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Chemical Isotope Labeling LC-MS for Metabolomics

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Cancer Metabolomics

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 1280))

Abstract

Due to the great diversity of chemical and physical properties of metabolites as well as a wide range of concentrations of metabolites present in metabolomic samples, performing comprehensive and quantitative metabolome analysis is a major analytical challenge. Conventional approach of combining various techniques and methods with each detecting a fraction of the metabolome can lead to the increase in overall metabolomic coverage. However, this approach requires extensive investment in equipment and analytical expertise with still relatively low coverage and low sample throughput. Chemical isotope labeling (CIL) liquid chromatography mass spectrometry (LC-MS) offers an alternative means of increasing metabolomic coverage while maintaining high quantification precision and accuracy. This chapter describes the CIL LC-MS method and its key features for metabolomic analysis.

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Authors and Affiliations

  1. Department of Chemistry, University of Alberta, Edmonton, AB, Canada

    Shuang Zhao & Liang Li

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  1. Shuang Zhao
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  2. Liang Li
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Correspondence to Liang Li .

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  1. University of California, Los Angeles, Los Angeles, CA, USA

    Shen Hu

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Zhao, S., Li, L. (2021). Chemical Isotope Labeling LC-MS for Metabolomics. In: Hu, S. (eds) Cancer Metabolomics. Advances in Experimental Medicine and Biology, vol 1280. Springer, Cham. https://doi.org/10.1007/978-3-030-51652-9_1

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