Skip to main content
SpringerLink
Log in

Bioactive Anticancer Peptides in Soybean Seeds

  • Chapter
  • First Online:
Soybean Seed Composition

  • 385 Accesses

Abstract

In previous chapters, we summarized studies showing the beneficial effects of isofalvones on human health and on the prevention of human diseases, including cancer, cardiovascular diseases, osteoporosis, menopausal symptoms and menstrual cycles in women, thyroid problems, memory and learning disability, problems in brain development, Alzheimer’s disease, obesity, reproductive problems, bone density issues, diabetes, immunity deficiency, and so on. In addition, the therapeutic and anticancer functions of soybean have also been attributed to bioactive peptides such as lunasin and BBI (Bowman-Birk protease inhibitor) found both in seed protein and gastrointestinal digestive tract. In this chapter, we summarize the anticancer properties of soybean seed peptides such as BBI and lunasin, current understanding of their genetic inheritance, and molecular analysis and quantification of lunasin in soybean seeds. Currently, we are investigating the QTN (quantitative trait nucleotide) mapping of seed lunasin content in our department using multi-locus genome wide association (GWAS) approach. These studies will help in developing soybean lines with high seed lunasin content in the future.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 149.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 199.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 199.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  • Anders, S., and W. Huber. 2010. Differential expression analysis for sequence count data. Genome Biology 11: R106.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Birk, Y., A. Gertler, and S. Khalef. 1963. A pure trypsin inhibitor from soya bean. The Biochemical Journal 87: 281–284.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Bowman, D.E. 1946. Differentiation of soybean antitryptic factor. Proceedings of the Society for Experimental Biology and Medicine 63: 547–550.

    Article  CAS  PubMed  Google Scholar 

  • Chatterjee, C., S. Gleddie, and C.W. Xiao. 2018. Soybean bioactive peptides and their functional properties. Nutrients 10 (9): 1211. https://doi.org/10.3390/nu10091211.

    Article  CAS  PubMed Central  Google Scholar 

  • Cruz-Huerta, E., S. Fernández-Tomé, M.C. Arques, L. Amigo, I. Recio, A. Clemente, and B. Hernández-Ledesma. 2015. The protective role of the Bowman-Birkprotease inhibitor in soybean lunasin digestion: The effect of released peptides on colon cancer growth. Food & Function 6: 2626–2635. https://doi.org/10.1039/c5fo00454c.

    Article  CAS  Google Scholar 

  • Csanádi, G.J., J. Vollmann, G. Stift, and T. Lelley. 2001. Seed quality QTLs identified in a molecular map of early maturing soybean. Theoretical and Applied Genetics 103: 912–919.

    Article  Google Scholar 

  • de Lumen, B.O. 2005. Lunasin: A cancer-preventive soy peptide. Nutrition Reviews 63: 16–21.

    Article  PubMed  Google Scholar 

  • de Mejia, E.G., T. Bradford, and C. Hasler. 2003. The Anticarcinogenic Potential of Soybean Lectin and Lunasin. Nutrition Reviews 61 (7): 239–246. https://doi.org/10.1301/nr.2003.jul.239-246.

  • de Mejia, G.E., M. Vásconez, B.O. de Lumen, and R. Nelson. 2004. Lunasin concentration in different soybean genotypes, commercial soy protein, and isoflavone products. Journal of Agricultural and Food Chemistry 52: 5882–5887.

    Article  CAS  Google Scholar 

  • Devapatla, B., C. Shidal, K. Yaddanapudi, and K. Davis. 2019. F1000Research. https://doi.org/10.12688/f1000research.9661.2.

  • Dia, V.P., D. Torres, B.O. de Lumen, J.W. Erdman, and E.G. de Mejia. 2009. Presence of lunasin in plasma of men after soy protein consumption. Journal of Agricultural and Food Chemistry 57: 1260–1266.

    Article  CAS  PubMed  Google Scholar 

  • Diers, B.W., P. Keim, W.R. Fehr, and R.C. Shoemaker. 1992. RFLP analysis of soybean seed protein and oil content. Theoretical and Applied Genetics 83: 608–612.

    Article  CAS  PubMed  Google Scholar 

  • Ďúranová, H., V. Fialková, J. Bilčíková, N. Lukáč, and Z Kňažická. 2019 Lunasin and its versatile health-promoting actions. Journal of Microbiology, Biotechnology and Food Sciences. https://doi.org/10.15414/jmbfs.2019.8.4.1106-1110.

  • Fernández-Tomé, S., and B. Hernández-Ledesma. 2019. Current state of art after twenty years of the discovery of bioactive peptide lunasin. Food Research International 116: 71–78.

    Article  PubMed  CAS  Google Scholar 

  • Galvez, A.F., and B.O. de Lumen. 1999. A soybean cDNA encoding a chromatin-binding peptide inhibits mitosis of mammalian cells. Nature Biotechnology 17: 495–500.

    Article  CAS  PubMed  Google Scholar 

  • Galvez, A.F., M. Revilleza, and B.O. de Lumen. 1997. A novel methionine-rich protein from soybean cotyledon: Cloning and characterization of cDNA (accession No. AF005030). Plant gene register #PGR97-103. Plant Physiology 114: 1567–1569.

    Article  Google Scholar 

  • Galvez, A.F., N. Chen, J. Macasieb, and B.O. de Lumen. 2001. Chemopreventive property of a soybean peptide (lunasin) that binds to deacetylated histones and inhibits acetylation. Cancer Research 61: 7473–7478.

    CAS  PubMed  Google Scholar 

  • Gizlice, Z., T.E. Carter, and J.W. Burton. 1994. Genetic base for North American public soybean cultivars released between 1947 and 1988. Crop Science 34: 1143–1151.

    Article  Google Scholar 

  • Greenwald, P., C.K. Clifford, and J.A. Milner. 2001. Diet and cancer prevention. European Journal of Cancer 37 (8): 948–965.

    Article  CAS  PubMed  Google Scholar 

  • Guo, Y., B. Su, J. Tang, F. Zhou, and L.J. Qiu. 2018. Gene-based SNP identification and validation in soybean using next-generation transcriptome sequencing. Molecular Genetics and Genomics 293 (3): 623–633.

    Article  CAS  PubMed  Google Scholar 

  • He, F.J., and J.Q. Chen. 2013. Consumption of soybean, soy foods, soy isoflavones and breast cancer incidence: Differences between Chinese women and women in Western countries and possible mechanisms. Food Science and Human Wellness 2: 146–161.

    Article  Google Scholar 

  • Hernande-Ledesma, B., C.C. Hsieh, and B.O. de Lumen. 2011. Relationship between lunasin’s sequence and its inhibitory activity of histones H3 and H4 acetylation. Molecular Nutrition & Food Research 55: 989–998.

    Article  CAS  Google Scholar 

  • Hsieh, C.C., B. Hernandez-Ledesma, and B.O. de Lumen. 2010. Soybean peptide lunasin suppresses in vitro and in vivo 7,12-dimethylbenz anthracene-induced tumorigenesis. Journal of Food Science 75: H311–H316.

    Article  CAS  PubMed  Google Scholar 

  • Ideker, T., and R. Nussinov. 2017. Network approaches and applications in biology. PLoS Computational Biology 13: e1005771.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Jeong, H.J., J.H. Park, Y. Lam, and B.O. de Lumen. 2003. Characterization of lunasin isolated from soybean. Journal of Agricultural and Food Chemistry 51: 7901–7906.

    Article  CAS  PubMed  Google Scholar 

  • Jeong, J.B., H.J. Jeong, J.H. Park, S.H. Lee, J.R. Lee, H.K. Lee, G.Y. Chung, J.D. Choi, and B.O. de Lumen. 2007. Cancer-preventive peptide lunasin from Solanum nigrum L. inhibits acetylation of core histones H3 and H4 and phosphorylation of retinoblastoma protein (Rb). Journal of Agricultural and Food Chemistry 55: 10707–10713.

    Article  CAS  PubMed  Google Scholar 

  • Kennedy, A.R. 1998. The Bowman-Birk inhibitor from soybeans as an anticarcinogenic agent. The American Journal of Clinical Nutrition 68: 1406–1412.

    Article  Google Scholar 

  • Kim, S.E., H.H. Kim, J.Y. Kim, Y.I. Kang, H.J. Woo, and H.J. Lee. 2000. Anticancer activity of hydrophobic peptides from soy proteins. BioFactors 12: 151–155.

    Article  CAS  PubMed  Google Scholar 

  • Kitts, D., and K. Weiler. 2003. Bioactive proteins and peptides from food sources: Applications of bioprocesses used in isolation and recovery. Current Pharmaceutical Design 9: 1309–1323.

    Article  CAS  PubMed  Google Scholar 

  • Koressaar, T., M. Lepamets, L. Kaplinski, K. Raime, R. Andreson, and M. Remm. 2018. Primer3_masker: Integrating masking of template sequence with primer design software. Bioinformatics 34 (11): 1937–1938.

    Article  CAS  PubMed  Google Scholar 

  • Kump, K.L., P.J. Bradbury, R.J. Wisser, E.S. Buckler, A.R. Belcher, M.A. Oropeza-Rosas, J.C. Zwonitzer, S. Kresovich, M.D. McMullen, D. Ware, P.J. Balint-Kurti, and J.B. Holland. 2011. Genome-wide association study of quantitative resistance to southern leaf blight in the maize nested association mapping population. Nature Genetics 43 (2): 163–168.

    Article  CAS  PubMed  Google Scholar 

  • Lam, Y., A. Galvez, and B.O. de Lumen. 2003. Lunasin suppresses E1Amediated transformation of mammalian cells but does not inhibit growth of immortalized and established cancer cell lines. Nutrition and Cancer 47: 88–94.

    Article  CAS  PubMed  Google Scholar 

  • Li, Y.H., G. Zhou, J. Ma, W. Jiang, L.G. Jin, Z. Zhang, Y. Guo, J. Zhang, Y. Sui, L. Zheng, S.S. Zhang, Q. Zuo, X.H. Shi, Y.F. Li, W.H. Zhang, Y. Hu, G. Kong, H.L. Hong, B. Tan, J. Song, Z.X. Liu, Y. Wang, H. Ruan, C.K.L. Yeung, J. Liu, H. Wang, L.J. Zhang, R.X. Guan, K.J. Wang, W.B. Li, S.Y. Chen, R.Z. Chang, Z. Jiang, S.A. Jackson, R. Li, and L.J. Qiu. 2014. De novo assembly of soybean wild relatives for pan-genome analysis of diversity and agronomic traits. Nature Biotechnology 32 (10): 1045–1052.

    Article  CAS  PubMed  Google Scholar 

  • Liu, J., S.H. Jia, M. Kirberger, and N. Chen. 2014. Lunasin as a promising health-beneficial peptide. European Review for Medical and Pharmacological Sciences 18: 2070–2075.

    CAS  PubMed  Google Scholar 

  • Lohse, M., A. Nagel, T. Herter, P. May, M. Schroda, R. Zrenner, T. Tohge, A.R. Fernie, M. Stitt, and B. Usadel. 2014. Mercator: A fast and simple web server for genome scale functional annotation of plant sequence data. Plant, Cell & Environment 37 (5): 1250–1258.

    Article  CAS  Google Scholar 

  • Losso, J.N. 2008. The biochemical and functional food properties of the bowman-birk inhibitor. Critical Reviews in Food Science and Nutrition 48: 94–118. https://doi.org/10.1080/10408390601177589.

    Article  CAS  PubMed  Google Scholar 

  • Lule, V.K., S. Garg, S.D. Pophaly, Hitesh, and S.D. Tomar. 2015. Potential health benefits of Lunasin: A multifaceted Soy—derived bioactive peptide. Journal of Food Science 80 (3): R485–R494. https://doi.org/10.1111/1750-3841.12786.

    Article  CAS  PubMed  Google Scholar 

  • McCue, P., and K. Shetty. 2004. Health benefits of soy isoflavonoids and strategies for enhancement: A review. Critical Reviews in Food Science and Nutrition 44: 361–367.

    Article  CAS  PubMed  Google Scholar 

  • Messina, M.J., V. Persky, K.D. Setchell, and S. Barnes. 1994. Soy intake and cancer risk: A review of the in vitro and in vivo data. Nutrition and Cancer 21 (2): 113–131.

    Article  CAS  PubMed  Google Scholar 

  • Nakamori, T. 2002. Antiobesity effects of soy proteins and soy peptides. Food Style 21: 86–88.

    Google Scholar 

  • Nakurte, I., I. Kirhnere, J. Namniece, K. Saleniece, L. Krigere, P. Mekss, Z. Vicupe, M. Bleidere, L. Legzdina, and R. Muceniece. 2013. Detection of the lunasin peptide in oats (Avena sativa L.). Journal of Cereal Science 57: e319–e324.

    Article  CAS  Google Scholar 

  • Nordborg, M., and S. Tavare. 2002. Linkage disequilibrium: What history has to tell us. Trends in Genetics 18: 83–90.

    Article  CAS  PubMed  Google Scholar 

  • Nordborg, M., and D. Weigel. 2008. Next-generation genetics in plants. Nature 456: 720–723.

    Article  CAS  PubMed  Google Scholar 

  • Park, J.H., H.J. Jeong, and B.O. de Lumen. 2007. In vitro digestibility of the cancer-preventive soy peptides lunasin and BBI. Journal of Agricultural and Food Chemistry 55: 10703–10706.

    Article  CAS  PubMed  Google Scholar 

  • Pena-Ramos, E.A., and Y.L. Xiong. 2002. Antioxidant activity of soy protein hydrolysates in a liposomal system. Journal of Food Science 67: 2952–2956.

    Article  CAS  Google Scholar 

  • Qi, R.F., Z.W. Song, and C.W. Chi. 2005. Structural features and molecular evolution of Bowman–Birk protease inhibitors and their potential application. Acta Biochimica et Biophysica Sinica 37: 283–292.

    Article  CAS  PubMed  Google Scholar 

  • Revilleza, M.J., A.F. Galvez, D.C. Krenz, and B.O. de Lumen. 1996. An 8 kDa methionine-rich protein from soybean (Glycine max) cotyledon: Identification, purification, and N-terminal sequence. Journal of Agricultural and Food Chemistry 44: 2930–2935.

    Article  CAS  Google Scholar 

  • Sebolt, A.M., R.C. Shoemaker, and B.W. Diers. 2000. Analysis of a Quantitative Trait Locus Allele from Wild Soybean That Increases Seed Protein Concentration in Soybean. Crop Science 40 (5): 1438–1444. https://doi.org/10.2135/cropsci2000.4051438x.

  • Shidal, C., N. Al-Rayyan, K. Yaddanapudi, and K.R. Davis. 2016. Lunasin is a novel therapeutic agent for targeting melanoma cancer stem cells. Oncotarget 7 (51): 84128–84141.

    Article  PubMed  PubMed Central  Google Scholar 

  • Shin, Z., R. Yu, S.A. Park, D.K. Chung, C.W. Ahn, H.S. Nam, K.S. Kim, and H.J. Lee. 2001. His-His-Leu, an angiotensin I converting enzyme inhibitory peptide derived from Korean soybean paste, exerts antihypertensive activity in vivo. Journal of Agricultural and Food Chemistry 49: 3004–3009.

    Article  CAS  PubMed  Google Scholar 

  • Singh, B.P., S. Vij, and S. Hati. 2014. Functional significance of bioactive peptides derived from soybean. Peptides 54: 171–179.

    Article  CAS  PubMed  Google Scholar 

  • Thimm, O., O. Bläsing, Y. Gibon, A. Nagel, S. Meyer, P. Krüger, J. Selbig, L.A. Müller, S.Y. Rhee, and M. Stitt. 2004. MAPMAN: A user-driven tool to display genomics data sets onto diagrams of metabolic pathways and other biological processes. The Plant Journal 37: 914–939. https://doi.org/10.1111/j.1365-313X.2004.02016.x.

    Article  CAS  PubMed  Google Scholar 

  • USDA–NASS. 2015a. Crop production: 2014 summary. Washington, DC: USDA National Agricultural Statistics Service. http://www.nass.usda.gov.

    Google Scholar 

  • ———. 2015b. Crop values: 2014 summary. Washington, DC: USDA National Agricultural Statistics Service. http://www.nass.usda.gov.

    Google Scholar 

  • Velasquez, M.T., and S.J. Bhathena. 2007. Role of dietary soy protein in obesity. International Journal of Medical Sciences 4: 72–82.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Wan, X., H. Liu, Y. Sun, J. Zhang, X. Chen, and N. Chen. 2017. Lunasin: A promising polypeptide for the prevention and treatment of cancer (Review). Oncology Letters 13 (6): 3997–4001. https://doi.org/10.3892/ol.2017.6017.

  • Wang, W., V.P. Dia, M. Vasconez, E.D. de Mejia, and R. Nelson. 2008. Analysis of soybean protein-derived peptides and the effect of cultivar, environmental conditions, and processing on lunasin concentration in soybean and soy products. Journal of AOAC International 91: 936–946.

    Article  CAS  PubMed  Google Scholar 

  • Wen, Z., R. Tan, J. Yuan, C. Bales, W. Du, S. Zhang, M.I. Chilvers, C. Schmidt, Q. Song, P.B. Cregan, and D. Wang. 2014. Genome-wide association mapping of quantitative resistance to sudden death syndrome in soybean. BMC Genomics 15: 809. http://www.biomedcentral.com/1471-2164/15/809.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Wen, Z., R. Tan, S. Zhang, P. Collins, J. Yuan, W. Du Jiazheng, C. Gu, S. Ou, Q. Song, Y.Q.C. An, J.F. Boyse, M.I. Chilvers, and D. Wang. 2018. Integrating GWAS and gene expression data for functional characterization of resistance to white mold in soya bean. Plant Biotechnology Journal 16 (11): 1825–1835.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Xu, H., J. Abe, Y. Gai, and Y. Shimamoto. 2002. Diversity of chloroplast DNA SSRs in wild and cultivated soybeans: Evidence for multiple origins of cultivated soybean. Theoretical and Applied Genetics 105 (5): 645–653.

    Article  CAS  PubMed  Google Scholar 

  • Yuan, J., U. Rosyara, Z. Wen, M. Lague, and B. Bizimungu. 2019. GWAS analysis of potato (Solanum tuberosum L.) resistance to common scab in Canadian potato germplasm. Potato Research. https://doi.org/10.1007/s11540-019-09437-w.

Download references

Author information

Authors and Affiliations

  1. Plant Genomics and Biotechnology Lab, Department of Biological and Forensic Sciences, Fayetteville State University, Fayetteville, NC, USA

    Jiazheng Yuan, Meriam Bousselham & Moulay Abdelmajid Kassem

Authors
  1. Jiazheng Yuan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Meriam Bousselham
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Moulay Abdelmajid Kassem
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Moulay Abdelmajid Kassem .

Editor information

Editors and Affiliations

  1. Department of Biological and Forensic Sciences, Fayetteville State University, Fayetteville, NC, USA

    Moulay Abdelmajid Kassem

Rights and permissions

Reprints and permissions

Copyright information

© 2021 The Author(s), under exclusive license to Springer Nature Switzerland AG

About this chapter

Check for updates. Verify currency and authenticity via CrossMark

Cite this chapter

Yuan, J., Bousselham, M., Kassem, M.A. (2021). Bioactive Anticancer Peptides in Soybean Seeds. In: Kassem, M.A. (eds) Soybean Seed Composition. Springer, Cham. https://doi.org/10.1007/978-3-030-82906-3_11

Download citation

Publish with us

Policies and ethics

Search

Navigation