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Selected Reaction Monitoring Mass Spectrometry

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Selected Reaction Monitoring Mass Spectrometry (SRM-MS) in Proteomics

Abstract

Proteomics is the large-scale study of the entire complement of proteins in a cell, tissue, biofluid, or organism. Mass spectrometry-based proteomics can be done by top-down, middle-down, or bottom-up approach. Proteomics workflow is also divided into shotgun/discovery and targeted proteomics. SRM-MS or MRM-MS is one of most prominent targeted proteomics techniques initially developed for small molecules but now used largely for proteomics. The method utilizes triple quadrupole (QqQ) instrument, where Q1 and Q3 are set for a specified precursor m/z and its specific fragment ion after it fragments in the q2 collision cell. Several such transitions (precursor > product ion pairs) can be monitored over the chromatographic elusion time. Researchers nowadays also use SRM assays with some modifications of the conventional one. Other targeted proteomics methods include pseudoSRM, PRM, and targeted DIA.

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  1. Sanofi Genzyme, Framingham, MA, USA

    Mahmud Hossain

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Hossain, M. (2020). Selected Reaction Monitoring Mass Spectrometry. In: Selected Reaction Monitoring Mass Spectrometry (SRM-MS) in Proteomics. Springer, Cham. https://doi.org/10.1007/978-3-030-53433-2_3

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