Abstract
The present study was taken up to test the soil sample isolated from an automobile workshop located at Coimbatore for the presence of biosurfactant producing bacteria. Ten isolates were initially screened from the soil collected from the petrochemical exposed site. Isolation was done in the minimal salt medium, and this was followed by screening for the biosurfactant production through three different methods, namely emulsification index, oil displacement activity and hemolytic assay. In comparison with all the three screening methods, two best isolates were chosen and was then subjected to mass production in the mineral salt medium for biosurfactants generation. The isolate identified as Serratia marcescens through 16S rRNA sequencing. Pinkish red-coloured occurrence was observed during the mass production, which may be possibly due to pigmentation. The extracted biosurfactants were analyzed with Fourier transform infrared spectroscopy spectra. The potent nature of the soil collected from the contaminated petrochemical site for the production of biosurfactants has a remarkable growth prospective, sourcing as a better replacement to the increasing environmental concern associated with the chemical surfactants.
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Vijayalekshmi, V., Muthukumaran, P., Jeyaseelan, A. (2021). Screening, Isolation and Molecular Characterization of Biosurfactants Producing Serratia Marcescens from Petrochemical Exposed Site. In: Marimuthu, P.D., Sundaram, R., Jeyaseelan, A., Kaliannan, T. (eds) Bioremediation and Green Technologies. Environmental Science and Engineering(). Springer, Cham. https://doi.org/10.1007/978-3-030-64122-1_17
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