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Monoclonal IgY Antibodies

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IgY-Technology: Production and Application of Egg Yolk Antibodies

Abstract

Monoclonal antibodies (mAbs) have the advantages of high specificity and stable expression. Monoclonal IgY (mIgY) is a new antibody development that combines advantages of IgY and mAbs. mIgY have great potential for immunological detection and diagnosis, for screening and validating biomarkers and drug targets, and for the production of antibodies against conserved mammalian proteins. mIgY can be developed into small molecular weight antibody fragments (particularly scFv), chimeric and humanized antibodies to address the many challenges lying ahead to develop mAb for various biomedical applications, especially those for which conventional antibodies are ineffective. Hybridoma technology or display technology, including phage display, yeast surface display and ribosomal display, can be applied in mIgY generation. However, hybridoma technology is very inefficient in the chicken system due to the lack of a robust avian immortal cell line for hybridoma cell generation. New technologies and different types of antibodies are likely to broaden the application of avian antibodies.

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Change history

  • 16 September 2021

    The original version of the book was inadvertently published with only one of the three affiliations of the volume editor and chapter author Xiao-Ying Zhang.

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Authors and Affiliations

  1. Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, Pulau Pinang, Malaysia

    Chiuan Herng Leow

  2. Shaanxi University of Technology, Hanzhong, China

    Long Xu

  3. University of Minho, Braga, Portugal

    Long Xu

  4. i3S–Instituto de Investigação e Inovação em Saúde and IBMC–Instituto do Biologia Molecular e Celular, University of Porto, Porto, Portugal

    Carol A. Harley

  5. CNC–Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal

    Ricardo S. Vieira-Pires

  6. College of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong, China

    Xiaoying Zhang

  7. Ontario Veterinary College, University of Guelph, Guelph, ON, Canada

    Xiaoying Zhang

  8. CBMA, University of Minho, Braga, Portugal

    Xiaoying Zhang

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  1. Chiuan Herng Leow
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  2. Long Xu
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  3. Carol A. Harley
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  4. Ricardo S. Vieira-Pires
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  5. Xiaoying Zhang
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Correspondence to Chiuan Herng Leow .

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Editors and Affiliations

  1. College of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong, Shaanxi, China

    Xiao-Ying Zhang

  2. Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal

    Ricardo S. Vieira-Pires

  3. School of Natural Sciences, National University of Ireland Galway, Galway, Ireland

    Patricia M. Morgan

  4. Institute of Pharmacology, Charité – Universitätsmedizin Berlin, Berlin, Germany

    Rüdiger Schade

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Leow, C.H., Xu, L., Harley, C.A., Vieira-Pires, R.S., Zhang, X. (2021). Monoclonal IgY Antibodies. In: Zhang, XY., Vieira-Pires, R.S., Morgan, P.M., Schade, R. (eds) IgY-Technology: Production and Application of Egg Yolk Antibodies. Springer, Cham. https://doi.org/10.1007/978-3-030-72688-1_13

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