M, Bjartell A, Gallagher WM, Rexhepaj E, Kilpinen S, Kallioniemi OP, Belt E, Goos J, Meijer G, Birgisson H, Glimelius B, Borrebaeck CA, Navani S, Uhlén M, O’Connor DP, Jirström K, Pontén F. SATB2 in combination with cytokeratin 20 identifies over 95% of all colorectal carcinomas. Am J Surg Pathol. 2011;35(7):937–48.

    Article  PubMed  Google Scholar 

  34. Miettinen M, McCue PA, Sarlomo-Rikala M, Biernat W, Czapiewski P, Kopczynski J, Thompson LD, Lasota J, Wang Z, Fetsch JF. Sox10—a marker for not only schwannian and melanocytic neoplasms but also myoepithelial cell tumors of soft tissue: a systematic analysis of 5134 tumors. Am J Surg Pathol. 2015;39(6):826–35.

    Article  PubMed  PubMed Central  Google Scholar 

  35. Crum NF. Coccidioidomycosis: a contemporary review. Infect Dis Ther. 2022;11(2):713–42.

    Article  PubMed  PubMed Central  Google Scholar 

  36. Bradsher RW. Histoplasmosis and blastomycosis. Clin Infect Dis. 1996;22(Suppl 2):S102–11.

    Article  PubMed  Google Scholar 

  37. Mittal J, Ponce MG, Gendlina I, Nosanchuk JD. Histoplasma capsulatum: mechanisms for pathogenesis. Curr Top Microbiol Immunol. 2019;422:157–91.

    CAS  PubMed  PubMed Central  Google Scholar 

  38. Ibrahim AS, Spellberg B, Walsh TJ, Kontoyiannis DP. Pathogenesis of mucormycosis. Clin Infect Dis. 2012;54(Suppl 1):S16–22.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  39. Kelly BT, Pennington KM, Limper AH. Advances in the diagnosis of fungal pneumonias. Expert Rev Respir Med. 2020;14(7):703–14.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  40. Smith S, Reuven N, Mohni KN, Schumacher AJ, Weller SK. Structure of the herpes simplex virus 1 genome: manipulation of nicks and gaps can abrogate infectivity and alter the cellular DNA damage response. J Virol. 2014;88(17):10146–56.

    Article  PubMed  PubMed Central  Google Scholar 

  41. de Melo Silva J, Pinheiro-Silva R, Dhyani A, Pontes GS. Cytomegalovirus and Epstein-Barr infections: prevalence and impact on patients with hematological diseases. Biomed Res Int. 2020;2020:1627824.

    Article  PubMed  PubMed Central  Google Scholar 

  42. Mehraein Y, Lennerz C, Ehlhardt S, Remberger K, Ojak A, Zang KD. Latent Epstein-Barr virus (EBV) infection and cytomegalovirus (CMV) infection in synovial tissue of autoimmune chronic arthritis determined by RNA- and DNA-in situ hybridization. Mod Pathol. 2004;17(7):781–9.

    Article  CAS  PubMed  Google Scholar 

  43. Clark NJ, Owada K, Ruberanziza E, Ortu G, Umulisa I, Bayisenge U, Mbonigaba JB, Mucaca JB, Lancaster W, Fenwick A, Soares Magalhães RJ, Mbituyumuremyi A. Parasite associations predict infection risk: incorporating co-infections in predictive models for neglected tropical diseases. Parasit Vectors. 2020;13(1):138.

    Article  PubMed  PubMed Central  Google Scholar 

  44. Hotez PJ, Bethony J, Bottazzi ME, Brooker S, Buss P. Hookworm: “the great infection of mankind”. PLoS Med. 2005;2(3):e67.

    Article  PubMed  PubMed Central  Google Scholar 

  45. Singhal AV, Sepulveda AR. Helicobacter heilmannii gastritis: a case study with review of literature. Am J Surg Pathol. 2005;29(11):1537–9.

    Article  PubMed  Google Scholar 

  46. Shah J, Shahidullah A. Ascaris lumbricoides: a startling discovery during screening colonoscopy. Case Rep Gastroenterol. 2018;12(2):224–9.

    Article  PubMed  PubMed Central  Google Scholar 

  47. National Nosocomial Infections Surveillance (NNIS) system report, data summary from January 1992–April 2000, issued June 2000. Am J Infect Control. 2000;28(6):429–48.

    Google Scholar 

  48. Wang HY, Kim H, Kim S, Bang H, Kim DK, Lee H. Evaluation of PCR-reverse blot hybridization assay for the differentiation and identification of Mycobacterium species in liquid cultures. J Appl Microbiol. 2015;118(1):142–51.

    Article  CAS  PubMed  Google Scholar 

  49. Martinez-Giron R, van Woerden HC, Pantanowitz L. Hematoidin crystals in sputum smears: cytopathology and clinical associations. Ann Thorac Med. 2020;15(3):155–62.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  50. Ali SY. Apatite-type crystal deposition in arthritic cartilage. Scan Electron Microsc. 1985;(Pt 4):1555–66.

    Google Scholar 

  51. Dieppe PA, Crocker PR, Corke CF, Doyle DV, Huskisson EC, Willoughby DA. Synovial fluid crystals. Q J Med. 1979;48(192):533–53.

    CAS  PubMed  Google Scholar 

  52. Chaplin AJ. Histopathological occurrence and characterisation of calcium oxalate: a review. J Clin Pathol. 1977;30(9):800–11.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  53. Schumacher HR Jr. Pathology of crystal deposition diseases. Rheum Dis Clin N Am. 1988;14(2):269–88. PMID: 3051152.

    Article  Google Scholar 

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Correspondence to Syed M. Gilani .

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Fatouh, K., Gilani, S.M. (2023). Quick Review. In: Gilani, S.M., Cai, G. (eds) Non-Neoplastic Cytology. Springer, Cham. https://doi.org/10.1007/978-3-031-44289-6_19

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  • DOI: https://doi.org/10.1007/978-3-031-44289-6_19

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