Abstract
We present a new method for controllable loading of cell models in an ultrasonic (20 kHz) regime. The protocol is based on the inertial-based ultrasonic shaking test and allows to deform cells in the range of few mm/m to help understand potential consequences of repeated loading characteristic of ultrasonic cutting.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Amin, A.K., Huntley, J.S., Bush, P.G., Simpson, A.H., Hall, A.C.: Chondrocyte death in mechanically injured articular cartilage: the influence of extracellular calcium. J. Orthop. Res. 27(6), 778–784 (2009)
Marrelli, M., et al.: Dental pulp stem cell mechanoresponsiveness: effects of mechanical stimuli on dental pulp stem cell behavior. Front. Physiol. 9, 1685 (2018)
Uddin, S.M., et al.: Chondro-protective effects of low intensity pulsed ultrasound. Osteoarthr. Cartil. 24(11), 1989–1998 (2016)
Manaka, S., et al.: Low-intensity pulsed ultrasound-induced ATP increases bone formation via the P2X7 receptor in osteoblast-like MC3T3-E1 cells. FEBS Lett. 589(3), 310–318 (2015)
Kudo, N.: High-speed in situ observation system for sonoporation of cells with size- and position-controlled microbubbles. IEEE Trans. Ultrason. Ferroelectr. Freq. Control. 64(1), 273–280 (2017)
Yuan, F., Yang, C., Zhong, P.: Cell membrane deformation and bioeffects produced by tandem bubble-induced jetting flow. Proc. Natl. Acad. Sci. U. S. A. 112(51), E7039–E7047 (2015)
Gao, Q., Cooper, P.R., Walmsley, A.D., Scheven, B.A.: Role of Piezo channels in ultrasound-stimulated dental stem cells. J. Endod. 43(7), 1130–1136 (2017)
Gao, Q., Walmsley, A.D., Cooper, P.R., Scheven, B.A.: Ultrasound stimulation of different dental stem cell populations: role of mitogen-activated protein kinase signaling. J. Endod. 42(3), 425–431 (2016)
Seghir, R., Pierron, F.: A novel image-based ultrasonic test to map material mechanical properties at high strain-rates. Exp. Mech. 58(2), 183–206 (2017)
Grédiac, M., Sur, F., Blaysat, B.: The grid method for in-plane displacement and strain measurement: a review and analysis. Strain. 52(3), 205–243 (2016)
Pereno, V., et al.: Electroformation of giant unilamellar vesicles on stainless steel electrodes. ACS Omega. 2(3), 994–1002 (2017)
Trujillo-Pino, A., Krissian, K., Alemán-Flores, M., Santana-Cedrés, D.: Accurate subpixel edge location based on partial area effect. Image Vis. Comput. 31(1), 72–90 (2013)
Acknowledgements
The authors gratefully acknowledge EPSRC for funding through grants EP/L026910/1 and EP/R045291/1.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2021 The Society for Experimental Mechanics, Inc.
About this paper
Cite this paper
Marek, A., De Grazia, A., Régal, X., Carugo, D., Pierron, F. (2021). Quantifying Ultrasonic Deformation of Cell Membranes with Ultra-High-Speed Imaging. In: Notbohm, J., Karanjgaokar, N., Franck, C., DelRio, F.W. (eds) Mechanics of Biological Systems and Materials & Micro-and Nanomechanics & Research Applications. Conference Proceedings of the Society for Experimental Mechanics Series. Springer, Cham. https://doi.org/10.1007/978-3-030-59765-8_5
Download citation
DOI: https://doi.org/10.1007/978-3-030-59765-8_5
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-030-59764-1
Online ISBN: 978-3-030-59765-8
eBook Packages: EngineeringEngineering (R0)