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Laboratory Protocols in Fungal Biology pp 1–67Cite as

Various Methods of Long-Term Preservation of Fungal Cultures in All-Russian Collection of Microorganisms (VKM)

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Part of the book series: Fungal Biology ((FUNGBIO))

Abstract

The results of successful long-term preservation by various methods (cryopreservation, lyophilization, preservation on anhydrous silica gel and in sterile soil) are presents for different taxonomic groups of fungi preserved in All-Russian Collection of Microorganisms (VKM), G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences (IBPM RAS). For each species, data are given on the number of strains studied and the maximum time of maintaining vitality. The protocol of method “drying on anhydrous silica gel” with the following storage at different temperatures is given.

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Author information

Authors and Affiliations

  1. All-Russian Collection of Microorganisms (VKM), G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Science, Pushchino, Moscow Region, Russia

    Svetlana M. Ozerskaya, Nataliya E. Ivanushkina, Galina A. Kochkina, Anastasya A. Danilogorskaya, Irina P. Pinchuk & Alexander N. Vasilenko

Authors
  1. Svetlana M. Ozerskaya
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  2. Nataliya E. Ivanushkina
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  3. Galina A. Kochkina
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  4. Anastasya A. Danilogorskaya
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  5. Irina P. Pinchuk
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  6. Alexander N. Vasilenko
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Corresponding author

Correspondence to Svetlana M. Ozerskaya .

Editor information

Editors and Affiliations

  1. Center for Safe and Improved Food & Biorefining and Advanced Materials Research Center, SRUC, Edinburgh, Scotland, UK

    Vijai Kumar Gupta

  2. Department of Biochemistry, National University of Ireland Galway, Galway, Ireland

    Maria Tuohy

Annexies

Annexies

1.1.1 Annex 1: Fields Attributes in the Table «Database Preservation Methods»

Code

Counter

EntryDate

Date/Time

Method

Text

Col

Text

Strain

Numerical

dep

Text

pat

Text

Curator

Text

Dubl-cart

Text

Dubl fond

Text

Dubl fond new

Text

Ampules

Numerical

Data

Date/Time

Result

Text

Data2

Date/Time

Result2

Text

Days

Numerical

Year

Numerical

Comments

Text

Data3

Date/Time

Result3

Text

Data4

Date/Time

Result4

Text

Data5

Date/Time

Result5

Text

EditDate

Date/Time

Protector

Text

Programm

Text

Location

Text

Type

Text

1.1.2 Annex 2: Maximal Preservation Times for VKM Fungal Species

Table 4
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Ozerskaya, S.M., Ivanushkina, N.E., Kochkina, G.A., Danilogorskaya, A.A., Pinchuk, I.P., Vasilenko, A.N. (2022). Various Methods of Long-Term Preservation of Fungal Cultures in All-Russian Collection of Microorganisms (VKM). In: Gupta, V.K., Tuohy, M. (eds) Laboratory Protocols in Fungal Biology. Fungal Biology. Springer, Cham. https://doi.org/10.1007/978-3-030-83749-5_1

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