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Smart Polymer-Based Multicomponent Nanosystem for Enhanced Anticancer Photodynamic Therapy

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Soft Matter Systems for Biomedical Applications

Part of the book series: Springer Proceedings in Physics ((SPPHY,volume 266))

Abstract

Nanotechnology is a promising approach for creation of polymer-based nanocomposite dye for Photodynamic therapy (PDT). Polymer molecules can be preferentially accumulated in tumor die, prevent photosensitizer aggregation. Thermosensitive star-like Dextran-graft-Poly-N-isopropylacrylamide copolymer (D-PNIPAM) was used as matrix for creation of multicomponent nanosystems for PDT. Au nanoparticles (AuNPs) and photosensitizer Chlorin e6 (Ce6) were incorporated into polymer. The behavior of nanosystems was studied by optical absorption, dynamic light scattering in water and in Hank’s buffer solution within temperature range of 25–40 °C. No drastic changes accompanied by aggregation process in the nanosystem in Hank’s saline solution in comparison with one prepared in water were registered. In vitro examination of the PDT activity of the prepared nanosystem on the culture of MT-4 human malignant lymphocytes revealed the death of 40% lymphocytes preincubated with nanocomposite.

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Abbreviations

DLS:

Dynamic Light Scattering

HPLC:

High-performance Liquid Chromatography

LCST:

Lower critical solution temperature

PDT:

Photodynamic therapy

RPMI:

Roswell Park Memorial Institute medium

SEC:

Size Exclusion Chromatography

SPR:

Surface plasmon resonance

TDA:

Triple Detector Array

TEM:

Transmission Electron Microscopy

UV:

Ultraviolet

AuNPs:

Au nanoparticles, gold nanoparticles

Ce6:

Chlorin e6

DMSO:

Dimethyl sulfoxide

D-PNIPAM:

Dextran-graft-Poly(N-isopropylacrylamide)

NIPAM:

N-isopropylacrylamide

PNIPAM:

Poly(N-isopropylacrylamide)

PS:

Photosensitizer

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Acknowledgements

The authors would like to thank I. Shton and E. Shishko from R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology for in vitro experiment. The publication is based on the research provided partially by the grant support of the Ministry of the Education and Science of Ukraine, the Belarusian Republican Foundation for basic research—joint Ukrainian-Belarusian research and development projects “Design and physico-chemical properties of novel multicomponent nanosystems for the treatment and diagnostics of solid tumors” (2019–2020), and by National Research Foundation of Ukraine. Project 2020.02/0022 “Plasmon hybrid nanosystems “metal-polymer-fluorophore” with enhanced optical response for photonics and biomedical applications”.

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Authors and Affiliations

  1. Faculty of Chemistry, Taras Shevchenko National University, Volodymyrska Street 60, Kyiv, 01601, Ukraine

    Nataliya Kutsevol & Yuliia Kuziv

  2. Faculty of Physics, Taras Shevchenko National University, Volodymyrska Street 60, Kyiv, 01601, Ukraine

    Leonid Bulavin

  3. R.E. Kavetsky Institute for Experimental Pathology Oncology and Radiobiology, Vasilkivska Street 45, Kyiv, 03022, Ukraine

    Vasyl Chekhun

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  1. Nataliya Kutsevol
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Editors and Affiliations

  1. Taras Shevchenko National University of Kyiv, Kyiv, Ukraine

    Leonid Bulavin

  2. National Academy of Science of Ukraine, Biocolloidal Chemistry Institute named after F.D. Ovharenko, Kyiv, Ukraine

    Nikolai Lebovka

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Kutsevol, N., Kuziv, Y., Bulavin, L., Chekhun, V. (2022). Smart Polymer-Based Multicomponent Nanosystem for Enhanced Anticancer Photodynamic Therapy. In: Bulavin, L., Lebovka, N. (eds) Soft Matter Systems for Biomedical Applications. Springer Proceedings in Physics, vol 266. Springer, Cham. https://doi.org/10.1007/978-3-030-80924-9_14

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