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Shockwave-Generating Interdisciplinary Methods Used to Elaborate Acellular Tissue Origin Extracellular Matrix

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Innovations in Biomedical Engineering (AAB 2020)

Abstract

In the work, as the substrate functional scaffold for the cell culture was prepared from the animal aortic valve. The protocol for acquisition fragments of tissue has been developed. Properly crafted tissue was subjected by the acellularization process, which means the removal of cells from the tissue in order to obtain pure, non-degraded extracellular matrix. Elimination of cells from the tissue was carried out by unconventional methods of materials science involving a laser ablation with different power and beam configurations. Physical processes were designed to cause the elimination of tissue cells. The issue of the cell elimination from tissue, and then the formation of new tissue on the basis of the extracellular matrix (ECM) meets strong clinical interest, but at the moment states a complex issue from a scientific point of view.

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Acknowledgements

The main theme of the work concerns the statutory works of the Institute of Metallurgy and Materials Science PAS “Z-2” and the research was financially supported by the Project no. 2016/23/B/ST8/01481 “Interdisciplinary methods of creating and functioning of biomimetic materials based on Animal origin extracellular matrix” of the Polish National Center of Science. The research was co-financed by the European Union from the resources of the European Social Fund (Project No.WND-POWR.03.02.00-00-I043/16).

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Correspondence to Roman Major .

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Imbir, G. et al. (2021). Shockwave-Generating Interdisciplinary Methods Used to Elaborate Acellular Tissue Origin Extracellular Matrix. In: Gzik, M., Paszenda, Z., Pietka, E., Tkacz, E., Milewski, K. (eds) Innovations in Biomedical Engineering. AAB 2020. Advances in Intelligent Systems and Computing, vol 1223. Springer, Cham. https://doi.org/10.1007/978-3-030-52180-6_32

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