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Detection of hrHPV DNA with Simulated HPV16 and HPV18 Typing Based on Real-Time PCR

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CMBEBIH 2021 (CMBEBIH 2021)

Part of the book series: IFMBE Proceedings ((IFMBE,volume 84))

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Abstract

The complexity of molecular diagnosis of HPV that is directly related to cervical cancer is caused by a large number of genotypes, such as HPV genotypes 16 and 18, which show the greatest malignant potential and cause 70% of all cervical cancers. The objectives of this study are to determine the significance of Ct values detected by HPV DNA as well as intern control DNA in cervical smear samples and to evaluate the advantages and disadvantages of cervical cancer screening based on HPV DNA and to determine the incidence of HPV16, HPV18 and other mixed infections. In this report, the Abbott RealTime High-Risk HPV Diagnostic Test (Abbott Molecular Inc., IL, USA), for the presence of hrHPV infection, routinely tested a total of 1419 cervical samples of women with cytological changes on the cervix. The average age was 42.59 years. The prevalence of hrHPV infection was 29.81% (hrHPV16, 18 and others). Ct HPV18 showed a significant difference between age groups. Approximately constant values of Ct-IC samples of cervical swabs, Ct values of viral DNA could be considered as a parameter of disease progression/regression. A statistically significant correlation was found between Ct-IC and Ct-HPV16.

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Authors and Affiliations

  1. Faculty of Health Studies, University of Sarajevo, Sarajevo, Bosnia and Herzegovina

    Almedina Hajrović, Emir Šeherčehajić & Adisa Ramić

  2. Medical Biochemical Laboratory, Medlab, Gračanica, Bosnia and Herzegovina

    Arnisa Alibegović

  3. Institute for Clinical Biochemistry and Immunology, Clinical Center University of Sarajevo, Sarajevo, Bosnia and Herzegovina

    Berina Hasanefendić

Authors
  1. Almedina Hajrović
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  2. Arnisa Alibegović
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  3. Emir Šeherčehajić
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  4. Adisa Ramić
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  5. Berina Hasanefendić
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Editors and Affiliations

  1. Verlab Ltd Sarajevo and Faculty of Pharmacy, University of Sarajevo, Sarajevo, Bosnia and Herzegovina

    Almir Badnjevic

  2. Verlab Ltd Sarajevo and International Burch University Sarajevo, Sarajevo, Bosnia and Herzegovina

    Lejla Gurbeta Pokvić

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Hajrović, A., Alibegović, A., Šeherčehajić, E., Ramić, A., Hasanefendić, B. (2021). Detection of hrHPV DNA with Simulated HPV16 and HPV18 Typing Based on Real-Time PCR. In: Badnjevic, A., Gurbeta Pokvić, L. (eds) CMBEBIH 2021. CMBEBIH 2021. IFMBE Proceedings, vol 84. Springer, Cham. https://doi.org/10.1007/978-3-030-73909-6_66

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  • DOI: https://doi.org/10.1007/978-3-030-73909-6_66

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  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-030-73908-9

  • Online ISBN: 978-3-030-73909-6

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