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Fabrication of Injectable Hydrogel from Decellularized Adipose Tissue

  • Conference paper
  • First Online:
8th International Conference on the Development of Biomedical Engineering in Vietnam (BME 2020)

Part of the book series: IFMBE Proceedings ((IFMBE,volume 85))

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Abstract

The decellularized extracellular matrix (ECM) has been established as an ideal biomaterial in tissue engineering applications with potential clinical translation. In the present study, we aimed to generate a hydrogel from decellularized adipose tissue. Adipose tissue samples were collected from the liposuction procedure and subjected to undergo a series of delipidation and decellularization. Oil Red O staining illustrated the complete removal of lipid, whereas the absence of cellular nuclei in histology staining and low DNA residual content indicated an acellular matrix. Decellularized adipose tissue was further fabricated into a hydrogel. Histological evaluation and scanning electron images demonstrated a fibrous ultrastructure of the hydrogel. Furthermore, short-term in vivo observation detected the gelation after 2 h being implanted subcutaneously, which demonstrated dAT hydrogel was capable of self-assembling under physiological condition. Taken together, these results indicated the potential approach of hydrogel from decellularized adipose tissue in tissue engineering.

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Acknowledgements

This research is funded by Vietnam National University Ho Chi Minh City (VNU-HCM) under grant number C2019-18-20.

Conflicts of Interest

The authors have no conflict of interest to declare.

Author information

Authors and Affiliations

  1. Laboratory of Tissue Engineering and Biomedical Materials, University of Science, Ho Chi Minh City, Vietnam

    My Nguyen & Ha Tran

  2. Department of Human Physiology and Animal Biotechnology, Faculty of Biology–Biotechnology, University of Science, Ho Chi Minh City, Vietnam

    My Nguyen & Ha Tran

  3. Vietnam National University, Ho Chi Minh City, Vietnam

    My Nguyen & Ha Tran

  4. Xuan Truong Paradise Plastic Aesthetic, Ho Chi Minh City, Vietnam

    Truong Do

Authors
  1. My Nguyen
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  2. Truong Do
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  3. Ha Tran
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Corresponding author

Correspondence to My Nguyen .

Editor information

Editors and Affiliations

  1. School of Biomedical Engineering, International University—Vietnam National Universities, Ho Chi Minh, Vietnam

    Vo Van Toi

  2. School of Biomedical Engineering, International University—Vietnam National Universities, Ho Chi Minh, Vietnam

    Thi-Hiep Nguyen

  3. School of Biomedical Engineering, International University—Vietnam National Universities, Ho Chi Minh, Vietnam

    Vong Binh Long

  4. School of Biomedical Engineering, International University—Vietnam National Universities, Ho Chi Minh, Vietnam

    Ha Thi Thanh Huong

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Nguyen, M., Do, T., Tran, H. (2022). Fabrication of Injectable Hydrogel from Decellularized Adipose Tissue. In: Van Toi, V., Nguyen, TH., Long, V.B., Huong, H.T.T. (eds) 8th International Conference on the Development of Biomedical Engineering in Vietnam. BME 2020. IFMBE Proceedings, vol 85. Springer, Cham. https://doi.org/10.1007/978-3-030-75506-5_38

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  • DOI: https://doi.org/10.1007/978-3-030-75506-5_38

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  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-030-75505-8

  • Online ISBN: 978-3-030-75506-5

  • eBook Packages: EngineeringEngineering (R0)

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