Abstract
In pathologies of the esophagus such as esophageal atresia, cancers and caustic injuries, methods for full thickness esophageal replacement require the sacrifice of healthy intra-abdominal organs such as the stomach and the colon. These methods are associated with high morbidity, mortality and poor functional results. The reconstruction of an esophageal segment by tissue engineering (TE) could answer this problem. For esophageal TE, this approach has been explored mainly by a combination of matrices and cells. In this chapter, we will discuss the studies on full organ esophageal decellularization, including the animal models, the methods of decellularization and recellularization.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Arakelian L, Caille C, Faivre L, Corté L, Bruneval P, Shamdani S et al (2019) A clinical-grade acellular matrix for esophageal replacement. J Tissue Eng Regen Med. https://doi.org/10.1002/term.2983
Asnaghi MA, Jungebluth P, Raimondi MT, Dickinson SC, Rees LEN, Go T et al (2009) A double-chamber rotating bioreactor for the development of tissue-engineered hollow organs: From concept to clinical trial. Biomaterials 30(29):5260–5269
Badylak SF, Vorp DA, Spievack AR, Simmons-Byrd A, Hanke J, Freytes DO et al (2005) Esophageal reconstruction with ECM and muscle tissue in a dog model. J Surg Res
Badylak SF, Hoppo T, Nieponice A, Gilbert TW, Davison JM, Jobe BA (2011) Esophageal preservation in five male patients after endoscopic inner-layer circumferential resection in the setting of superficial cancer: a regenerative medicine approach with a biologic scaffold. Tissue Eng Part A [Internet] 17(11–12):1643–50. Available from: http://www.liebertonline.com/doi/abs/https://doi.org/10.1089/ten.tea.2010.0739
Barron MR, Blanco EW, Aho JM, Chakroff J, Johnson J, Cassivi SD et al (2016) Full-thickness oesophageal regeneration in pig using a polyurethane mucosal cell seeded graft. J Tissue Eng Regen Med 12(1):175–185
Bozuk MI, Fearing NM, Leggett PL (2006) Use of decellularized human skin to repair esophageal anastomotic leak in humans. JSLS 10:83–85
Catry J, Luong-Nguyen M, Arakelian L, Poghosyan T, Bruneval P, Domet T et al (2017) Circumferential esophageal replacement by a tissue-engineered substitute using mesenchymal stem cells. Cell Transplant [Internet] 26(12):1831–1839. Available from: http://journals.sagepub.com/doi/https://doi.org/10.1177/0963689717741498
Chang S, Lamm SH (2003) Human health effects of sodium azide exposure: a literature review and analysis. Int J Toxicol
Crapo PM, Gilbert TW, Badylak SF (2011) An overview of tissue and whole organ decellularization processes. Biomaterials 32:3233–3243
Hass R, Kasper C, Böhm S, Jacobs R (2011) Different populations and sources of human mesenchymal stem cells ( MSC ): a comparison of adult and neonatal tissue-derived MSC. Cell Commun Signal 9(1):12
Jensen T, Wanczyk H, Sharma I, Mitchell A, Sayej WN, Finck C (2018) Polyurethane scaffolds seeded with autologous cells can regenerate long esophageal gaps: an esophageal atresia treatment model. J Pediatr Surg
Kjellén L, Lindahl U (2018) Specificity of glycosaminoglycan–protein interactions. Curr Opin Struct Biol 50:101–108
Koch H, Graneist C, Emmrich F, Till H, Metzger R, Aupperle H et al (2012) Xenogenic esophagus scaffolds fixed with several agents: Comparative in vivo study of rejection and inflammation. J Biomed Biotechnol
Kuo B, Urma D (2006) Esophagus—anatomy and development. GI Motil online [Internet], pp 1–20. Available from: http://www.nature.com/gimo/contents/pt1/full/gimo6.html
La Francesca S, Aho JM, Barron MR, Blanco EW, Soliman S, Kalenjian L et al (2018) Long-term regeneration and remodeling of the pig esophagus after circumferential resection using a retrievable synthetic scaffold carrying autologous cells. Sci Rep [Internet] 8(1):4123. Available from: http://www.nature.com/articles/s41598-018-22401-x
Luc G, Charles G, Gronnier C, Cabau M, Kalisky C, Meulle M et al (2018) Decellularized and matured esophageal scaffold for circumferential esophagus replacement: proof of concept in a pig model. Biomaterials 175:1–18
Lucas AD, Merritt K, Hitchins VM, Woods TO, Mcnamee SG, Lyle DB et al (2017) Residual ethylene oxide in medical devices and device material residual ethylene oxide in medical devices and device material
Mallis P, Chachlaki P, Katsimpoulas M, Stavropoulos-Giokas C, Michalopoulos E (2019) Optimization of decellularization procedure in rat esophagus for possible development of a tissue engineered construct. Bioengineering
Marzaro M, Vigolo S, Oselladore B, Conconi MT, Ribatti D, Giuliani S et al (2006) In vitro and in vivo proposal of an artificial esophagus. J Biomed Mater Res A 77(4):795–801
Nakase Y, Nakamura T, Kin S, Nakashima S, Yoshikawa T, Kuriu Y et al (2008) Intrathoracic esophageal replacement by in situ tissue-engineered esophagus. J Thorac Cardiovasc Surg 136(4):850–859
Ozeki M, Narita Y, Kagami H, Ohmiya N, Itoh A, Hirooka Y et al (2006) Evaluation of decellularized esophagus as a scaffold for cultured esophageal epithelial cells. J Biomed Mater Res Part A
Poghosyan T, Gaujoux S, Chirica M, Munoz-Bongrand N, Sarfati E, Cattan P (2011) Functional disorders and quality of life after esophagectomy and gastric tube reconstruction for cancer. J Visc Surg [Internet] 148:e327—e335. Available from: http://ac.els-cdn.com.gate2.inist.fr/S1878788611001093/1-s2.0-S1878788611001093-main.pdf?_tid=102588d6-8322-11e7-8a8b-00000aacb35f&acdnat=1502956978_4c9f951fb448fd427801b6ae238e4cf9
Poghosyan T, Gaujoux S, Vanneaux V, Bruneval P, Domet T, Lecourt S et al (2013) In vitro development and characterization of a tissue-engineered Conduit\nResembling esophageal wall using human and pig skeletal myoblast, Oral\nEpithelial Cells, and biologic scaffolds. Tissue Eng Part a 19(19–20):2242–2252
Poghosyan T, Sfeir R, Michaud L, Bruneval P, Domet T, Vanneaux V et al (2015) Circumferential esophageal replacement using a tube-shaped tissue-engineered substitute: an experimental study in minipigs. Surg (United States) [Internet] 158(1):266–277. https://doi.org/10.1016/j.surg.2015.01.020
Reing JE, Zhang L, Myers-irvin J, Ph D, Cordero KE, Freytes DO et al (2009) Degradation products of extracellular matrix affect cell migration and proliferation. 15(3)
Scheffler SU, Cmd JG, Cmd JK, Przybilla D (2008) Remodeling of ACL allografts is inhibited by peracetic acid sterilization. Clin Orthop Relat Res 466:1810–1818
Seery JP (2002) Stem cells of the oesophageal epithelium. J Cell Sci 115:783–1789
Takeoka Y, Matsumoto K, Taniguchi D, Tsuchiya T, Machino R, Moriyama M et al (2019) Regeneration of esophagus using a scaffoldfree biomimetic structure created with biothree-dimensional printing. PLoS ONE 14(3):1–12
Tan B, Wei RQ, Tan MY, Luo JC, Deng L, Chen XH et al (2013) Tissue engineered esophagus by mesenchymal stem cell seeding for esophageal repair in a canine model. J Surg Res 182(1):40–48
Totonelli G, Maghsoudlou P, Georgiades F, Garriboli M, Koshy K, Turmaine M et al (2013) Detergent enzymatic treatment for the development of a natural acellular matrix for oesophageal regeneration. Pediatr Surg Int
Urbani L, Camilli C, Phylactopoulos D, Crowley C, Natarajan D, Scottoni F et al (2018) Multi-stage bioengineering of a layered oesophagus with in vitro expanded muscle and epithelial adult progenitors. Nat Commun
Witt TA, Marler RJ, Pislaru SV, Robert D (2016) Low-dose gamma irradiation of decellularized heart valves results in tissue injury in vitro and in vivo. Ann Thorac Surg 101(2):667–674
Xiuunl I, Xiaoz UO, Weiz HI, Pengy A, Huiix IE, Zhiingy A (2009) Grafts of porcine small intestinal submucosa with cultured autologous oral mucosal epithelial cells for esophageal repair in a canine model. Exp Biol Med (Maywood) 234(4):453–461
Yamaguchi N, Isomoto H, Kobayashi S, Kanai N, Kanetaka K, Sakai Y et al (2017) Oral epithelial cell sheets engraftment for esophageal strictures after endoscopic submucosal dissection of squamous cell carcinoma and airplane transportation. Sci Rep 7(1)
Ziegler A, Gonzalez L, Blikslager A (2016) Large animal models: the key to translational discovery in digestive disease research. Cmgh [Internet] 2(6):716–24. https://doi.org/10.1016/j.jcmgh.2016.09.003
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2021 Springer Nature Switzerland AG
About this chapter
Cite this chapter
Arakelian, L., Godefroy, W., Faivre, L., Cattan, P. (2021). Esophagus Decellularization. In: Kajbafzadeh, AM. (eds) Decellularization Methods of Tissue and Whole Organ in Tissue Engineering. Advances in Experimental Medicine and Biology, vol 1345. Springer, Cham. https://doi.org/10.1007/978-3-030-82735-9_2
Download citation
DOI: https://doi.org/10.1007/978-3-030-82735-9_2
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-030-82734-2
Online ISBN: 978-3-030-82735-9
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)