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The Influence of High D-glucose Concentrations on Increasing the Expression of EGR-1, PTEN and GGPS-1 Involved in Insulin Resistance of AT-MSCs

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8th International Conference on the Development of Biomedical Engineering in Vietnam (BME 2020)

Part of the book series: IFMBE Proceedings ((IFMBE,volume 85))

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Abstract

Type 2 diabetes mellitus (T2DM) is increasing worldwide, characterized by insulin resistance and hyperglycemia. The results of previous studies have demonstrated that high D-glucose concentrations alter the characteristic and function of adipose tissue-derived mesenchymal stem cells (AT-MSCs). Besides, early growth response factor-1 (EGR-1) and insulin resistance mediators (PTEN and GGPS-1) were highly upregulated in diabetic AT-MSCs (dAT-MSCs) compared with non-diabetic AT-MSCs (nAT-MSCs). In this research, we examined the effect of high glucose concentrations on nAT-MSCs in comparison to dAT-MSCs on the expression of EGR-1, PTEN, and GGPS-1 involved in insulin resistance of human AT-MSCs. The expression of insulin resistance-related genes and EGR-1 protein were analyzed by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and Western blot, respectively. The results show that the high D-Glucose concentrations (25, 50, and 100 mM) enhanced the expression of EGR-1 and insulin resistance-related genes in nAT-MSCs compared with non-treated nAT-MSCs and dAT-MSCs. Notably, the increase of high D-Glucose concentration remarkably upregulated the expression of EGR-1, PTEN, and GGPS-1 in both nAT-MSCs and dAT-MSCs. The effect of high D-glucose concentration (100 mM) remarkably increased the expression of EGR-1, PTEN, and GGPS-1 in human AT-MSCs. The results of this study will expand our knowledge about the impact of high glucose concentration on insulin resistance in human AT-MSCs for the improvement in diabetic treatment.

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Abbreviations

EGR-1 :

Early growth response-1

PTEN :

Phosphatase and tensin homologue

GGPS-1 :

Geranylgeranyl diphosphate synthase-1

IRS-1 :

Insulin receptor substrate-1

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Acknowledgements

The authors thank to the financial support by the grant from the Biotechnology Center of Ho Chi Minh City (DV01/17-19). We also thank professor Osamu Ohneda for providing stem cells.

Conflict of Interest

The authors declare no conflict of interest.

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Authors and Affiliations

  1. Biotechnology Center of Ho Chi Minh City, Ho Chi Minh City, 700000, Vietnam

    Huu-Phuong Mai, Nguyen Trong Binh, Dang-Quan Nguyen & Hoa-Xo Duong

  2. International University, Ho Chi Minh City, 700000, Vietnam

    Nhu-Thuy Trinh & Vong Binh Long

  3. Vietnam National University of Ho Chi Minh City, Ho Chi Minh City, 700000, Vietnam

    Nhu-Thuy Trinh & Vong Binh Long

Authors
  1. Huu-Phuong Mai
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  2. Nhu-Thuy Trinh
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  3. Vong Binh Long
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  4. Nguyen Trong Binh
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  5. Dang-Quan Nguyen
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  6. Hoa-Xo Duong
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Corresponding author

Correspondence to Nhu-Thuy Trinh .

Editor information

Editors and Affiliations

  1. School of Biomedical Engineering, International University—Vietnam National Universities, Ho Chi Minh, Vietnam

    Vo Van Toi

  2. School of Biomedical Engineering, International University—Vietnam National Universities, Ho Chi Minh, Vietnam

    Thi-Hiep Nguyen

  3. School of Biomedical Engineering, International University—Vietnam National Universities, Ho Chi Minh, Vietnam

    Vong Binh Long

  4. School of Biomedical Engineering, International University—Vietnam National Universities, Ho Chi Minh, Vietnam

    Ha Thi Thanh Huong

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Mai, HP., Trinh, NT., Long, V.B., Binh, N.T., Nguyen, DQ., Duong, HX. (2022). The Influence of High D-glucose Concentrations on Increasing the Expression of EGR-1, PTEN and GGPS-1 Involved in Insulin Resistance of AT-MSCs. In: Van Toi, V., Nguyen, TH., Long, V.B., Huong, H.T.T. (eds) 8th International Conference on the Development of Biomedical Engineering in Vietnam. BME 2020. IFMBE Proceedings, vol 85. Springer, Cham. https://doi.org/10.1007/978-3-030-75506-5_42

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  • DOI: https://doi.org/10.1007/978-3-030-75506-5_42

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  • Online ISBN: 978-3-030-75506-5

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