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Defining the Assembleome of the Respiratory Syncytial Virus

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Virus Infected Cells

Part of the book series: Subcellular Biochemistry ((SCBI,volume 106))

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Abstract

During respiratory syncytial virus (RSV) particle assembly, the mature RSV particles form as filamentous projections on the surface of RSV-infected cells. The RSV assembly process occurs at the / on the cell surface that is modified by a virus infection, involving a combination of several different host cell factors and cellular processes. This induces changes in the lipid composition and properties of these lipid microdomains, and the virus-induced activation of associated Rho GTPase signaling networks drives the remodeling of the underlying filamentous actin (F-actin) cytoskeleton network. The modified sites that form on the surface of the infected cells form the nexus point for RSV assembly, and in this review chapter, they are referred to as the RSV assembleome. This is to distinguish these unique membrane microdomains that are formed during virus infection from the corresponding membrane microdomains that are present at the cell surface prior to infection. In this article, an overview of the current understanding of the processes that drive the formation of the assembleome during RSV particle assembly is given.

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Acknowledgments

We would also wish to acknowledge previous group members at the MRC Virology Unit in Glasgow and at the Nanyang Technological University in Singapore, including previous colleagues and collaborators Gaie Brown and Jim Aitkin, (Institute of Virology, Glasgow UK) and Chris Jeffree (EMCB, Biological Sciences EM Facility, University of Edinburgh UK) for light and electron microscopy imaging of RSV. In addition, we acknowledge previous funding support to RJS from the Medical Research Council (UK), National Medical Research Council (Singapore), Singapore-MIT Alliance for Research and Technology (Singapore), and the Ministry of Education (Singapore).

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Authors and Affiliations

  1. School of Biological Sciences, Nanyang Technological University, Singapore, Republic of Singapore

    Richard J. Sugrue

  2. LKC School of Medicine, Nanyang Technological University, Singapore, Republic of Singapore

    Boon Huan Tan

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  1. Richard J. Sugrue
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  2. Boon Huan Tan
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Correspondence to Richard J. Sugrue .

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Editors and Affiliations

  1. Centre for Virus Research (CVR), University of Glasgow, Glasgow, UK

    Swetha Vijayakrishnan

  2. Shanghai Institute of Immunity and Infection (Formerly Institut Pasteur of Shanghai), Chinese Academy of Sciences, Shanghai, China

    Yaming Jiu

  3. Institute for Molecular Physiology, University of Mainz, Mainz, Germany

    J. Robin Harris

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Sugrue, R.J., Tan, B.H. (2023). Defining the Assembleome of the Respiratory Syncytial Virus. In: Vijayakrishnan, S., Jiu, Y., Harris, J.R. (eds) Virus Infected Cells. Subcellular Biochemistry, vol 106. Springer, Cham. https://doi.org/10.1007/978-3-031-40086-5_9

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