Abstract
Microalgae have been recognized as the foundation of the food chain in aquatic ecosystems and one of the most prominent characteristics of algae is their color, which is determined by their pigments, hence, microalgal biomass is attracting worldwide attention. These pigments are colorful chemical substances that are part of the photosynthetic system of microalgae and are distinguished into three classes: carotenoids, chlorophylls, and phycobiliproteins. Besides the color, pigments have health-promoting properties and a broad range of potential industrial applications. Consumers are becoming increasingly aware of the correlation between diet, health, and disease prevention. Despite the beneficial properties of pigments provided by microalgae, their effectiveness at preventing a range of diseases depends on their bioaccessibility and bioavailability. The digestion process comprises several steps, which promotes an intense variation of the conditions that the pigments are exposed, and therefore, could in several ways compromises the health benefits caused by microalgae pigment consumption. Therefore, the present chapter aims to present the main methods used to assess the bioaccessibility and bioavailability of pigments from microalgae to better understand the processes involved. Consequently, providing information about the most accepted analytical protocols in measurement of bioavailability of pigments.
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References
Asai, A., Yonekura, L., & Nagao, A. (2008). Low bioavailability of dietary epoxyxanthophylls in humans. British Journal of Nutrition, 100, 273–277.
Begum, H., Yusoff, F. M. D., Banerjee, S., Khatoon, H., & Shariff, M. (2016). Availability and utilization of pigments from microalgae. Critical Reviews in Food Science and Nutrition, 56, 2209–2222.
Brown, E. M., Nitecki, S., Pereira-Caro, G., McDougall, G. J., Stewart, D., Rowland, I., et al. (2014). Comparison of in vivo and in vitro digestion on polyphenol composition in lingonberries: Potential impact on colonic health. BioFactors, 406, 611–623.
Cha, K. H., Koo, S. Y., Song, D. G., & Pan, C. H. (2012). Effect of microfluidization on bioaccessibility of carotenoids from Chlorella ellipsoidea during simulated digestion. Journal of Agricultural and Food Chemistry, 60, 9437–9442.
Chernomorsky, S., Segelman, A., & Poretz, R. D. (1999). Effect of dietary chlorophyll derivatives on mutagenesis and tumor cell growth. Teratogenesis, Carcinogenesis, and Mutagenesis, 19, 313–322.
Egger, L., Ménard, O., Delgado-Andrade, C., Alvito, P., Assunção, R., Balance, S., et al. (2016). The harmonized INFOGEST in vitro digestion method: From knowledge to action. Food Research International, 88, 217–225.
Ferruzzi, M. G., Failla, M. L., & Schwartz, S. J. (2001). Assessment of degradation and intestinal cell uptake of carotenoids and chlorophyll derivatives from spinach puree using an in vitro digestion and caco-2 human cell model. Journal of Agricultural and Food Chemistry, 49, 2082–2089.
Ferruzzi, M. G., Failla, M. L., & Schwartz, S. J. (2002). Sodium copper chlorophyllin: In vitro digestive stability and accumulation by caco-2 human intestinal cells. Journal of Agricultural and Food Chemistry, 50, 2173–2179.
Gille, A., Hollenbach, R., Trautmann, A., Posten, C., & Briviba, K. (2019). Effect of sonication on bioaccessibility and cellular uptake of carotenoids from preparations of photoautotrophic Phaeodactylum tricornutum. Food Research International, 118, 40–48.
Gille, A., Neumann, U., Louis, S., Bischoff, S. C., & Briviba, K. (2018). Microalgae as a potential source of carotenoids: Comparative results of an in vitro digestion method and a feeding experiment with C57BL/6J mice. Journal of Functional Foods, 49, 285–294.
Gille, A., Trautmann, A., Posten, C., & Briviba, K. (2016). Bioaccessibility of carotenoids from Chlorella vulgaris and Chlamydomonas reinhardtii. International Journal of Food Sciences and Nutrition, 67, 507–513.
Granado-Lorencio, F., Herrero-Barbudo, C., Acién-Fernández, G., Molina-Grima, E., Fernández-Sevilla, J., Pérez-Sacristán, B., & Blanco-Navarro, I. (2009). In vitro bioaccesibility of lutein and zeaxanthin from the microalgae Scenedesmus almeriensis. Food Chemistry, 114, 747–752.
Guedes, A. C., Amaro, H. M., & Malcata, F. X. (2011). Microalgae as sources of carotenoids. Marine Drugs, 9, 625–644.
Hartmann, D., Thürmann, P. A., Spitzer, V., Schalch, W., Manner, B., & Cohn, W. (2004). Plasma kinetics of zeaxanthin and 3′-dehydro-lutein after multiple oral doses of synthetic zeaxanthin. American Journal of Clinical Nutrition, 79, 410–417.
Mackie, A., & Rigby, N. (2015). InfoGest consensus method. In K. Verhoeckx, P. Cotter, I. López-Expósito, C. Kleiveland, T. Lea, A. Mackie, et al. (Eds.), The impact of food bioactives on health: In vitro and ex vivo models (pp. 13–22). New York: Springer.
Minic, S. L., Stanic-Vucinic, D., Mihailovic, J., Krstic, M., Nikolic, M. R., & Cirkovic Velickovic, T. (2016). Digestion by pepsin releases biologically active chromopeptides from C-phycocyanin, a blue-colored biliprotein of microalga Spirulina. Journal of Proteomics, 147, 132–139.
Ranga Rao, A., Baskaran, V., Sarada, R., & Ravishankar, G. A. (2013). In vivo bioavailability and antioxidant activity of carotenoids from microalgal biomass—A repeated dose study. Food Research International, 54, 711–717.
Ranga Rao, A., Raghunath Reddy, R. L., Baskaran, V., Sarada, R., & Ravishankar, G. A. (2010). Characterization of microalgal carotenoids by mass spectrometry and their bioavailability and antioxidant properties elucidated in rat model. Journal of Agricultural and Food Chemistry, 58, 8553–8559.
Rao, V. G., Banerjee, C., Ghosh, S., Mandal, S., Kuchlyan, J., & Sarkar, N. (2013). A step toward the development of high-temperature stable ionic liquid-in-oil microemulsions containing double-chain anionic surface active ionic liquid. The Journal of Physical Chemistry B, 117, 7472–7480.
Sangeetha, R. K., Bhaskar, N., Divakar, S., & Baskaran, V. (2009). Bioavailability and metabolism of fucoxanthin in rats: Structural characterization of metabolites by LC-MS (APCI). Molecular and Cellular Biochemistry, 333, 299.
Shibata, S., & Hayakawa, K. (2009). Bioavailability of lutein in Chlorella powder: A single ingestion of Chlorella powder raises serum lutein concentrations in healthy human volunteers. Food Science and Technology Research, 15, 449–452.
Sugawara, T., Baskaran, V., Tsuzuki, W., & Nagao, A. (2002). Brown algae fucoxanthin is hydrolyzed to fucoxanthinol during absorption by caco-2 human intestinal cells and mice. Journal of Nutrition, 132, 946–951.
Wu, Q., Fu, X. P., Zhang, Q., Liu, G. M., Cao, M. J., & Cai, Q. F. (2015). Effects of physicochemical factors and in vitro gastrointestinal digestion on antioxidant activity of R-phycoerythrin from red algae Bangia fusco-purpurea. International Journal of Food Sciences and Technology, 50, 1445–1451.
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Braga, A.R.C., de Rosso, V.V. (2020). Analytical Protocols in the Measurement of Pigments’ Bioavailability. In: Jacob-Lopes, E., Queiroz, M., Zepka, L. (eds) Pigments from Microalgae Handbook. Springer, Cham. https://doi.org/10.1007/978-3-030-50971-2_10
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DOI: https://doi.org/10.1007/978-3-030-50971-2_10
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