Determination of Structure and Anticancer Activity of MM₁ Compound Isolated from Endophytic Fungus Aspergillus terreus-RTN3 of Alpinia chinensis Rosc.

Abstract

Alpinia chinensis Rosc. was widely used as spices and medicines. Leaves, stems and rhizomes of the plant contain essential oils, provide a useful habitat for endophytic fungi groups differently. This study has isolated endogenous A. terreus—RTN3 strain from young stems of Alpinia chinensis Rosc.-Zingiberaceae family. Cultivating and extracting compounds containing metabolites that are resistant to Staphylococcus aureus and Methicillin-resistant Staphylococcus aureus (MRSA). Determining the number of antibacterial compounds in the crude extract by thin-layer chromatography and vacuum liquid chromatography. The research has isolated pure MM₁ compound with high antioxidant activity and spectral resolution to find the structure compound MM₁ (methyl 2-acetyl-5-methoxy-4-oxo-4H-chromen-7-carboxylate). This is a new compound that has not been announced in any works before. MM₁ compound has a high antioxidant activity, the ability to catch α,α-diphenyl-β-picrylhydrazyl (DPPH) free radicals increases linearly by concentration, at concentration 400 ppm of MM₁ is capable of capturing over 80% of DPPH free radicals. The results showed that MM₁ compound's ability to inhibit cancer cell lines increased linearly, at concentrations of 100 µg/mL of MM₁ compound, which inhibited nearly 80% of cancer cell lines and was highest in liver cancer cell line HepG2 (inhibited 86.82%). The MM₁ compound has also been shown to inhibit cancer cells through the ability to induce apoptosis on the NCI-H460 lung cancer cell line under the investigated conditions.