Abstract
Cleaning and disinfecting surfaces and materials in health services are primary elements in infection control measures. For thermosensitive materials, the chemical agents used have disadvantages such as the odor of the products, which can cause allergic reactions to patients and the nursing staff. Photodynamic therapy (PDT) has been shown to be an effective technique in the treatment of infections caused by different microorganisms; however, nothing is known about the effects of this technique on the microstructure of hospital supplies. This in vitro study aimed to evaluate the effects of 0.2% peracetic acid, 1% sodium hypochlorite and PDT with 0.01% methylene blue on the composition and color changes of hospital masks and extensions. For this purpose, 100 mask samples and 100 extension samples were randomly distributed in 20 experimental groups (n = 10, 10 groups for each material), in which the applied substance was varied (sodium hypochlorite, peracetic acid and PDT) and the number of applications (without application, 1, 2 or 3 applications). The compositional analysis was performed by Fourier transform infrared spectroscopy, while the color changes were evaluated using image analysis by CIElab method evaluating the parameters L*, a*, b* and ΔE. The statistical analysis was performed at 5% significance level. It was observed that all agents altered the composition of the materials in a similar way. Although all agents promoted changes in different parameters evaluated, peracetic acid and methylene blue alone altered the final color perceived only in extensions. It was concluded that 0.2% peracetic acid, 1% sodium hypochlorite and PDT alter the chemical composition of both masks and extensions, and that such changes have a positive relationship with the number of treatments performed. These compositional changes may be related to the color changes promoted in both materials by all agents tested.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
BRASIL (2010) Agência Nacional de Vigilância Sanitária. Segurança do paciente em serviços de saúde: limpeza e desinfecção de superfícies. Agência Nacional de Vigilância Sanitária, Brasília
Centers for Diseases Control and Prevention (2008) Guideline for disinfection and sterization in healthcare facilities. Atlanta, EUA
Poletto AP, Hoss J et al (2016) Efeito do ácido peracético sobre a adesão microbiana e as propriedades de tubos endotraqueais. Rev Fac Odontol Passo Fundo 21:196–200
Estrela C, Estrela CRA et al (2002) Mechanism of action of sodium hypochlorite. Braz Dent J 13:113–117
Tardivo JP, Giglio AD, Oliveira CS et al (2005) Methylene blue in photodynamic therapy: from basic mechanisms to clinical applications. Photodiagnosis Photodyn Ther 2:175–191
Cal E, Guneri P, Kose T Comparison of digital and spectrophotometric measurements of colour shade guides. J Oral Rehab 33:221–228
Bernard L, Dévaudin B, Lecoeur M et al (2014) Analytical methods for the determination of DEHP plasticizer alternatives present in medical devices: a review. Talanta 129:39–54
Acknowledgements
To FAPESP (2017-21887-4), PROCAD-CAPES (88881.068505/2014-01), National Institute of Photonics (CNPq/INCT 465763/2014-6) and Multiuser Experimental Center of UFABC (CEM-UFABC). The authors also thank Prof. Emery C.C.C. Lins (UFPE) for access to the imaging equipment used in this study.
Conflict of Interest
The authors declare that they have no conflict of interest.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2022 Springer Nature Switzerland AG
About this paper
Cite this paper
Namba, A.F., Del-Valle, M., Daghastanli, N.A., Ana, P.A. (2022). In Vitro Study of the Microstructural Effects of Photodynamic Therapy in Medical Supplies When Used for Disinfection. In: Bastos-Filho, T.F., de Oliveira Caldeira, E.M., Frizera-Neto, A. (eds) XXVII Brazilian Congress on Biomedical Engineering. CBEB 2020. IFMBE Proceedings, vol 83. Springer, Cham. https://doi.org/10.1007/978-3-030-70601-2_176
Download citation
DOI: https://doi.org/10.1007/978-3-030-70601-2_176
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-030-70600-5
Online ISBN: 978-3-030-70601-2
eBook Packages: EngineeringEngineering (R0)