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A Novel Algorithm for the Compensation of Hemoglobin Interference on Bilirubin Measurement Applied to a Two-Wavelengths Reflectance Photometer

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19th Nordic-Baltic Conference on Biomedical Engineering and Medical Physics (NBC 2023)

Part of the book series: IFMBE Proceedings ((IFMBE,volume 89))

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Abstract

Unconjugated Bilirubin (UCB) concentration in plasma can be higher in newborns than in adults; while a controlled and temporary increase of UCB concentration in plasma is physiological, a prolonged or excessive increase can lead to severe neurological damage or death. In high-resource settings several methods for bilirubin measurement have been in use for decades, including spectrophotometers that provide relatively accurate results and interference compensation of other analytes, such as hemoglobin. In Low- to Middle Income Countries (LMICs) however, the presence of expensive and complex laboratory instrumentation is not obvious. In this study a novel algorithm for the compensation of hemoglobin on bilirubin measurement was proposed and evaluated on a simple, inexpensive two-wavelengths reflectance photometer. Two bilirubin levels with five different hemoglobin concentrations were prepared; a linear function was used to approximate the relation between reflectance at 570 nm and hemoglobin concentration. The relative errors were reduced from 94% to 15.4% for the worst case. Further investigations are required to optimize the algorithm, including the use of different wavelengths and wider ranges of concentration.

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Authors and Affiliations

  1. Department of Engineering and Architecture, University of Trieste, Trieste, Italy

    Lorenzo Zucchini, Miloš Ajčević & Agostino Accardo

Authors
  1. Lorenzo Zucchini
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  2. Miloš Ajčević
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  3. Agostino Accardo
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Corresponding author

Correspondence to Lorenzo Zucchini .

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Editors and Affiliations

  1. Riga Technical University, Riga, Latvia

    Yuri Dekhtyar

  2. University of Latvia, Riga, Latvia

    Inga Saknite

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Zucchini, L., Ajčević, M., Accardo, A. (2023). A Novel Algorithm for the Compensation of Hemoglobin Interference on Bilirubin Measurement Applied to a Two-Wavelengths Reflectance Photometer. In: Dekhtyar, Y., Saknite, I. (eds) 19th Nordic-Baltic Conference on Biomedical Engineering and Medical Physics. NBC 2023. IFMBE Proceedings, vol 89. Springer, Cham. https://doi.org/10.1007/978-3-031-37132-5_3

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  • DOI: https://doi.org/10.1007/978-3-031-37132-5_3

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  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-031-37131-8

  • Online ISBN: 978-3-031-37132-5

  • eBook Packages: EngineeringEngineering (R0)

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