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RNA Interference (RNAi) in Functional Genomics of Wheat

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Genome Engineering for Crop Improvement

Part of the book series: Concepts and Strategies in Plant Sciences ((CSPS))

Abstract

Post-genomic era of biology is faced with a major challenge in deciphering the gene function out of enormous amount of data generated by NGS technology, in addition to large number of EST sequences, available in the public domain. Among many approaches, one important approach is to knock out the gene and analysis of the visible effect of loss of gene function. RNA interference (RNAi), as a reverse genetic approach currently in use for studies of gene function, holds a great promise in this context. The efficacy of RNAi as tool for functional genomics study has already been successfully demonstrated in Caenorhabditis elegans. With the availability of comprehensive resources of genomic sequence data and knowledge of the biological mechanism of RNAi, use of RNAi in functional genomics is quickly gaining space and popularity. Results from the study of transgene-induced RNAi suggests many variables that should be considered while designing experiment to decipher gene function.

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Authors and Affiliations

  1. Department of Agricultural Biotechnology, Assam Agricultural University, Jorhat, 785013, India

    Priyabrata Sen

  2. National Institute of Science Communication and Information Resources, New Delhi, 110012, India

    Charu Lata

  3. ICAR-National Institute for Plant Biotechnology, LBS building, Pusa Campus, New Delhi, 110012, India

    Kanti Kiran & Tapan Kumar Mondal

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  1. Priyabrata Sen
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  2. Charu Lata
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  3. Kanti Kiran
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  4. Tapan Kumar Mondal
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Correspondence to Priyabrata Sen .

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Editors and Affiliations

  1. ICAR-National Professor Programme (Norman Borlaug Chair) and DBT-North East Centre for Agricultural Biotechnology, Assam Agricultural University, Jorhat, Assam, India

    Bidyut Kumar Sarmah

  2. Department of Agricultural Biotechnology and ICAR-National Professor Programme (Norman Borlaug Chair), Assam Agricultural University, Jorhat, Assam, India

    Basanta Kumar Borah

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Sen, P., Lata, C., Kiran, K., Mondal, T.K. (2021). RNA Interference (RNAi) in Functional Genomics of Wheat. In: Sarmah, B.K., Borah, B.K. (eds) Genome Engineering for Crop Improvement. Concepts and Strategies in Plant Sciences. Springer, Cham. https://doi.org/10.1007/978-3-030-63372-1_10

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