Abstract
The study of supramolecular structures in biological systems aiming at the investigation of mechanisms of interaction, detection, kinetics, among others, is of great relevance. Due to the nanometric dimensions of such structures, scattering techniques are widely used for their characterization, in addition to allowing great versatility with regard to the physical state of the sample to be analyzed, which includes liquids, gels, gases, and solids. In this chapter, we will present an overview of DLS, SAXS, SANS, and SLS scattering techniques, as well as a brief explanation on what structural parameters could be obtained from each of them. In the following, we present application examples involving different classes of biomolecules such as proteins, DNAs, lipids, and polymers, where the SAXS technique was used as the main tool for their structural characterization.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Andersen CBF, Torvund-Jensen M, Nielsen MJ, Oliveira CLP, Hersleth HP, Andersen NH, Pedersen JS, Andersen GR, Moestrup SK (2012) Nature 489:456–459
Caille A (1972) Comptes Rendus Hebdomadaires Des Seances De L Academie Des Sciences Serie B 274:891
Capito RM, Azevedo HS, Velichko YS, Mata A, Stupp SI (2008) Science 319:1812–1818
Chu B, Liu TB (2000) J Nanopart Res 2:29–41
Chuaychob S, Thammakhet-Buranachai C, Kanatharana P, Thavarungkul P, Buranachai C, Fujita M, Maeda M (2020) Anal Methods 12:230–238
Ewert K, Evans H, Zidovska A, Bouxsein N, Ahmad A, Safinya CR (2006) J Am Chem Soc 128:3998–4006
Folkertsma L, Zhang KH, Czakkel O, Boer HL, Hempenius MA, van den Berg A, Odijk M, Vancso GJ (2017) Macromolecules 50:296–302
Gerbelli BB, Rubim RL, Silva ER, Nallet F, Navailles L, Oliveira CLP, Oliveira EA (2013) Langmuir 29:13717–13722
Gerbelli BB, Silva ER, Soares BM, Alves WA, Oliveira EA (2018) Langmuir 34:2171–2179
Glatter O (1977) J Appl Cryst 10:415–421
Goni F (1838) BBA-Biomembranes 2014:1467–1476
Heller WT (2010) Acta Crystallogr Sect D Biol Crystallogr 66:1213–1217
Jacrot B (1976) Rep Prog Phys 39:911–953
Jones AR (1999) Prog Energy Combust Sci 25:1–53
Koltover I, Salditt T, Radler J, Safinya CR (1998) Science 281:78–81
Lendermann J, Winter R (2003) Phys Chem Chem Phys 5:1440–1450
Lieberthal W, Triaca V, Levine J (1996) Am J Physiol Ren Fluid Electrolyte Physiol 270:F700–F708
Lombardo D, Calandra P, Kiselev MA (2020) Molecules 25:5624–5661
Luzzati V, Mustacchi H, Skoulios A (1957) Nature 180:600–601
Mahieu E, Gabel F (2018) Acta Crystallogr Sect D Struct Biol 74:715–726
Mertens HD, Piljic A, Schultz C, Syergun DI (2012) Biophys J 102:2866–2875
Miller CC (1924) Proceedings of the Royal Society of London Series A, containing papers of a mathematical and physical character, vol 106, pp 724–749
Nasta V, Vela S, Gourdoupis S, Ciofi-Baffoni S, Svergun DI, Banci L (2019) Sci Rep 9:18986
Oliveira CLP (2011) Current trends in X-ray crystallography. InTech, London, pp 367–392
Oliveira CLP, Behrens MA, Pedersen JS, Erlacher K, Otzen D (2009) J Mol Biol 387:147–161
Oliveira CLP, Juul S, Jorgensen HL, Knudsen B, Tordrup D, Oteri F, Falconi M, Koch J, Desideri A, Pedersen JS, Andersen FF, Knudsen BR (2010) ACS Nano 4:1367–1376
Oliveira CLP, Gerbelli BB, Silva ER, Nallet F, Navailles L, Oliveira EA, Pedersen JS (2012) J Appl Cryst 45:1278–1286
Orthaber D, Bergmann A, Glatter O (2000) J Appl Cryst 33:218–225
Pabst G, Danner S, Podgornik R, Katsaras J (2007) Langmuir 23:11705–11711
Petoukhov MV, Svergun DI (2006) Eur Biophys J Biophys Lett 35:567–576
Piljic A, Schultz C (2006) Mol Biol Cell 17:3318–3328
Radler JO, Koltover I, Salditt T, Safinya CR (1997) Science 275:810–814
Rahimabadi PS, Khodaei M, Koswattage KR (2020) X-Ray Spectrom 49:348–373
Rambo RP, Tainer JA (2013) Annu Rev Biophys 42:415–441
Roig AR, Alessandrini JL (2007) Part Part Syst Charact 23:431–437
Rojas J, Gerbelli BB, Ribeiro A, Nantes-Cardoso I, Giuntini F, Alves WA (2019) Biopolymers 110:e23245
Sarode HN, Yang Y, Motz AR, Li YR, Knauss DM, Seifert S, Herring AM (2017) J Phys Chem C 121:2035–2045
Scotti A, Liu W, Hyatt JS, Herman ES, Choi HS, Kim JW, Lyon LA, Gasser U, Fernandez-Nieves A (2015) J Chem Phys 142:234905–234918
Silva ER, Oliveira EA, Fevrier A, Nallet F, Navailles L (2011) Eur Phys J E 34:83
Silva ER, Listik E, Han SW, Alves WA, Soares BM, Reza M, Ruokolainen J, Hamley IW (2018) Biophys Chem 233:1–12
Singer S, Nicolson G (1972) Science 175:720
Svergun DI, Koch MHJ (2003) Rep Prog Phys 66:1735–1782
Talebian E, Talebian M (2013) Optik 124:2324–2326
Xu RL (2015) Particuology 18:11–21
Zemb T, Lindner P (2002) Neutrons, X-rays and light: scattering methods applied to soft condensed matter. Elsevier, Amsterdam
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2022 The Author(s), under exclusive license to Springer Nature Switzerland AG
About this chapter
Cite this chapter
Gerbelli, B.B., Oliveira, C.L.P. (2022). Biological Systems Investigated by Small-Angle X-Ray Scattering. In: Kubota, L.T., da Silva, J.A.F., Sena, M.M., Alves, W.A. (eds) Tools and Trends in Bioanalytical Chemistry. Springer, Cham. https://doi.org/10.1007/978-3-030-82381-8_7
Download citation
DOI: https://doi.org/10.1007/978-3-030-82381-8_7
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-030-82380-1
Online ISBN: 978-3-030-82381-8
eBook Packages: Chemistry and Materials ScienceChemistry and Material Science (R0)