Abstract
The L9(34) orthogonal experimental design was carried out with four factors including di-chloromethane-40% ammonia concentration ratio, solid-liquid ratio, extraction time and extraction power. The content of genistein, oxymatrine, matrine, daucostalin and chrysan-thin in the extract was calculated by information entropy weighting method, and the comprehensive score was obtained by weighted treatment. The optimum process was selected by orthogonal analysis with comprehensive score. The optimum extraction process was as follows: dichloromethane-40% ammonia concentration ratiois 25∶1.0, solid-liquid ratio is 1∶30, the extraction time is 20 min, and the extraction power is 80 W. The average value of five validation experiments was 0.180. The results showed that the optimized extraction process was stable and feasible, and the extraction rate was high.
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References
National Pharmacopoeia Committee Pharmacopoeia of the people’s Republic of China (Vol. I). China Pharmaceutical Science and Technology Press, Beijing, p. 27 (2015)
Gehongqiang. Clinical application and research of Radix Sophorae. Shizhen Nat. Med. (11), 1036 (2000)
Tan, C., Fang, H., Hutingjun.: Research progress on bioactive components and pharmacological effects of Radix Sophorae. J. Guangxi Agric. 40(11), 1494–1497 (2009)
Qian, L., Dai, W., Zhouguoqin, et al.: Study on analgesic and anti-inflammatory effects of main alkaloids of Sophora flavescens and Radix Sophorae. Chin. Patent Med. 34(8), 1593–1596 (2012)
Xiao, Z., Song, J., Xu, Z., et al.: Effects of water extract from Radix Sophorae on proliferation and metabolism of human hepatoma cells in vitro. J. Shandong Univ. Tradit. Chin. Med. 24(1), 63–65 (2000)
Xiao, P., Kubo, H., Ohsawa, M., et al.: Kappa-opioid receptor-mediated antinociceptive, effects of stereoisomers and derivatives of(+)-matrinein mice. Planta Med. 65(3), 230–233 (1999)
He, L.J., Liu, J.S., Luo, D., et al.: Quinolizidine alkaloids from Sophora tonkinensis and their anti - inflammatory activities. Fitoterapia 139, 104391 (2019)
Ding, P.L., Huang, H., Zhou, P., et al.: Quinolizidine alkaloids withanti - HBV activity from Sophora tonkinensis. Planta Med. 72(9), 854–856 (2006)
Dai, W., Qian, L., Yang, S., et al.: Study on antibacterial activity of alkaloids and total alkaloids from Sophora flavescens and Vicia faba root. Chin. J. Exp. Formulary 18(3), 177–180 (2012)
Peilan, D.: Comparative Study on Chemical Constituents of Radix Sophorae Flavescentis and Radix Sophorae Flavescentis. Fudan University, Shanghai (2004)
Li, X.N., Lu, Z.Q., Qin, S., et al.: Tonkinensines A and B, two novel alkaloids from Sophoratonkinensis. Tetrahed. Lett. 49(23), 3797–3801 (2008)
Li, X., Yan, H., Pang, X., et al.: Study on the chemical constituents of flavonoids in the root of Sophora. Chin. J. Tradit. Chin. Med. 34(3), 282–285 (2009)
Wang, J., Cui, Y.: Research progress on chemical constituents, pharmacological effects and toxicity of Radix Sophorae. Chin. J. Exp. Formulary 17(4), 229–232 (2011)
Yang, F.: Extraction and isolation of alkaloids from sophoraalopecuroides and their antioxidant and anti-tumor activities. Changchun Normal University, Changchun (2012)
Xu, H., Lu, J., Zhao, Y., et al.: Antioxidant and antibacterial activity of extracts from Sophoratonkinensis Gagnep. Food Ind. Sci. Technol. 36(14), 111–114 (2015)
Yanming, L., Wei, G.: Comparative study on different extraction methods of matrine from the root of kidney bean. Chem. Technol. Develop. 37(4), 22–24 (2008)
Huangjiwei, Qu., C., Zhuang, H., et al.: Optimization experiment on the decocting technology of Yam root. Tradit. Chin. Med. 28( 4), 339–340 (2005)
Yang, X., Bao, Y., Wang, S., et al.: Optimization of extraction process of total alkaloids from Radix Sophorae by central composite design response surface methodology. Shizhen National Med. 30(8), 1822–1824 (2019)
Meng, M., Wang, H., Ren, Y., et al.: Optimization of extraction technology of effective parts of Radix Sophorae by orthogonal test. Chin. J. Exp. Formulary 19(6), 46–49 (2013)
Wang, Y., Wang, Y., Zhu, B.: Microwave assisted extraction of matrine from the root of Sophora. Pesticides 50(5), 29–31 (2011)
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Wang, Y. et al. (2023). Optimization of Extraction Technology of Sophora Tonkinensis Gagnep. by Orthogonal Test Based on Information Entropy Weighting Method. In: Jan, M.A., Khan, F. (eds) Application of Big Data, Blockchain, and Internet of Things for Education Informatization. BigIoT-EDU 2022. Lecture Notes of the Institute for Computer Sciences, Social Informatics and Telecommunications Engineering, vol 467. Springer, Cham. https://doi.org/10.1007/978-3-031-23944-1_53
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