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Biomechanical Testing of Human Red Blood Cells Under Controlled Oxygen Tension

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Mechanics of Biological Systems and Materials & Micro-and Nanomechanics & Research Applications

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Abstract

Red blood cells are subjected to dynamic loads of shear and oxidative stresses when they traverse through the blood circulation system. Cell membranes are not only subjected to mechanical forces but also oxidative damage as a result of hypoxia. We develop an experimental strategy that can subject biological cells to well-controlled shear and gaseous microenvironment in a microfluidic device. Significant changes in cell deformability and relaxation characteristics due to variation in oxygen tension are observed and quantified. Our results demonstrate that hypoxia can lead to significant loss of deformability in red blood cells. The relationship between the membrane fatigue degradation and the repeated stressing conditions of blood circulation can be further investigated using the developed platform.

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Acknowledgements

This work was supported by the NSF Grant No. 1635312, No. 1464102, and NIH Grant 1OT2HL152638. E.D. and Y.Q. acknowledge support from NIH Grant 5R01EB025819.

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Authors and Affiliations

  1. Department of Ocean and Mechanical Engineering, College of Engineering and Computer Science, Florida Atlantic University, Boca Raton, FL, USA

    E. Du & Yuhao Qiang

Authors
  1. E. Du
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  2. Yuhao Qiang
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Corresponding author

Correspondence to E. Du .

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Editors and Affiliations

  1. 533 Engineering Research Building, University of Wisconsin-Madison, Madison, WI, USA

    Jacob Notbohm

  2. 242 Higgins Lab, Worchester Polytech Institute, Worcester, MA, USA

    Nikhil Karanjgaokar

  3. Brown University, Providence, RI, USA

    Christian Franck

  4. Sandia National Laboratories, Albuquerque, MN, USA

    Frank W. DelRio

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Du, E., Qiang, Y. (2021). Biomechanical Testing of Human Red Blood Cells Under Controlled Oxygen Tension. In: Notbohm, J., Karanjgaokar, N., Franck, C., DelRio, F.W. (eds) Mechanics of Biological Systems and Materials & Micro-and Nanomechanics & Research Applications. Conference Proceedings of the Society for Experimental Mechanics Series. Springer, Cham. https://doi.org/10.1007/978-3-030-59765-8_11

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  • DOI: https://doi.org/10.1007/978-3-030-59765-8_11

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  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-030-59764-1

  • Online ISBN: 978-3-030-59765-8

  • eBook Packages: EngineeringEngineering (R0)

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