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Solid-State Nanopore Sensing Enhanced by Designed DNA Nanostructures

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Solid State Nanopores

Part of the book series: Nanostructure Science and Technology ((NST))

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Abstract

Solid-state nanopores are versatile single-molecule tools for label-free detection of biomolecules including DNA, RNA and protein by passing these molecules through nanopores using an applied electric potential. One of the main key challenges of solid-state nanopore in biosensing applications is their lack of specificity, as polymer chains with similar size and charge cause similar ionic current signals when they pass a nanopore. Thus, designed DNA nanostructures, named DNA carrier, which has specific binding sites that can selectively fish target molecules, were introduced to detect specific biomolecules such as protein and RNA. The DNA carrier attached with target protein generates a unique ionic current indicating the presence of the target and its location on the DNA carrier. Similarly, this system can be used to detect DNA sequence if functional protein molecules that can bind to specific DNA sequences are used to capture the DNA sequence. Moreover, the DNA carrier attached with DNA nanostructures has also find its way in the application as digital barcodes and for digital data storage. The use of DNA nanostructures significantly improves the specificity and versatility of solid-state nanopores in biosensing applications.

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Authors and Affiliations

  1. Cavendish Laboratory, University of Cambridge, Cambridge, UK

    Kaikai Chen & Ulrich F. Keyser

  2. School of Nanoscience and Nanotechnology, University of Chinese Academy of Sciences, Beijing, China

    Kaikai Chen

Authors
  1. Kaikai Chen
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  2. Ulrich F. Keyser
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Corresponding author

Correspondence to Ulrich F. Keyser .

Editor information

Editors and Affiliations

  1. Department of Electrical and Computer Engineering and Deptartment of Physics, University of Illinois Urbana-Champaign, Urbana, IL, USA

    Jean-Pierre Leburton

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Chen, K., Keyser, U.F. (2023). Solid-State Nanopore Sensing Enhanced by Designed DNA Nanostructures. In: Leburton, JP. (eds) Solid State Nanopores. Nanostructure Science and Technology. Springer, Cham. https://doi.org/10.1007/978-3-031-42336-9_4

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