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Development and Experimentation of a rTMS Device for Rats

  • Conference paper
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XXVII Brazilian Congress on Biomedical Engineering (CBEB 2020)

Part of the book series: IFMBE Proceedings ((IFMBE,volume 83))

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Abstract

Neuropathic pain (NP) is related to the presence of hyperalgesia, allodynia and spontaneous pain, affecting 7–10% of the general population. Repetitive transcranial magnetic stimulation (rTMS) is being applied for NP relief especially in patients with refractory pain.

Objective: As NP response to existing treatments is often insufficient, we aimed to develop a magnetic stimulator and customized coils and evaluate rTMS treatment over the nociceptive response of rats submitted to a NP model.

Methods: The magnetic stimulator and the butterfly coils were developed in the Biomedical Engineering lab of Hospital de Clínicas de Porto Alegre. The device generated pulses with a 1 ms pulse width in a 1 Hz frequency. A total of 106 adult (60-days old) male Wistar rats were divided into 9 experimental groups: control (C), control plus sham rTMS (C + s.rTMS), control plus rTMS (C + rTMS), sham neuropathic pain (s.NP), sham neuropathic pain plus sham rTMS (s.NP + s.rTMS), sham neuropathic pain plus rTMS (s.NP + rTMS), neuropathic pain (NP), neuropathic pain + sham rTMS (NP + s.rTMS) and neuropathic pain plus rTMS (NP + rTMS). NP establishment was achieved 14 days after the surgery for chronic constriction injury (CCI) of the sciatic nerve, and rats were treated with daily 5-min sessions of rTMS for 8 consecutive days. Nociceptive behavior was assessed by von Frey and Hot Plate tests at baseline, after NP establishment and post-treatment.

Results: The measurement of magnetic field intensity 2.5 mm and 5.0 mm from the coil center on the 90º axis showed 160 mT and 125 mT respectively. rTMS treatment promoted a partial reversal of the mechanical allodynia and a total reversal of the thermal hyperalgesia induced by CCI.

Conclusions: We presume that low-frequency rTMS is a potential tool for NP treatment, possibly due to the modulation of plasticity and promotion of an analgesic effect.

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Acknowledgements

This research was supported by the following Brazilian funding agencies: CNPq, CAPES, GPPG/HCPA (FIPE Grant 2017-0438); FAPERGS (Grant PRONEM 16/2551-0000249-5).

Conflict of Interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Serviço de Pesquisa e Desenvolvimento em Engenharia Biomédica, Hospital de Clínicas de Porto Alegre, Rua Ramiro Barcelos, 2350, Porto Alegre, Brazil

    P. R. S. Sanches, D. P. Silva, A. F. Müller, P. R. O. Thomé, A. C. Rossi & B. R. Tondin

  2. Laboratório de Farmacologia da Dor e Neuromodulacão: Ensaios Pré-Clínicos, Centro de Pesquisa Experimental, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil

    R. Ströher, L. Santos & I. L. S. Torres

  3. Programa de Pós-Graduação em Ciências Biológicas: Farmacologia e Terapêutica - Instituto de Ciências Básicas da Saúde - Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil

    R. Ströher, L. Santos & I. L. S. Torres

Authors
  1. P. R. S. Sanches
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  2. D. P. Silva
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  3. A. F. Müller
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  4. P. R. O. Thomé
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  5. A. C. Rossi
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  6. B. R. Tondin
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  7. R. Ströher
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  9. I. L. S. Torres
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Correspondence to P. R. S. Sanches .

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Editors and Affiliations

  1. Department of Electrical Engineering, Universidade Federal do Espírito Santo, Vitória, Brazil

    Teodiano Freire Bastos-Filho

  2. Department of Electrical Engineering, Universidade Federal do Espírito Santo, Vitória, Brazil

    Eliete Maria de Oliveira Caldeira

  3. Department of Electrical Engineering, Universidade Federal do Espírito Santo, Vitória, Brazil

    Anselmo Frizera-Neto

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Sanches, P.R.S. et al. (2022). Development and Experimentation of a rTMS Device for Rats. In: Bastos-Filho, T.F., de Oliveira Caldeira, E.M., Frizera-Neto, A. (eds) XXVII Brazilian Congress on Biomedical Engineering. CBEB 2020. IFMBE Proceedings, vol 83. Springer, Cham. https://doi.org/10.1007/978-3-030-70601-2_119

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  • DOI: https://doi.org/10.1007/978-3-030-70601-2_119

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  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-030-70600-5

  • Online ISBN: 978-3-030-70601-2

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