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Tissue Microarray

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Handbook of Practical Immunohistochemistry

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Abstract

Tissue microarray (TMA) is a powerful tool for performing population level studies using tissues routinely processed in surgical pathology and cytopathology cell blocks. TMA construction can be done manually or with semiautomated and fully automated equipment on formalin fixed, paraffin embedded (FFPE) tissue produced in routine histology. A variety of other processed materials may also be used, including frozen tissue, cell culture lines, and resin embedded tissue. TMAs are used for a variety of applications including the validation of cDNA array data; validation of the sensitivity and specificity of antibodies; quality assurance in immunohistochemistry; translation of data from cell line, xenograft, and animal models to human cancer; collaborative studies, especially for the aggregation and preservation of rare tumor tissues; molecular profiling of large series of tumors or diseased tissue and correlation with clinical endpoints; and evaluation of diagnostic, prognostic, and therapeutic potential of newly discovered genes and molecules. Most standard histologic and molecular techniques can be applied to TMA sections. Optimal storage conditions of TMA blocks and sections in order to preserve antigenicity continue to be an issue. Image analysis and data management are crucial issues, but many tools are available.

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Authors and Affiliations

  1. Department of Laboratory Medicine, Geisinger Medical Center, Danville, PA, USA

    Myra L. Wilkerson

  2. Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, Bethesda, MD, USA

    Stephen Hewitt

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  1. Myra L. Wilkerson
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Correspondence to Myra L. Wilkerson .

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Editors and Affiliations

  1. Department of Laboratory Medicine, Geisinger Medical Center, Danville, PA, USA

    Fan Lin

  2. Department of Laboratory Medicine, Geisinger Medical Center, Danville, PA, USA

    Jeffrey W. Prichard

  3. Department of Laboratory Medicine, Geisinger Medical Center, Danville, PA, USA

    Haiyan Liu

  4. Department of Laboratory Medicine, Geisinger Medical Center, Danville, PA, USA

    Myra L. Wilkerson

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Wilkerson, M.L., Hewitt, S. (2022). Tissue Microarray. In: Lin, F., Prichard, J.W., Liu, H., Wilkerson, M.L. (eds) Handbook of Practical Immunohistochemistry. Springer, Cham. https://doi.org/10.1007/978-3-030-83328-2_11

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