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Non-invasive Sampling Techniques Applied to Conservation Genetic Studies in Mammals

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Molecular Ecology and Conservation Genetics of Neotropical Mammals

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Abstract

Non-invasive samples have been used in Conservation Genetics and Molecular Ecology studies since the 1990s. The continuous advances in DNA extraction, amplification, and data analysis techniques have made them the perfect choice for the study of endangered populations and species. In the first part of this chapter, we carry out a historical review of sampling techniques used for genetic studies. Then, we analyze what type of samples, molecular markers, and sampling techniques are the most used for the genetic study of Neotropical mammals from samples obtained by non-invasive methods. Later, we review what are the advantages and limitations of the use of this type of samples as well as the possible solutions to the main problems associated with their use, and finally, we assess the future challenges of using non-invasive sampling. Our review also includes several case studies in which different sources of DNA and several molecular markers are used to address genetic studies in Neotropical mammals.

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Authors and Affiliations

  1. Departamento de Ciencias Básicas, Universidad Nacional de Luján, Luján, Argentina

    M. Soledad Byrne, Ezequiel A. Ibañez, Maximiliano Nardelli & Juan Ignacio Túnez

  2. Grupo de Investigación en Ecología Molecular, Instituto de Ecología y Desarrollo Sustentable (INEDES-UNLU-CONICET-CIC), Luján, Argentina

    M. Soledad Byrne, Diego M. Peralta, Ezequiel A. Ibañez, Maximiliano Nardelli & Juan Ignacio Túnez

  3. Departamento de Educación, Universidad Nacional de Luján, Luján, Argentina

    Maximiliano Nardelli

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  1. M. Soledad Byrne
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  2. Diego M. Peralta
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  3. Ezequiel A. Ibañez
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  4. Maximiliano Nardelli
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  1. Departamento de Educación, National University of Luján, Luján, Argentina

    Maximiliano Nardelli

  2. Grupo de Investigación en Ecología Molecular, Instituto de Ecología y Desarrollo Sustentable, Luján, Argentina

    Juan Ignacio Túnez

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Byrne, M.S., Peralta, D.M., Ibañez, E.A., Nardelli, M., Túnez, J.I. (2021). Non-invasive Sampling Techniques Applied to Conservation Genetic Studies in Mammals. In: Nardelli, M., Túnez, J.I. (eds) Molecular Ecology and Conservation Genetics of Neotropical Mammals. Springer, Cham. https://doi.org/10.1007/978-3-030-65606-5_4

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