Abstract
Saponins are group of chemical compounds found in many plants and some marine animals. Due to extensive biological activity, these compounds are widely used in pharmaceutical, food, cosmetic, and many other industries. Saponins are also known as biosurfactants. The word saponin implies a latin word “sapo” which means soap. Saponins are amphiphilic compounds containing hydrophilic and hydrophobic properties. One of the important characteristics of saponins is the foaming properties which makes them able to act as surface-active agents. Surface-active agents are widely used chemicals in different industrial processes. The purified Quillaja Saponin (QS-21) as a representative of plant saponins with high surface activity and biological properties has been proposed for industrial application as biosurfactant. This property of QS-21 is due to its high hydrophilic–lipophilic balance (HLB) value and lengthy sugar side chain. Formation of nanoemulsions using QS-21 saponin as biosurfactant has achieved more interest in recent studies. This chapter focused on saponin structure, surface activity, critical micelle concentration (CMC), rule of saponins as biosurfactants, and stabilization of nanoemulsions via saponin. The chapter also reviewed the Quillaja Saponin (QS-21) isolated from the bark of Quillaja saponaria Molina as a biosurfactant. It is expected that the future application of biosurfactants based on natural and less toxic bio-sources would become a topic of interest in food, cosmetic and pharmaceutical companies. However, further research should be carried out to highlight all the safety and side effects of saponin-based biosurfactants in future markets.
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References
Ahmadi O, Jafarizadeh-Malmiri H (2020) Green approach in food nanotechnology based on subcritical water: effects of thyme oil and saponin on characteristics of the prepared oil in water nanoemulsions. Food Sci Biotechnol 29(6):783–792
Akbari S, Abdurahman NH, Yunus RM (2019) Optimization of saponins, phenolics, and antioxidants extracted from fenugreek seeds using microwave-assisted extraction and response surface methodology as an optimizing tool. C R Chim 22(11–12):714–727
Akbari S, Abdurahman NH, Yunus RM, Fayaz F, Alara OR (2018) Biosurfactants—a new frontier for social and environmental safety: a mini review. Biotechnol Res Innov 2(1):81–90
Akbari S, Nour AH, Yunus RM, Farhan AH (2019b) Biosurfactants as promising multifunctional agent: a mini review. Int J Innov Res Sci Stud 1(1):1–6. https://doi.org/10.2139/ssrn.3323582
Aswathanarayan JB, Vittal RR (2019) Nanoemulsions and their potential applications in food industry. Front Sustain Food Syst 3:1–21
Augustin JM, Kuzina V, Andersen SB, Bak S (2011) Molecular activities, biosynthesis and evolution of triterpenoid saponins. Phytochemistry 72(6):435–457
Bernhoft A (2010) Bioactive compounds in plants - benefits and risks for man and animals. In The Norwegian Academy of Science and Letters, Oslo, Norway
Bhadoriya SS, Madoriya N (2013) Biosurfactants: a new pharmaceutical additive for solubility enhancement and pharmaceutical development. Biochem Pharmacol: Open Access 02:02
Boruah B, Gogoi M (2013) Plant based natural surfactants. Asian J Home Sci 8(2):759–762
Böttcher S, Drusch S (2017) Saponins—self-assembly and behavior at aqueous interfaces. Adv Colloid Interf Sci 243:105–113
Böttger S, Hofmann K, Melzig MF (2012) Saponins can perturb biologic membranes and reduce the surface tension of aqueous solutions : a correlation ? Bioorg Med Chem 20:2822–2828
Cheeke PR (2010) Actual and potential applications of Yucca schidigera and Quillaja saponaria saponins in human and animal nutrition. J Anim Sci 13:115–126
Chen XW, Wang JM, Guo J, Wan ZL, Yin SW, Yang XQ (2017) Hierarchical high internal phase emulsions and transparent oleogels stabilized by quillaja saponin-coated nanodroplets for color performance. Food Function 8(2):823–831
Chen Y, Miao Y, Huang L, Li J, Sun H, Zhao Y, Yang J, Zhou W (2014) Antioxidant activities of saponins extracted from radix Trichosanthis: an in vivo and in vitro evaluation. BMC Complement Altern Med 14:1–8
Cheok CY, Salman HAK, Sulaiman R (2014) Extraction and quantification of saponins: a review. Food Res Int 59:16–40
Dammak I, Sobral PJDA, Aquino A, das Neves MA, Conte-Junior CA (2020) Nanoemulsions: using emulsifiers from natural sources replacing synthetic ones—a review. Compr Rev Food Sci Food Saf 19(5):2721–2746
Den Brok MH, Büll C, Wassink M, De Graaf AM, Wagenaars JA, Minderman M, Thakur M, Amigorena S, Rijke EO, Schrier CC, Adema GJ (2016) Saponin-based adjuvants induce cross-presentation in dendritic cells by intracellular lipid body formation. Nat Commun 7(1):1–14
Dias B, Sales D (2017) Functional properties of saponins from sisal (Agave sisalana) and juá (ziziphus joazeiro): critical micellar concentration, antioxidant and antimicrobial activities colloids and surfaces a : physicochemical and engineering aspects functional properti. Colloids Surf A Physicochem Eng Asp 436:736–743
Doost AS (2018) Fabrication of Origanum compactum essential oil nanoemulsions stabilized using Quillaja saponin biosurfactant. J Food Process Preserv 42(7):e13668
Duan S, Jiang Y, Geng T, Ju H, Wang Y (2018) Wetting, foaming, and emulsification properties of novel methyltriphenylphosphonium carboxylate ionic liquid surfactants. J Dispers Sci Technol:1–7
Elazzazy AM, Abdelmoneim TS, Almaghrabi OA (2015) Isolation and characterization of biosurfactant production under extreme environmental conditions by alkali-halo-thermophilic bacteria from Saudi Arabia. Saudi J Biol Sci 22(4):466–475
Fracchia L, Banat J, Cavallo M, Ceresa C, Banat IM (2015) Potential therapeutic applications of microbial surface-active compounds. AIMS Bioeng 2(3):144–162
Fracchia L, Cavallo M, Giovanna M, & M., I. (2012). Biosurfactants and Bioemulsifiers Biomedical and Related Applications—Present Status and Future Potentials. In Biomedical Science, Engineering and Technology, 325–370
Goel PK (2012) Process for the extraction of furostanolic saponins from fenugreek seeds. U.S. Patent 8,217,165
Góral I, Wojciechowski K (2020) Surface activity and foaming properties of saponin-rich plants extracts. Adv Colloid Interf Sci 279:102145
Guclu-Ustundag Ö, Mazza G (2007) Saponins: properties, applications and processing. Crit Rev Food Sci Nutr 47(3):231–258
Gupta P, Khanday WA, Majid SA, Kushwa V, Tomar SS, Tomar R (2013) Study of sorption of metal oxoanions from waste water on surfactant modified analog of laumontite. J Environ Chem Eng 1(3):510–515
Jha SS, Joshi SJ, Geetha SJ (2016) Lipopeptide production by Bacillus subtilis R1 and its possible applications. Braz J Microbiol 47(4):955–964
Jiang Y, Geng T, Li Q, Li G, Ju H (2014) Influences of temperature , pH and salinity on the surface property and self-assembly of 1 : 1 salt-free catanionic surfactant. J Mol Liq 199:1–6
Kaur K, Kumar R, Mehta SK (2016) Formulation of saponin stabilized nanoemulsion by ultrasonic method and its role to protect the degradation of quercitin from UV light. Ultrason Sonochem 31:29–38
Kregiel D, Berlowska J, Witonska I, & Antolak H (2017) Saponin-based, biological-active surfactants from plants. In intechopen (pp. 183–205)
Kumar S, Yadav A, Yadav M, Yadav JP (2017) Effect of climate change on phytochemical diversity, total phenolic content and in vitro antioxidant activity of Aloe vera (L.) Burm.F. BMC Res Notes 10(1):1–12
Kumari S, Kumaraswamy RV, Choudhary RC, Sharma SS, Pal A, Raliya R, Biswas P, Saharan V (2018) Thymol nanoemulsion exhibits potential antibacterial activity against bacterial pustule disease and growth promotory effect on soybean. Sci Rep 8(1):1–12
Ledet G, Bostanian LA, & Mandal TK (2013) Nanoemulsions as a vaccine adjuvant. In CRC Press, 125–148
Liu Z, Li Z, Zhong H, Zeng G, Liang Y, Chen M, Wu Z, Zhou Y, Yu M, Shao B (2017) Recent advances in the environmental applications of biosurfactant saponins: a review. J Environ Chem Eng 5(6):6030–6038
Lorent JH, Quetin-Leclercq J, Mingeot-Leclercq MP (2014) The amphiphilic nature of saponins and their effects on artificial and biological membranes and potential consequences for red blood and cancer cells. Org Biomol Chem 12(44):8803–8822
Luo X, Zhou Y, Bai L, Liu F, Deng Y, Mcclements DJ (2016) Fabrication of β -carotene nanoemulsion-based delivery systems using dual-channel microfluidization : physical and chemical stability. J Colloid Interface Sci 490:328–335
Ma L, Liu H, Meng L, Qin P, Zhang B, Li Y, Man S, Liu Z, Liu Z, Diao A (2017) Evaluation of the anti-cancer activity of the triterpenoidal saponin fraction isolated from the traditional Chinese medicine Conyza blinii H. Lév. RSC Adv 7(6):3408–3412
Marchant R, Banat IM (2012) Biosurfactants: a sustainable replacement for chemical surfactants? Biotechnol Lett 34(9):1597–1605
Marrelli M, Conforti F, Araniti F, Statti GA (2016) Effects of saponins on lipid metabolism : a review of potential health benefits in the treatment of obesity. Molecules 21(10):1404
Mgbeahuruike EE, Yrjönen T, Vuorela H, Holm Y (2017) Bioactive compounds from medicinal plants: focus on piper species. S Afr J Bot 112:54–69
Mnif I, Ghribi D (2016) Glycolipid biosurfactants: main properties and potential applications in agriculture and food industry. J Sci Food Agric 96(13):4310–4320
Mohan VR, Tresina PS, Daffodil ED (2015) Antinutritional factors in legume seeds: characteristics and determination. Encyclopedia of Food and Health 1:211–220
Mondal MH, Malik S, Roy A, Saha R, Saha B (2015) Modernization of surfactant chemistry in the age of gemini and bio-surfactants: a review. RSC Adv 5(112):92707–92718
Moyo M, Amoo SO, Ncube B, Ndhlala AR, Finnie JF, Van Staden J (2013) Phytochemical and antioxidant properties of unconventional leafy vegetables consumed in southern Africa. S Afr J Bot 84:65–71
Mukherjee B, Chakraborty S, Mondal L, Satapathy BS, Sengupta S, Dutta L, Choudhury A, Mandal D (2016) Multifunctional drug nanocarriers facilitate more specific entry of therapeutic payload into tumors and control multiple drug resistance in cancer. Nanobiomaterials Cancer Ther:203–252
Mulligan CN (2005) Environmental applications for biosurfactants. Environ Pollut 133(2):183–198
Mulligan CN (2009) Recent advances in the environmental applications of biosurfactants. Curr Opin Colloid Interface Sci 14(5):372–378
Nagai T, Suzuki Y, Kiyohara H, Susa E, Kato T, Nagamine T, Hagiwara Y, Tamura SI, Yabe T, Aizawa C, Yamada H (2001) Onjisaponins, from the root of Polygala tenuifolia Willdenow, as effective adjuvants for nasal influenza and diphtheria-pertussis-tetanus vaccines. Vaccine 19(32):4824–4834
Navarro del Hierro J, Herrera T, Fornari T, Reglero G, Martin D (2018) The gastrointestinal behavior of saponins and its significance for their bioavailability and bioactivities. J Funct Foods 40:484–497
Nejatian M, Abbasi S (2019) Formation of concentrated triglyceride nanoemulsions and nanogels: natural emulsifiers and high power ultrasound. RSC Adv 9(49):28330–28344
Nitschke M, Costa SGVAO (2007) Biosurfactants in food industry. Trends Food Sci Technol 18(5):252–259
Oda K, Matsuda H, Murakami T, Katayama S, Ohgitani T, Yoshikawa M (2003) Relationship between adjuvant activity and amphipathic structure of soyasaponins. Vaccine 21(17–18):2145–2151
Oleszek WA (2002) Chromatographic determination of plant saponins. J Chromatogr A 967(1):147–162
Ozturk B, Argin S, Ozilgen M, Julian D (2015) Formation and stabilization of nanoemulsion-based vitamin E delivery systems using natural biopolymers : whey protein isolate and gum arabic. Food Chem 188:256–263
Pacheco GJ, Ciapina EMP, de Barros Gomes E, Pereira Junior N (2010) Biosurfactant production by Rhodococcus erythropolis and its application to oil removal. Braz J Microbiol 41(3):685–693
Pagureva N, Tcholakova S, Golemanov K, Denkov N, Pelan E, Stoyanov SD (2016) Surface properties of adsorption layers formed from triterpenoid and steroid saponins. Colloids Surf A Physicochem Eng Asp 491:18–28
Pathak K, Pattnaik S, & Swain K (2018) Application of nanoemulsions in drug delivery. In Nanoemulsions. Elsevier Inc.
Peter SP, Persson M, Claesson P, T. N (2004) Surface properties of surfactants derived from natural products. Part 1: syntheses and structure/property relationships—solubility and emulsification. J Surfactant Deterg 7(2):147–159
Piorkowski DT, McClements DJ (2013) Beverage emulsions: recent developments in formulation, production, and applications. Food Hydrocoll 42:5–41
Poojary MM, Vishnumurthy KA, Vasudeva Adhikari A (2015) Extraction, characterization and biological studies of phytochemicals from Mammea suriga. J Pharm Anal 5(3):182–189
Rekiel E, Smułek W, Zdziennicka A, Kaczorek E, Jańczuk B (2020) Wetting properties of Saponaria officinalis saponins. Colloids Surf A Physicochem Eng Asp 584(June 2019)
Riasat M, Heidari B, Pakniyat H, Jafari AA (2018) Assessment of variability in secondary metabolites and expected response to genotype selection in fenugreek (Trigonella spp.). Ind Crop Prod 123:221–231
Ross J, Miles GD (1941) An apparatus for comparison of foaming properties of soaps and detergents. Oil Soap 18(5):99–102
Salminen H, Bischoff S, Weiss J (2020) Formation and stability of emulsions stabilized by Quillaja saponin–egg lecithin mixtures. J Food Sci 85(4):1213–1222
Samal K, Das C, Mohanty K (2017) Eco-friendly biosurfactant saponin for the solubilization of cationic and anionic dyes in aqueous system dyes and pigments eco-friendly biosurfactant saponin for the solubilization of cationic and anionic dyes in aqueous system. Dyes Pigments 140(1):100–108
Schramm LL, Stasiuk EN, Marangoni DG (2003) 2 surfactants and their applications. Annu Rep Prog Chem, Sect C: Phys Chem 99:3–48
Schreiner TB, Santamaria-Echart A, Ribeiro A, Peres AM, Dias MM, Pinho SP, Barreiro MF (2020) Formulation and optimization of nanoemulsions using the natural surfactant saponin from Quillaja bark. Molecules 25(7):1–14
Scognamiglio M, Severino V, Abrosca BD, Chambery A, & Fiorentino A (2015) Structural elucidation of saponins : a combined approach based on high- resolution spectroscopic techniques. In Studies in Natural Products Chemistry (Vol. 45). Elsevier
Shah A, Shahzad S, Munir A, Nadagouda MN, Khan GS, Shams DF, Dionysiou DD, Rana UA (2016) Micelles as soil and water decontamination agents. Chem Rev 116(10):6042–6074
Shi J, Arunasalam K, Yeung D, Kakuda Y, Mittal G, Jiang Y (2004) Saponins from edible legumes: chemistry, processing, and health benefits. J Med Food 7(1):67–78
Sidana J, Singh B, Sharma OP (2016) Saponins of agave: chemistry and bioactivity. Phytochemistry 130:22–46
Singh B, Singh JP, Singh N, Kaur A (2017) Saponins in pulses and their health promoting activities: a review. Food Chem 233:540–549
Skountzou I, Brock N, Lelutiu N, & Compans RW (2017) Adjuvants for skin vaccination. In Immunopotentiators in Modern Vaccines: Second Edition, 399–419
Sonneville-aubrun O, Yukuyama MN, Pizzino A (2018) Application of nanoemulsions in cosmetics. Nano:435–475
Sun H, Yang Z, Ye Y (2006) Structure and biological activity of protopanaxatriol-type saponins from the roots of Panax notoginseng. Int Immunopharmacol 6(1):14–25
Sun HX, Xie Y, Ye YP (2009) Advances in saponin-based adjuvants. Vaccine 27(12):1787–1796
Uluata S, Mcclements DJ, Decker EA (2015) Physical stability , autoxidation and photosensitized oxidation of # -3 oils in nanoemulsion prepared with natural and synthetic surfactants physical stability , autoxidation and photosensitized oxidation of ω-3 oils in nanoemulsion prepared with natural. J Agric Food Chem 63(42):9333–9340
Varjani SJ, Upasani VN (2017) Critical review on biosurfactant analysis, purification and characterization using rhamnolipid as a model biosurfactant. Bioresour Technol 232:389–397
Ven HV, De D, Van L, Weyenberg W, Maes L, Ludwig A (2010) Nanosuspensions of chemically modified saponins: reduction of hemolytic side effects and potential tool in drug targeting strategy. J Control Release 148(1):e122–e123
Vincken JP, Heng L, de Groot A, Gruppen H (2007) Saponins, classification and occurrence in the plant kingdom. Phytochemistry 68(3):275–297
Williams JJ (2007) Formulation of carpet cleaners. In Handbook for Cleaning/Decontamination of Surfaces (Vol. 1)
Wojciechowski K, Orczyk M, Gutberlet T, Trapp M, Marcinkowski K, Kobiela T, Geue T (2014) Unusual penetration of phospholipid mono- and bilayers by Quillaja bark saponin biosurfactant. Biochim Biophys Acta Biomembr 1838(7):1931–1940
Yang D-z, Sun G, Zhang A, Shuang FJL (2013a) Screening and analyzing the potential bioactive components from rhubarb, using multivariate data processing approach and ultra-high performance liquid chromatography coupled with time-of-flight mass spectrometry. Anal Methods 68(1):100–100
Yang Y, Leser ME, Sher AA, McClements DJ (2013b) Formation and stability of emulsions using a natural small molecule surfactant: Quillaja saponin (Q-Naturale ®). Food Hydrocoll 30(2):589–596
Yang Y, Mcclements DJ (2013) Encapsulation of vitamin E in edible emulsions fabricated using a natural surfactant. Food Hydrocoll 30(2):712–720
Yu XL, He Y (2018) Tea saponins: effective natural surfactants beneficial for soil remediation, from preparation to application. RSC Adv 8(43):24312–24321
Zhang D, Zhang Q, Zheng Y, Lu J (2020) Anti-breast cancer and toxicity studies of total secondary saponin from anemone raddeana rhizome on MCF-7 cells via ROS generation and PI3K/AKT/mTOR inactivation. J Ethnopharmacol 259:112984
Zhang J, Bing L, Reineccius GA (2016) Comparison of modified starch and Quillaja saponins in the formation and stabilization of flavor nanoemulsions. Food Chem 192:53–59
Zhu Z, Wen Y, Yi J, Cao Y, Liu F (2018) Comparison of natural and synthetic surfactants at forming and stabilizing nanoemulsions : tea saponin , Quillaja saponin , and tween 80. J Colloid Interface Sci 536:80–87
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Sweeta Akbari is grateful from the Institute of International Education’s Scholar Rescue Fund (IIE-SRF) and Finnish National Agency for Education (EDUFI) for supporting her postdoctoral research in Tampere University.
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Akbari, S., Abdurahman, N.H., Kudrashou, V. (2023). Surface Activity and Emulsification Properties of Saponins as Biosurfactants. In: Aslam, R., Mobin, M., Aslam, J., Zehra, S. (eds) Advancements in Biosurfactants Research. Springer, Cham. https://doi.org/10.1007/978-3-031-21682-4_7
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