Abstract
Intensive use of pesticides results in environmental pollution and food contamination. Use of microbial elicitors, enhancing plant immunity, is a promising alternative to fungicidal treatments. MF3, an elicitor protein from Pseudomonas fluorescens, has a strong protective action toward a range of plant pathogens, but can be degraded by UV radiation and plant proteases under field conditions. MF3 encapsulation in biodegradable poly-3-hydroxybutyrate (PHB) protects it against the adverse external factors. Using a model plant-pathogen system, we demonstrated that the treatment of wheat leaf fragments with a MF3-PHB complex prior artificial inoculation with Parastagonospora nodorum, a major wheat pathogen, provides a higher suppression of the infection (41.5%) than the treatment with MF3 alone (28.9%) or PHB (2.5%). Thus, encapsulating this protein elicitor may provide prolonged and more efficient activity under field conditions and is able to promote the development of MF3-based biopesticides as an efficient alternative to commercial fungicides.
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References
Maffei, M.E., Arimura, G.I., Mithoefer, A.: Natural elicitors, effectors and modulators of plant responses. Nat. Prod. Rep. 29, 1288–1303 (2012)
Dodds, P.N., Rathjen, J.P.: Plant immunity: towards an integrated view of plant-pathogen interactions. Nat. Rev. Genet. 11, 539–548 (2010)
Shumilina, D., Kramer, R., Klocke, E., Dzhavakhiya, V.: MF3 (peptidyl-prolyl cis-trans isomerase of FKBP type from Pseudomonas fluorescens)—an elicitor of non-specific plant resistance against pathogens. Phytopathol. Pol. 41, 39–49 (2006)
Neves-Petersen, M.T., Gajula, G.P., Petersen, S.B.: UV light effects on proteins: from photochemistry to nanomedicine. In: Molecular photochemistry—various aspects, pp. 125–161. InTechOpen, Rijeka (2012).
Thomas, E.L., van der Hoorn, R.A.L.: Ten prominent host proteases in plant-pathogen interactions. Int J Mol Sci. 19(2), 639 (2018)
Popletaeva, S.B., Voinova, T.M., Kartashov, M.I., Pasechnik, T.D., Arslanova, L.R., Shcherbakova, L.A., Statsyuk, N.V., Bonartsev, A.P., Zernov, A.L., Bonartseva, G.A., Dzhavakhiya, V.G.: Biodegradable poly-3-hydroxybutyrate as a shielding carrier for a plant-protecting MF3 protein from Pseudomonas fluorescens. Biointerface Res. Appl. Chem. 8(3), 3224–3231 (2018)
Popletaeva, S.B., Statsyuk, N.V., Voinova, T.M., Arslanova, L.R., Zernov, A.L., Bonartsev, A.P., Bonartseva, G.A., Dzhavakhiya, V.G.: Evaluation of eliciting activity of peptidyl prolyl cys/trans isomerase from Pseudomonas fluorescens encapsulated in sodium alginate regarding plant resistance to viral and fungal pathogens. AIMS Microbiol. 4(1), 192–208 (2018)
Bonartsev, A., Yakovlev, S., Boskhomdzhiev, A., Zharkova, I., Bagrov, D., Myshkina, V., Mahina, T., Kharitonova, E., Samsonova, O., Zernov, A., Zhuikov, V., Efremov, Y., Voinova, V., Bonartseva, G., Shaitan, K.: The terpolymer produced by Azotobacter chroococcum 7B: effect of surface properties on cell attachment. PLoS ONE 8(2), e57200 (2013)
Acknowledgements
The study was carried out within the framework of the state assignment of the Ministry of Science and Higher Education of the Russian Federation (theme no. 0598-2019-0002). P. nodorum strain B-24/MC2 was provided by the State Collection of Plant Pathogenic Microorganisms, Indicator Plants, and Differential Cultivars of the All-Russian Research Institute of Phytopathology.
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Voinova, T., Kartashov, M., Shcherbakova, L., Statsyuk, N., Dzhavakhiya, V. (2021). MF3 Protein Encapsulation in Biodegradable Poly-3-Hydroxybutyrate Improves Its Protective Action Against a Major Wheat Pathogen Parastagonospora Nodorum. In: Ksibi, M., et al. Recent Advances in Environmental Science from the Euro-Mediterranean and Surrounding Regions (2nd Edition). EMCEI 2019. Environmental Science and Engineering(). Springer, Cham. https://doi.org/10.1007/978-3-030-51210-1_218
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DOI: https://doi.org/10.1007/978-3-030-51210-1_218
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