Abstract
The present work describes the technological development of a low-cost and miniaturized instrument to Polymerase Chain Reaction (PCR) for detection of Sars-CoV-2 ribonucleic acid (RNA) and, potentially, an open platform for detection of other microorganisms. Most devices use a big aluminum bar coupled to a peltier to heat and cool the reaction tube; however, a lot of energy is wasted in the process. To take advantage of the energy and reduce cost–benefit of the device, we introduced the Joule Effect in the printed circuit board for heating samples, and a computer fan for cooling. Other improvements such as a precise heating sample spot, and a LM35 thermal sensor with a PID (proportional integral-derivative) algorithm to control the circuit temperature, have also been included. The processes were carried out based on cost–benefit and performance to bring to the market a robust detection platform for in vitro diagnostic tests.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Mullis KB, Faloona FA (1987) Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction. Methods Enzymol. 155(1987):335–350. https://doi.org/10.1016/0076-6879(87)55023-6
Mullis K, Faloona F, Scharf S, Saiki R, Horn G, Erlich H (1986) Specific enzymatic amplification of DNA in vitro: the polymerase chain reaction. Cold Spring Harb Symp Quant Biol. 51(1986):263–273. https://doi.org/10.1101/sqb.1986.051.01.032
Erlich HA (2015) PCR technology: principles and applications for DNA amplification. https://doi.org/10.1007/978-1- 349-20235-5
Innis MA, Gelfand DH, Sninsky JJ, White TJ (2012) PCR protocols: a guide to methods and applications, Academic press
Njiru, ZK (2012) “Loop-mediated isothermal amplification technology: towards point of care diagnostics.” PLoS neglected tropical diseases 6.6 : e1572
Instruction manual of Molecular IVD commercial product at www.ibmp.org.br/pt-br/info/
Summary of Available In-House Assays Protocols at https://www.who.int/docs/default- source/coronaviruse/whoinhouseassays.pdf
Acknowledgements
This work was supported by the Oswaldo Cruz Foundation (Fiocruz), Universidade Tecnológica Federal do Paraná (UTFPR), Instituto Carlos Chagas (ICC-Fiocruz PR), Instituto de Biologia Molecular do Paraná (IBMP), The Brazilian National Council for Scientific and Technological Development (CNPq), Fundação para o Desenvolvimento Científico e Tecnológico em Saúde (Fiotec), and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior-Brasil (CAPES)-Código de Financiamento 001.
Conflict of Interest
The authors certify that there is no actual or potential conflict of interest in relation to this article.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2022 Springer Nature Switzerland AG
About this paper
Cite this paper
Nascimento, L.R. et al. (2022). Technological Development of a Multipurpose Molecular Point-of-Care Device for Sars-Cov-2 Detection. In: Bastos-Filho, T.F., de Oliveira Caldeira, E.M., Frizera-Neto, A. (eds) XXVII Brazilian Congress on Biomedical Engineering. CBEB 2020. IFMBE Proceedings, vol 83. Springer, Cham. https://doi.org/10.1007/978-3-030-70601-2_177
Download citation
DOI: https://doi.org/10.1007/978-3-030-70601-2_177
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-030-70600-5
Online ISBN: 978-3-030-70601-2
eBook Packages: EngineeringEngineering (R0)