Abstract
Colorectal carcinoma cell lines differ in invasiveness and migratory potential. Cell motility characterizes the tissue and specific resistance to genotoxic agents and protects cancer cells from the micro-environmental impact, especially from the extracellular matrix. The regulatory interactions, described as signalization loops comprised of released adipocytokines and specific for them receptors. The responses could be regulated by positive feedback, e.g., when visfatin/vaspin stimulates (up-regulates) the expression of a gene (during the incubation of cells with visfatin or vaspin), or by negative feedback when visfatin/vaspin inhibits (down-regulates) the expression of a gene. The strongest stimulation of metalloproteinases was observed under vaspin treatment in HCT116 (Duke’s stage A), in LS-180 (Duke’s stage B), and the weakest in HCA-2 (Duke’s stage C), in comparison to the untreated control cells. Metalloproteinase gene expression in cell lines was connected to cancer invasiveness and Duke’s grading system for colorectal cancers.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Dukes, C.E.: The classification of cancer of the rectum. J. Pathol. 35, 323–332 (1932)
Matsuyama, Y., Takao, S., Aikou, T.: Comparison of matrix metalloproteinase expression between primary tumors with or without liver metastasis in pancreatic and colorectal carcinomas. J. Surg. Oncol. 80(2), 105–110 (2002)
Li, B.H., Zhao, P., Liu, S.Z., Yu, Y.M., Han, M., Wen, J.K.: Matrix metalloproteinase-2 and tissue inhibitor of metalloproteinase-2 in colorectal carcinoma invasion and metastasis. World J. Gastroenterol. 11, 3046–50 (2005)
Korbicz, J., Maniewski, R., Patan, K., Kowal, M. (eds.); Current trends in biomedical engineering and bioimages analysis. In: Proceedings of the 21st Polish Conference on Biocybernetics and Biomedical Engineering (PCBEE 2019). AISC, vol. 1033. Springer, Cham (2020). https://doi.org/10.1007/978-3-030-29885-2
Skonieczna, M., Hudy, D., Hejmo, T., Buldak, R.J., Adamiec, M., Kukla, M.: The adipokine vaspin reduces apoptosis in human hepatocellular carcinoma (Hep-3B) cells, associated with lower levels of NO and superoxide anion. BMC Pharmacol. Toxicol. 20, 58 (2019)
Skonieczna, M., Adamiec, M., Hudy, D., Niesłoń, P., Fochtman, D., Bil, P.: Live impedance measurements and time-lapse microscopy observations of cellular adhesion, proliferation and migration after ionizing radiation. Curr. Pharm. Biotechnol. 21(7), 642–652 (2020)
Ehrig, K., Kilinc, M.O., Chen, N.G., et al.: Growth inhibition of different human colorectal cancer xenografts after a single intravenous injection of oncolytic vaccinia virus GLV-1h68. J Transl Med 11, 79 (2013)
Yusof, H.M., Ab-Rahim, S., Wan Ngah, W.Z., Nathan, S., Jamal, A.R.A., Mazlan, M.: Metabolites profile of colorectal cancer cells at different stages. Int. J. Appl. Pharm. 11(5), 66–70 (2019)
Shabahang, M., Buras, R.R., Davoodi, F., Schumaker, L.M., Nauta, R.J., Evans, S.R.T.: 1,25-Dihydroxyvitamin D3 receptor as a marker of human colon carcinoma cell line differentiation and growth inhibition, Cancer Res. 53, 3712–3718 (1993)
Ferenac, M., Polančec, D., Huzak, M., Pereira-Smith, O.M., Rubelj, I.: Early-senescing human skin fibroblasts do not demonstrate accelerated telomere shortening. J. Gerontol. Ser. A 60(7), 820–829 (2005)
Gupta, A., Mugundu, G.M., Desai, P.B., Thummel, K.E., Unadkat, J.D.: Intestinal human colon adenocarcinoma cell line LS180 is an excellent model to study pregnane X receptor, but not constitutive androstane receptor, mediated CYP3A4 and multidrug resistance transporter 1 induction: studies with anti-human immunodeficiency virus protease inhibitors. Drug Metab. Dispos. 36(6), 1172–80 (2008)
Bułdak, R.J., et al.: Changes in subcellular localization of visfatin in human colorectal HCT-116 carcinoma cell line after cytochalasin B treatment. Eur. J. Histochem. 58(3), 2408 (2014)
Buldak, R.J., et al.: Exogenous administration of visfatin affects cytokine secretion and increases oxidative stress in human malignant melanoma Me45 cells. J. Physiol. Pharmacol. 64(3), 377–385 (2013)
Douglas, H., Robert, A.: Weinberg Cell, vol. 100, pp. 57–70, Cell Press (2000)
Douglas, H., Robert, A.: Weinberg Cell, vol. 144, Elsevier Inc., New York (2011)
Acknowledgement
Students’ Scientific Society of Biotechnology was granted support in the I edition of funding for student research and in the II edition of funding for Project-Based Learning under the Excellence Initiative - Research University program, by Rector’s of the Silesian University of Technology Regulation No. 54/2020 and 55/2020 issued March 13, 2020, and grants \(02/040/BK\_20/0002\) (Magdalena Skonieczna and Dorota Hudy), and \(02/040/BKM\_20/0005\) (Małgorzata Adamiec).
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2022 The Author(s), under exclusive license to Springer Nature Switzerland AG
About this paper
Cite this paper
Gałecki, S. et al. (2022). Adipocytokine Vaspin Stimulates the Migration Process in Different Colorectal Cancer Cell Lines. In: Piaseczna, N., Gorczowska, M., Łach, A. (eds) Innovations and Developments of Technologies in Medicine, Biology and Healthcare. EMBS ICS 2020. Advances in Intelligent Systems and Computing, vol 1360. Springer, Cham. https://doi.org/10.1007/978-3-030-88976-0_4
Download citation
DOI: https://doi.org/10.1007/978-3-030-88976-0_4
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-030-88975-3
Online ISBN: 978-3-030-88976-0
eBook Packages: Intelligent Technologies and RoboticsIntelligent Technologies and Robotics (R0)