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CTC-Based Liquid Biopsies and Diagnostic Leukapheresis

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Circulating Tumor Cells

Part of the book series: Current Cancer Research ((CUCR))

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Abstract

Circulating tumor cells (CTCs) appear as ideal tissue surrogate to inform on molecular characteristics of systemic cancer for rational therapeutic decision-making. The full potential of CTCs for such liquid biopsies is currently limited by their low-frequency and unreliable detection even in metastatic cancer patients. A major reason for the low detection frequency is the low volume of a conventional blood sample not representative for the total blood volume in which the rare CTCs are unequally distributed. To resolve this problem, clinically safe and comfortable high blood volume analyses are required, and leukapheresis emerged as potential solution. The goal of this chapter is to inform the reader about the rationale, the underlying principle, first applications, and current challenges of diagnostic leukapheresis (DLA) to collect CTCs from large blood volumes, enhancing CTC detection and analysis for research and potential clinical applications.

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Acknowledgement

Figure 2 in this review was designed with the assistance of and modification to images provided by the Servier Medical Art database http://smart.servier.com/. Further information pertaining to the license and disclaimer notices can be found here: https://creativecommons.org/licenses/by/3.0/.

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  1. Experimental Surgical Oncology, Department for General, Visceral and Pediatric Surgery, University Hospital and Medical Faculty of the Heinrich-Heine-University Düsseldorf, Düsseldorf, Germany

    Nikolas H. Stoecklein

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  1. Nikolas H. Stoecklein
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Correspondence to Nikolas H. Stoecklein .

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  1. Department of Pathology and Immunology, Washington University in St. Louis School of Medicine, St. Louis, MO, USA

    Richard J. Cote

  2. ACTC Lab, Laboratory of Analytical Chemistry, Department of Chemistry, National and Kapodistrian University of Athens, Athens, Greece

    Evi Lianidou

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Stoecklein, N.H. (2023). CTC-Based Liquid Biopsies and Diagnostic Leukapheresis. In: Cote, R.J., Lianidou, E. (eds) Circulating Tumor Cells. Current Cancer Research. Springer, Cham. https://doi.org/10.1007/978-3-031-22903-9_1

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