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Biomechanical Analysis of Tissue Engineering Construct for Articular Cartilage Restoration—A Pre-clinical Study

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XXVII Brazilian Congress on Biomedical Engineering (CBEB 2020)

Part of the book series: IFMBE Proceedings ((IFMBE,volume 83))

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Abstract

The chondral lesion and osteoarthritis are conditions associated with an economic burden, since if left untreated may cause changes in the biomechanics of the joint and result in several injuries considered highly disabling to the individual. Mesenchymal Stem Cells (MSCs) have the immunomodulatory capacity and paracrine signaling that are useful for tissue bioengineering to treat bone and cartilage injuries. To the best of our knowledge, there is no institution in Brazil studying cartilage biomechanical properties in Good Manufacturing Practice (GMP) technique. Therefore, this study aims to describe biomechanics analysis for cartilage restoration by tissue engineering and cell therapy treatments in a GMP translational large animal model. A controlled experimental study in fourteen Brazilian miniature pigs was performed, using scaffold-free Tissue Engineering Construct (TEC) from dental pulp and synovial MSCs with 6 months follow-up. To compare the cartilage with and without TEC, indentation and maximum compressive tests were performed, as well as Finite Element model to simulate the osteochondral block and characterize its properties. The Young’s Modulus of each sample was determined, and the outcomes of maximum compressive test demonstrated the cartilage integrity. The proposed method was feasible and capable to properly evaluate articular cartilage restoration.

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Acknowledgements

This study was supported by Fundação de Amparo a Pesquisa do Estado de São Paulo (FAPESP–Process 2017/05774-5), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES–Process 88881.171651/2018-01), grant from the International Society of Arthroscopy, Knee Surgery and Orthopaedic Sports Medicine and the Orthopaedic Research and Education Foundation (ISAKOS Osteoarthritis Grant—2018). This study was performed at Hospital Sírio Libanês, São Paulo, SP, Brazil; Hospital das Clínicas HCFMUSP, Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP, Brazil; and Instituto do Coração INCOR, São Paulo, SP, Brazil.

Conflict of Interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Institute of Orthopedics and Traumatology, Hospital das Clínicas, Faculdade de Medicina da Universidade de São Paulo, 333 Dr. Ovídio Pires de Campos, 05403-010, São Paulo, SP, Brazil

    R. R. de Faria, A. J. Hernandez & T. L. Fernandes

  2. Hospital Sírio Libanês, São Paulo, SP, Brazil

    D. F. Bueno & T. L. Fernandes

  3. Pontifícia Universidade Católica de São Paulo (PUC-SP), São Paulo, SP, Brazil

    R. R. de Faria

  4. Instituto do Coração (InCor)/Bioengenharia, Hospital das Clínicas da FMUSP, São Paulo, Brazil

    M. J. S. Maizato & I. A. Cestari

  5. Centro Universitário FEI, São Paulo, Brazil

    R. Bortolussi & C. Albuquerque

Authors
  1. R. R. de Faria
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  2. M. J. S. Maizato
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  3. I. A. Cestari
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  4. A. J. Hernandez
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  5. D. F. Bueno
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  6. R. Bortolussi
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  7. C. Albuquerque
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  8. T. L. Fernandes
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Corresponding author

Correspondence to T. L. Fernandes .

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Editors and Affiliations

  1. Department of Electrical Engineering, Universidade Federal do Espírito Santo, Vitória, Brazil

    Teodiano Freire Bastos-Filho

  2. Department of Electrical Engineering, Universidade Federal do Espírito Santo, Vitória, Brazil

    Eliete Maria de Oliveira Caldeira

  3. Department of Electrical Engineering, Universidade Federal do Espírito Santo, Vitória, Brazil

    Anselmo Frizera-Neto

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de Faria, R.R. et al. (2022). Biomechanical Analysis of Tissue Engineering Construct for Articular Cartilage Restoration—A Pre-clinical Study. In: Bastos-Filho, T.F., de Oliveira Caldeira, E.M., Frizera-Neto, A. (eds) XXVII Brazilian Congress on Biomedical Engineering. CBEB 2020. IFMBE Proceedings, vol 83. Springer, Cham. https://doi.org/10.1007/978-3-030-70601-2_22

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  • DOI: https://doi.org/10.1007/978-3-030-70601-2_22

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  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-030-70600-5

  • Online ISBN: 978-3-030-70601-2

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