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Non-Destructive and Label-Free Monitoring of 3D Cell Constructs

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Basic Concepts on 3D Cell Culture

Part of the book series: Learning Materials in Biosciences ((LMB))

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What You Will Learn in This Chapter

Monitoring cells in 3D culture is a big challenge in cell biology and biomedical research. In this chapter, we will demonstrate the great potential of Raman trapping microscopy coupled with multivariate statistical analysis, to monitor individual cells in 2D cultures and 3D tissues. We shortly describe the concept of spontaneous Raman spectroscopy using a bio-compatible 785 nm laser and the advantages of combined Optical Trapping. Furthermore, you will learn how to extract the rich chemical information from the Raman spectra using a unique Raman data analyzing software dedicated to biomedical needs. We show that Raman allows to monitor composition, functionality and quality of keratinocytes and fibroblasts during production as well as within the final graft. Furthermore, Raman allows to observe cell vitality within microspheres of mouse embryonic stem cells giving the potential to monitor drug penetration.

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Further Reading

  • Chapter (Raman Trapping Microscopy for Non-invasive Analysis of Biological Samples). Animal cell biotechnology: methods and protocols, methods in molecular biology. Cham: Springer; 2020.

    Google Scholar 

  • Steinke M, Gross R, Walles H, et al. An engineered 3D human airway mucosa model based on an SIS scaffold. Biomaterials. 2014;35:7355–62.

    Article  CAS  Google Scholar 

  • Charwat V, Schütze K, Holnthoner W, et al. Potential and limitations of microscopy and Raman spectroscopy for live-cell analysis of 3D cell cultures. J Biotechnol. 2015;205:70–81.

    Article  CAS  Google Scholar 

  • Pudlas M, Koch S, Bolwien C, Walles H. Raman spectroscopy as a tool for quality and sterility analysis for tissue engineering applications like cartilage transplants. Int J Artif Organs. 2010;33:228–37.

    Article  CAS  Google Scholar 

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Acknowledgement

We would like to thank Prof. Dr. Ernst Reichmann, TBRU (University-Children’s Hospital Zürich, Switzerland) as well as Dr. Daniela Marino CUTISS AG (Zürich, Switzerland) for providing the skin cell samples. We would like also to thank Sarvesh Ghorpade, Dr. Heidi Kremling and Florian Zunhammer for their assistance. We thank also Dr. Ali Mirsaidi and Dr. Patrick Kugelmeier from Kugelmeiers Ltd. (Erlenbach, Switzerland) for providing the microspheres and for their great support.

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Authors and Affiliations

  1. CellTool GmbH, Lindemannstraße, Tutzing, Germany

    Hesham K. Yosef & Karin Schütze

Authors
  1. Hesham K. Yosef
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  2. Karin Schütze
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Corresponding author

Correspondence to Karin Schütze .

Editor information

Editors and Affiliations

  1. Institute of Cell and Tissue Culture Technologies, University of Natural Resources and Life Sciences, Vienna, Austria

    Cornelia Kasper

  2. Institute of Cell and Tissue Culture Technologies, University of Natural Resources and Life Sciences, Vienna, Austria

    Dominik Egger

  3. Institute of Technical Chemistry, Leibniz University of Hannover, Hannover, Germany

    Antonina Lavrentieva

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Yosef, H.K., Schütze, K. (2021). Non-Destructive and Label-Free Monitoring of 3D Cell Constructs. In: Kasper, C., Egger, D., Lavrentieva, A. (eds) Basic Concepts on 3D Cell Culture . Learning Materials in Biosciences. Springer, Cham. https://doi.org/10.1007/978-3-030-66749-8_10

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