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Fluorescent Probes for Monitoring Oxidation of Lipids and Assessment of Antioxidant Activity

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Lipid Oxidation in Food and Biological Systems

Abstract

Knowledge of the kinetics and mechanism of peroxidation and antioxidant action gained from model systems (solvents, micelles, liposomes) is fundamental for the exploration of processes mediated by Reactive Oxygen Species (ROS) in the cells and organisms. The research concerning ROS-related biological functions and harmful effects continuously requires new sensitive and specific tools for fast and reliable monitoring of inhibited and non-inhibited oxidation and tools to enable a deeper insight into the molecular basis of ROS-related diseases. Fluorescent probes (FP) are promising sensors of ROS due to their high sensitivity, simplicity in data collection, and high spatial resolution in imaging techniques. This chapter provides a brief description of the development of FPs applied for sensing selected radical and non-radical ROS (superoxide O2•−, hydroxyl radical HO•, lipid peroxyl radicals LOO•, hydrogen peroxide H2O2, and alkyl hydroperoxides ROOH). The achievements in the design of FPs have elevated the methodology of research from simple fluorimetric titrations to the methods approaching the sensitivity on the level of single molecules observed in individual liposomes or living cells and also to new techniques for monitoring the rate of oxidation and determination of the kinetic parameters with precision comparable to conventional, non-fluorescent methods.

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Acknowledgements

Financial support from the National Science Centre, Poland (NCN grant No. 2018/31/B/ST4/02354) is gratefully acknowledged.

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Authors and Affiliations

  1. University of Warsaw, Faculty of Chemistry, Warsaw, Poland

    Jarosław Kusio & Grzegorz Litwinienko

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  1. Jarosław Kusio
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  2. Grzegorz Litwinienko
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Correspondence to Grzegorz Litwinienko .

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  1. Facultad de Química, Dpto Química Física, Universidad de Vigo, Vigo, Spain

    Carlos Bravo-Diaz

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Kusio, J., Litwinienko, G. (2022). Fluorescent Probes for Monitoring Oxidation of Lipids and Assessment of Antioxidant Activity. In: Bravo-Diaz, C. (eds) Lipid Oxidation in Food and Biological Systems. Springer, Cham. https://doi.org/10.1007/978-3-030-87222-9_3

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