Abstract
Sorghum is a very heat-loving crop, therefore, at low positive temperatures, its seeds germinate for a long time, the plants grow and develop slowly, which leads to the overgrowth of the field with weeds. Despite the thermophilicity of sorghum, its plants are capable of hardening. Sudan grass is more cold hardy than grain and sugar sorghum. Thus, the search for and identification of cold-resistance genes in Sudan grass samples is an urgent task for the further development of cold-resistant varieties. The aim of the research is to identify the cold tolerance of Sudan grass samples using physiological methods and Fearlygerm-9.3 QTL markers. When studying the samples, the following laboratory methods were used: seed germination at low temperatures, DNA isolation, PCR, and electrophoresis on agarose gels. The cold resistance gene was determined using the Xsbarslbk 9.58 and Xsbarslbk 2.56 markers. All studied genotypes were divided into 5 groups according to cold resistance. As a result of the comprehensive studies, cold-resistant samples of Sudanese grass were identified: Volzhskaya 51, Senokosny, K-315, Gratsiya, Priobskaya, Yubileinaya 20, K-160, K-279. The isolated samples of Sudanese grass can be used in breeding programs aimed at creating cold-resistant varieties and hybrids.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Burke, J.J., Emendack, Y.Y., Hayes, C.M., Xin, Z., Burow, G.B.: Registration of five grain sorghum lines with early-season cold tolerance. J. Plant Reg. 13(3), 422–426 (2019)
Maulana, F., Tesso, T.: Cold temperature episode at seedling and fowering stages reduces growth and yield components in sorghum Crop. Sci. 53, 564–574 (2013)
Awad, A., Hafiz, S., Hammada, M.A.S., El-Nouby, E.-H., A,: Grain yield production of Sudan grass (Sorghum sudanense (Piper) Stapf) as influenced by cutting numbers, potassium rates, and intrarow spacing in a semiarid environment. Turk. J. Agric. For. 37(6), 657–664 (2013). https://doi.org/10.3906/tar-1208-53
Emendack, Y.Y., Burke, J.J., Sanchez, J., Laza, H.E., Hayes, C.M.: Agro-morphological characterization of diverse sorghum lines for pre-and post-fowering drought tolerance Aust. J. Crop Sci. 12(1), 135–150 (2018)
Emendack, Y., Sanchez, J., Hayes, C., Nesbitt, M., Laza, H., Burke, J.: Seed-to-seed early-season cold resiliency in sorghum. Sci. Rep. 11, 7801 (2021). https://doi.org/10.1038/s41598-021-87450-1
Emendack, Y.Y., Malinowski, D.P., Burke, J.J., Burow, G.B., Xin, Z.: Morpho-physiological characterization of cold and prefowering drought stress tolerance in grain sorghum (Sorghum bicolor (L.) Moench) inbreds. Am. J. Exp. Agric. 4(12), 1500–1516 (2014)
Chiluwal, A., et al.: Integrated aerial and destructive phenotyping differentiates chilling stress tolerance during early seeling growth in sorghum. Field Crops Res 227, 1–10 (2018)
Kapanigowda MH et al. (2013) Analyses of sorghum (Sorghum bicolor (L.) Moench) lines and hybrid in response to early season planting and cool conditions, Can. J. Plant Sci. 93:773
Ortiz, D., Hu, J., Fernandez, M.G.S.: Genetic architecture of photosynthesis in Sorghum bicolor under non-stress and cold conditions. J. Exp. Bot. 68, 4545–4557 (2017)
Phuong, N., Stuzel, H., Uptmoor, R.: Quantitative trait loci associated to agronomic traits and yield components in a Sorghum bicolor L Moench RIL population cultivated under pre-flowering drought and well-watered conditions. J. Agric. Sci. 4, 781–791 (2013). https://doi.org/10.4236/as.2013.412107
Gutema, Z., Assefa, T., Fu, F.: Detection of quantitative trait loci (QTL) associated with yield and yield component traits in sorghum [Sorghum bicolor (L.) Moench] sown early and late planting dates. J. Exper. Biol. Agric.Sci. 4(1), 26–36 (2016)
Fernandez, M.G.S., Schoenbaum, G.R., Goggi, A.S.: Novel germplasm and screening methods for early cold tolerance in sorghum. Crop Sci. 54, 2631–2638 (2014)
Shehzad, T., Okuno, K.: QTL mapping for yield and yield-contributing traits in sorghum (Sorghum bicolor (L.) Moench) with genome-based SSR markers. Euphytica 203(1), 17–31 (2014). https://doi.org/10.1007/s10681-014-1243-9
Zou, G., et al.: Identification of QTLs for eight agronomically important traits using an ultrahighdensity map based on SNPs generated from high-throughput sequencing in sorghum under contrasting photoperiods. J. Exp. Bot. 63(15), 5451–5462 (2012). https://doi.org/10.1093/jxb/ers205
Yılmaz, M., Ozic, C., Gok, I.: Principles of Nucleic acid separation by agarose gel electrophoresis. In: book: Gel Electrophoresis - Principles and Basics Edition: Sameh Magdeldin, Publisher: INTECH Chapter 3, 33–40 (2012). https://doi.org/10.5772/38654
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2023 The Author(s), under exclusive license to Springer Nature Switzerland AG
About this paper
Cite this paper
Kostylev, P., Kupreyshvili, N., Kovtunova, N., Zhogaleva, O. (2023). Physiological and Genetic Evaluation of Sudan Grass Samples for Cold Hardiness. In: Beskopylny, A., Shamtsyan, M., Artiukh, V. (eds) XV International Scientific Conference “INTERAGROMASH 2022”. INTERAGROMASH 2022. Lecture Notes in Networks and Systems, vol 574. Springer, Cham. https://doi.org/10.1007/978-3-031-21432-5_319
Download citation
DOI: https://doi.org/10.1007/978-3-031-21432-5_319
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-031-21431-8
Online ISBN: 978-3-031-21432-5
eBook Packages: EngineeringEngineering (R0)