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MALDI-TOF-Based Identification of Dermatophytes

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Dermatophytes and Dermatophytoses

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Abstract

Dermatophytes and other keratinophilic fungi are common pathogens. Yet, their correct morphological identification remains a challenge for the mycologist. In the past few years, matrix-assisted laser desorption/ionization–time-of-flight mass spectrometry (MALDI-TOF MS) has become a widely used technique for pathogen identification in microbiology laboratories. The application of mass spectrometry for the identification of dermatophytes faces specific difficulties and benefits from a limited number of studies. In this chapter, we review the general principles of mass spectrometry and detail the technical aspects, such as sample preparation, type of targets, and scope of the different matrixes available. Various systems are currently available that use different basic spectrum processing and variable identification algorithms. We describe these different systems and the different databases available to date and their respective performance for the identification of dermatophytes and propose a review of the literature.

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Authors and Affiliations

  1. AP-HP, Groupe Hospitalier La Pitié-Salpêtrière, Service de Parasitologie Mycologie, Paris, France

    Anne-Cécile Normand, Arnaud Fekkar & Renaud Piarroux

  2. AP-HP, Groupe Hospitalier Saint-Antoine, Service de Parasitologie Mycologie, Paris, France

    Alicia Moreno-Sabater

  3. Sorbonne Université, Inserm, CNRS, Centre d’Immunologie et des Maladies Infectieuses, Cimi-Paris, Paris, France

    Alicia Moreno-Sabater & Arnaud Fekkar

  4. Sorbonne Université, Inserm, Institut Pierre Louis d’Epidemiologie et de Santé Publique, Paris, France

    Renaud Piarroux

Authors
  1. Anne-Cécile Normand
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  2. Alicia Moreno-Sabater
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  3. Arnaud Fekkar
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  4. Renaud Piarroux
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Correspondence to Renaud Piarroux .

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Editors and Affiliations

  1. Univ Angers, Université de Bretagne Occidentale, CHU, SFR ICAT, Groupe d’Etude des Interactions Hôte-Pathogène (GEIHP, EA 3142), Angers, France

    Jean-Philippe Bouchara

  2. Laboratory for Medical Microbiology, Rötha, Sachsen, Germany

    Pietro Nenoff

  3. Mediprobe Research Inc., London, ON, Canada

    Aditya K. Gupta

  4. New York State Department of Health Wadsworth Center, Mycology Laboratory, Albany, NY, USA

    Vishnu Chaturvedi

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Normand, AC., Moreno-Sabater, A., Fekkar, A., Piarroux, R. (2021). MALDI-TOF-Based Identification of Dermatophytes. In: Bouchara, JP., Nenoff, P., Gupta, A.K., Chaturvedi, V. (eds) Dermatophytes and Dermatophytoses. Springer, Cham. https://doi.org/10.1007/978-3-030-67421-2_18

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