Abstract
Recently, nanofluidics exploiting 10–1000 nm spaces has developed and suggested a potential of novel analytical methods for pharmaceutical studies such as monitoring drug response of rare targets (extracellular vesicles, single cell, etc.) and ultrahigh-sensitivity assays of the diverse biological samples. A platform of nanofluidic device, where a network of micro- and nanochannels are fabricated on a substrate, allows analyses with minimal volumes (aL, fL, and pL) and a short analysis time (ms to min) by integrating chemical operations. In this chapter, fundamental methods and technologies of nanofluidics including device fabrication by top-down and bottom-up technologies, ultrasmall fluid manipulation by valves and liquid/liquid and gas/liquid interfaces, separation for biomolecules and nanoparticles, detection by electrical methods and optical methods and using analytical instruments, and high-throughput screening by an array of nanostructures in multi-nanofluidic channels are introduced. Applications of nanofluidics to single-cell analysis and shotgun proteomics, which are important for drug discovery and development to identify specific cellular responses to drug treatments, are presented.
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Kazoe, Y., Sueyoshi, K., Seetasang, S., Xu, Y. (2023). Nanofluidic Technologies for Drug Screening and Drug Delivery. In: Lamprou, D. (eds) Nano- and Microfabrication Techniques in Drug Delivery . Advanced Clinical Pharmacy - Research, Development and Practical Applications, vol 2. Springer, Cham. https://doi.org/10.1007/978-3-031-26908-0_14
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