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Adiponectin and Its Effects on Acute Leukemia Cells: An Experimental and Bioinformatics Approach

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GeNeDis 2020

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 1338))

Abstract

Acute lymphoblastic leukemia (ALL) is the most common pediatric malignancy. It is known that deregulation of adipokine pathways is probably implicated in the ontogenesis of ALL. The present work aims at investigating the role of adiponectin and its effects on an ALL cell line. The CCRF-CEM cells were used as a model. Cells have been treated with adiponectin, with different concentrations up to 72 h. Cytotoxicity and cell cycle distribution were investigated for all concentrations using flow cytometry. Selected concentrations were also used for additional microarray analysis, using a small gene set of cancer-related genes. Lower and higher adiponectin concentrations did not produce an inhibition of proliferation, as well as an increase in cell death. It was found that adiponectin regulated differentially genes, such as CD22, CDH1, IFNG, LCK, MSH2, SPINT2, and others. At the same time, it appeared that adiponectin-related gene expression was more active on chromosomes 18 and 1. Machine learning classification algorithms showed that several genes were grouped together indicating common regulatory mechanisms. The present study showed that adiponectin is able to induce gene differential expression in leukemic cells in vitro, suggesting a possible role in the progression of leukemia. It is also an indication that more studies are required in order to further understand the role of adiponectin and adipokines in general in the role of human neoplasms.

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Author information

Authors and Affiliations

  1. Naval Hospital of Athens, Department of Endocrinology Diabetes and Metabolism, Athens, Greece

    Athanasios N. Tsartsalis

  2. First Department of Dermatology and Venereology, “Andreas Syggros” Hospital National and Kapodistrian University of Athens, Athens, Greece

    Anna Tagka

  3. National Technical University of Athens, School of Electrical and Computer Engineering Biomedical Engineering Laboratory, Athens, Greece

    Athanasios Kotoulas

  4. First Propaedeutic and Internal Medicine Department, Aristotelian University if Thessaloniki, General Hospital of Thessaloniki, Thessaloniki, Greece

    Daphne Mirkopoulou

  5. Biomedical Research Foundation of Academy of Athens, Clinical, Translational, Experimental Surgery Research Centerment of Pediatrics, Athens, Greece

    Styliani A. Geronikolou

  6. National and Kapodistrian University of Athens, First Department of Pediatrics, Choremeio Research Laboratory, Athens, Greece

    Lambrou G

Authors
  1. Athanasios N. Tsartsalis
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  2. Anna Tagka
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  3. Athanasios Kotoulas
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  4. Daphne Mirkopoulou
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  5. Styliani A. Geronikolou
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  6. Lambrou G
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Corresponding author

Correspondence to Lambrou G .

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Editors and Affiliations

  1. Department of Informatics, Ionian University, Corfu, Greece

    Panayiotis Vlamos

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Tsartsalis, A.N., Tagka, A., Kotoulas, A., Mirkopoulou, D., Geronikolou, S.A., G, L. (2021). Adiponectin and Its Effects on Acute Leukemia Cells: An Experimental and Bioinformatics Approach. In: Vlamos, P. (eds) GeNeDis 2020. Advances in Experimental Medicine and Biology, vol 1338. Springer, Cham. https://doi.org/10.1007/978-3-030-78775-2_14

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