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Laboratory Protocols in Fungal Biology pp 209–234Cite as

Fluorochrome-Based Methods for Fungal Sample Examination

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Part of the book series: Fungal Biology ((FUNGBIO))

Abstract

This chapter presents methods for fungi and plants infected with fungi analysis using epi-fluorescence (EFM) and laser scanning confocal microscopy (LSM). Here are included fluorochrome-based methods for evidencing fungi structures as well as cell wall and nuclei, besides reactive oxygen species and cellular death staining. Plant dyes examples important for fungi-plant interaction studies and autofluorescent structures for certain fungi are shown.

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Acknowledgements

The authors gratefully acknowledge CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico), CAPES (Coordenadoria de Aperfeiçoamento de Pessoal de Nível Superior), FAPEMIG (Fundação de Amparo à Pesquisa do Estado de Minas Gerais—Brazil) and FINEP (Financiadora de Estudos e Projetos) for financial support. We thanks also Aline Ferreira and Elisa Castro, technicians at the Electron Microscopy and Ultrastructural Analysis lab at UFLA, Brazil.

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Authors and Affiliations

  1. Department of Plant Pathology, Electron Microscopy, and Ultrastructural Analysis Lab, Federal University of Lavras (UFLA), Lavras, Minas Gerais, Brazil

    Silvino Intra Moreira & Eduardo Alves

  2. Department of Biology, Plant Disease Resistance Lab, Federal University of Lavras (UFLA), Lavras, Minas Gerais, Brazil

    Lucas Fidelis Pereira & Elaine Aparecida de Souza

Authors
  1. Silvino Intra Moreira
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  2. Lucas Fidelis Pereira
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  3. Elaine Aparecida de Souza
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  4. Eduardo Alves
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Corresponding author

Correspondence to Eduardo Alves .

Editor information

Editors and Affiliations

  1. Center for Safe and Improved Food & Biorefining and Advanced Materials Research Center, SRUC, Edinburgh, Scotland, UK

    Vijai Kumar Gupta

  2. Department of Biochemistry, National University of Ireland Galway, Galway, Ireland

    Maria Tuohy

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Moreira, S.I., Pereira, L.F., de Souza, E.A., Alves, E. (2022). Fluorochrome-Based Methods for Fungal Sample Examination. In: Gupta, V.K., Tuohy, M. (eds) Laboratory Protocols in Fungal Biology. Fungal Biology. Springer, Cham. https://doi.org/10.1007/978-3-030-83749-5_11

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