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Artificial Small RNAs for Functional Genomics in Plants

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RNA-Based Technologies for Functional Genomics in Plants

Abstract

RNA interference (RNAi) is based on the sequence-specific degradation of target RNAs by highly complementary small RNAs (sRNAs), which can be engineered to selectively target genes of interest. In plants, artificial microRNAs (amiRNAs) and artificial/synthetic trans-acting small interfering RNAs (atasi/syn-tasiRNAs) are the two main classes of artificial small RNAs (art-sRNAs). Art-sRNAs are refined, highly specific, selective, and potent RNAi tool that has been extensively used in gene function studies and for crop improvement. Here we describe the biogenesis and function of art-sRNAs, and how they are designed and used to study the function of plant genes.

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Acknowledgements

This work was supported by grants from Ministerio de Ciencia, Innovación y Universidades (MCIU, Spain), Agencia Estatal de Investigación (AEI, Spain), and Fondo Europeo de Desarrollo Regional (FEDER, European Union) [RTI2018-095118-A-100 and RYC-2017-21648 to A.C., and PRE2019-088439 to A.E.C.], and from Consejo Superior de Investigaciones Científicas (CSIC, Spain) [JAEINT_20_01312 to T.M.G].

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Correspondence to Alberto Carbonell .

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Cisneros, A.E., de la Torre-Montaña, A., Martín-García, T., Carbonell, A. (2021). Artificial Small RNAs for Functional Genomics in Plants. In: Tang, G., Teotia, S., Tang, X., Singh, D. (eds) RNA-Based Technologies for Functional Genomics in Plants. Concepts and Strategies in Plant Sciences. Springer, Cham. https://doi.org/10.1007/978-3-030-64994-4_1

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