Abstract
Phospholipid fatty acid (PLFA) analysis is used to measure the microbial biomass and the phospholipids present in the environmental samples. Microalgae spirulina is found to be a rich source of very-long-chain polyunsaturated fatty acids (VLCPUFAs) and has been used as a neutraceutical and regenerative medicine in the biotechnological industries as PUFAs are not synthesized in the human body due to the lack of enzymes for their bioconversion and must be supplied through the diet. Eicosapentanoic acid (EPA) and docosahexanoic acid (DHA) are the two most important long-chain omega-3 (ω-3) polyunsaturated fatty acids involved in the human physiology, and their precursors stearic acid (ω-9), linoleic acid (ω-6), and gamma linolenic acid (ω-6) were found to be in higher concentrations in Spirulina platensis. GC or GC-MS is used to analyze the presence of PLFA in the sample. The PLFA analysis was carried to detect the presence of polyunsaturated fatty acids in the Spirulina platensis which are the essential components in the diet of humans. The analysis involves overnight drying of the sample and followed by Bligh-Dyer lipid extraction. The obtained extract is dried and dissolved in chloroform and loaded onto a 96-well solid phase extraction plate. The eluted phospholipids are dried and transesterified. The resulting fatty acid methyl esters are analyzed by GC and quantified relative to an internal standard.
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References
Brown MR et al (1997) Nutritional properties of microalgae for mariculture. Aquaculture 151(1–4):315–331. https://doi.org/10.1016/s0044-8486(96)01501-3
Adarme-Vega T et al (2012) Microalgal biofactories: a promising approach towards sustainable omega-3 fatty acid production. Microb Cell Factories 11(1):96
Martins D et al (2013) Alternative sources of n-3 long-chain polyunsaturated fatty acids in marine microalgae. Mar Drugs 11(7):2259–2281
Ciferri O, Tiboni O (1985) The biochemistry and industrial potential of spirulina. Annu Rev Microbiol 39(1):503–526
Sotiroudis T, Sotiroudis G (2013) Health aspects of Spirulina (Arthrospira) microalga food supplement. J Serb Chem Soc 78(3):395–405
Sasson A (1997) Micro biotechnologies: recent developments and prospects for developing countries. BIOTEC Publication 1/2542, pp 11–31
Burdge GC, Calder PC (2005) Conversion of α-linolenic acid to longer-chain polyunsaturated fatty acids in human adults. Reprod Nutr Dev 45(5):581–597
Ryckebosch E et al (2011) Optimization of an analytical procedure for extraction of lipids from microalgae. J Am Oil Chem Soc 89(2):189–198
Gester H (1998) Can adults adequately convert alpha-linolenic acid (18:3n-3) to eicosapentaenoic acid (20:5n-3) and docosahexaenoic acid (22:6n-3)? Int J Vitam Nutr Res 68:159–173
Keen H et al (1993) Treatment of diabetic neuropathy with -linolenic acid. Diabetes Care 16(1):8–15
Johnson M et al (2009) Omega-3/Omega-6 fatty acids for attention deficit hyperactivity disorder. J Atten Disord 12(5):394–401
Gurr, M.I. & Harwood, J.L. & Frayn, Keith. (2002). Lipid Biochemistry An Introduction. 10.1007/978-94-011-3062-2.
Voss A et al (1992) Differences in the interconversion between 20- and 22-carbon (n − 3 and (n − 6) polyunsaturated fatty acids in rat liver. Biochim Biophys Acta (BBA): Lipids Lipid Metab 1127(1):33–40
Brooks D (1981) Modern nutrition in health and disease, 6th Edition Edited by Robert S. Goodhart and Maurice E. Shils. Philadelphia: Lea and Febiger, 1980. 1370 pages. $47.50, Hardcover. J Nurse Midwifery 26(6):36–37
Handayania NA, Ariyantib D (2012) Potential production of polyunsaturated fatty acids from microalgae. J Bioprocess Biotechn 01(S1)
Watanabe H et al (2003) Anandamide and arachidonic acid use epoxyeicosatrienoic acids to activate TRPV4 channels. Nature 424(6947):434–438
Rapoport SI (2008) Arachidonic acid and the brain. J Nutr 138(12):2515–2520
Grey A, Bolland M (2014) Clinical trial evidence and use of fish oil supplements. JAMA Intern Med 174(3):460
Simopoulos AP (2002) The importance of the ratio of omega-6/omega-3 essential fatty acids. Biomed Pharmacother 56(8):365–379
Wood PL (2012) Lipidomics of Alzheimer’s disease: current status. Alzheimer’s Res Ther 4(1):5
Astarita G et al (2010) Deficient liver biosynthesis of docosahexaenoic acid correlates with cognitive impairment in Alzheimer’s disease. PLoS One 5(9):e12538
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Sara, L., Konda, S., Nikitha, B., Palupanuri, N. (2021). Phospholipid Fatty Acid Profile of Spirulina platensis. In: Vlamos, P. (eds) GeNeDis 2020. Advances in Experimental Medicine and Biology, vol 1339. Springer, Cham. https://doi.org/10.1007/978-3-030-78787-5_21
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DOI: https://doi.org/10.1007/978-3-030-78787-5_21
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