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Development of a Gelatin-Based Hydrogel to be Used as a Fibrous Scaffold in Myocardial Tissue Engineering

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XXVII Brazilian Congress on Biomedical Engineering (CBEB 2020)

Part of the book series: IFMBE Proceedings ((IFMBE,volume 83))

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Abstract

Myocardial Tissue Engineering (MTE) is a set of techniques that uses biomaterials to reproduce the native extracellular matrix (ECM) of tissues, in this case, the myocardium. The present contribution relates the results of the study about the electrospinning of gelatin, a naturally occurring biopolymer, in view to obtain nanofibrous scaffolds for use in MTE. Gelatin solutions were prepared using gelatin type B and 2,2,2-Trifluoroethanol (TFE) as a solvent. The fibrous were created through electrospinning technique. The voltage, distance and concentration of the solutions were varied to improve the quality of the fibers. The crosslinking method was validated with the use of films, prepared by solvent evaporation technique, with 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC). The morphology of the fibers was investigated by scanning electron microscope (SEM) and the efficiency of the crosslinking processes were evaluated by swelling test and differential scanning calorimetry (DSC). The best fibers were formed (without the presence of drops or beads) by electrospinning a gelatin solution in the following parameters (10% (w/v), 10 kV and 20 cm) or (5% (w/v), 12 kV and 12 cm). The crosslinking process was done using EDC in the films of gelatin. It was possible to observe that different degrees of crosslinking guarantee different properties, such as water swelling as well as different thermal and mechanical properties.

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Acknowledgements

The authors acknowledge recognition of “Central Experimental Multiusuário” from The Universidade Federal do ABC (CEM-UFABC) and the technicians of the biomaterial’s laboratory, by the infra structure and technical support. The financial support received from “Conselho Nacional de Desenvolvimento Científico e Tecnológico—CNPQ—processo 402984/2016-1” and CAPES to the Post-Graduation program of Biomedical Engineering of UFABC.

Conflict of Interest

The authors have declared that there is no conflict of inter-est related to this manuscript.

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Authors and Affiliations

  1. Engenharia Biomédica, Universidade Federal do ABC, Alameda da Universidade, São Bernardo do Campo, Brazil

    C. Parente & S. M. Malmonge

Authors
  1. C. Parente
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  2. S. M. Malmonge
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Editor information

Editors and Affiliations

  1. Department of Electrical Engineering, Universidade Federal do Espírito Santo, Vitória, Brazil

    Teodiano Freire Bastos-Filho

  2. Department of Electrical Engineering, Universidade Federal do Espírito Santo, Vitória, Brazil

    Eliete Maria de Oliveira Caldeira

  3. Department of Electrical Engineering, Universidade Federal do Espírito Santo, Vitória, Brazil

    Anselmo Frizera-Neto

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Parente, C., Malmonge, S.M. (2022). Development of a Gelatin-Based Hydrogel to be Used as a Fibrous Scaffold in Myocardial Tissue Engineering. In: Bastos-Filho, T.F., de Oliveira Caldeira, E.M., Frizera-Neto, A. (eds) XXVII Brazilian Congress on Biomedical Engineering. CBEB 2020. IFMBE Proceedings, vol 83. Springer, Cham. https://doi.org/10.1007/978-3-030-70601-2_25

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  • DOI: https://doi.org/10.1007/978-3-030-70601-2_25

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  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-030-70600-5

  • Online ISBN: 978-3-030-70601-2

  • eBook Packages: EngineeringEngineering (R0)

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