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Phytohormones and Growth Regulators

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Plant Cell and Tissue Culture – A Tool in Biotechnology

Abstract

Phytohormones are defined as substances produced in some tissues at certain developmental stages of a plant. They occupy a central position in the regulation of growth, and especially of differentiation of plants in general, as well as in cell and tissue culture systems. On the basis of such reactions, phytohormones are usually divided into five groups, i.e., auxins, gibberellins, cytokinins, some gaseous compounds like ethylene, and a group associated predominantly with growth retardation and senescence, such as abscisic acid (ABA), and based on more recent data, Jasmonic acid and Brassinosteroids, and also salicylic acid. The significance of growth regulators applied with the nutrient medium has been dealt here. Endogenous hormonal system of carrot cell cultures in the NL medium have been determined. Twenty-four hours after application of 14C-labeled IAA (side chain labeled), the isotope could be detected in several fractions of the tissue, as well as in the nutrient solution. Interaction of IAA and ethylene is discussed. Finally, some remarks on hormone autotrophic cultures have been made.

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References

  • Bai B, Su YH, Yuan J, Zhang XS (2013) Induction of somatic embryos in Arabidopsis requires local YUCCA expression mediated by the down-regulation of ethylene biosynthesis. Mol Plant 6:1247–1260

    Article  CAS  Google Scholar 

  • Bender L, Neumann K-H (1978) Investigations on Indole-3-acetic acid metabolism of carrot tissue cultures. Z Pflanzenphysiol 88:209–217

    Article  CAS  Google Scholar 

  • Blakesley D, Lanten JR, Morgan R (1986) The metabolism of 6-benzylaminopurine and Zeatin in Gerbera shoots. In: Somers DA, Gengenbach DG, Biesboer DD, Hackett WP, Green CE (eds) Abstr. 6th Int. congress plant tissue and cell culture. University of Minnesota, Minneapolis, MN, p 375

    Google Scholar 

  • Cheng ZJ, Wang L, Sun W, Zhang Y, Zhou C, Su YH, Li W, Sun T, Zhao XY, Li XG, Cheng Y, Zhao Y, Xie Q, Zhang XS (2013) Pattern of auxin and cytokinin responses for shoot meristem induction results from the regulation of cytokinin biosynthesis by auxin response factor 3. Plant Physiol 161:240–251

    Article  CAS  Google Scholar 

  • Coumans-Gilles MF, Kefers C, Coumans M, Gaspar T (1982) Auxin content and metabolism in auxin repairing and -non- requiring cultures. In: Fujiwara A (ed) Plant tissue culture. Japanese Association for Plant Tissue Culture, Tokyo, pp 197–198

    Google Scholar 

  • Croes AF, Barendse GWM (1986) Uptake and metabolism of hormones as related to in vitro flower bud development in tobacco. In: Somers DA, Gengenbach DG, Biesboer DD, Hackett WP, Green CE (eds) Abstr. 6th Int. congress plant tissue and cell culture. University of Minnesota, Minneapolis, MN, p 299

    Google Scholar 

  • Ermoshin AA, Kiseleva IS, Bortsova SA, Sanaeva YV, Alekseeva VV (2016) Morphological features of the transgenic tobacco plant shoot expressing the 3-hydroxy-3-methylglutagyl-CoA reductase (HMG1) gene in the direct and reverse orientations towards the promoter. Russ J Dev Biol 47(4):216–222

    Article  CAS  Google Scholar 

  • Fukuda H (1997) Tracheary element differentiation. Plant Cell 9:1147–1156

    Article  CAS  Google Scholar 

  • Ghosh S, Pal A (2013) Proteomic analysis of cotyledonary explants during shoot organogenesis in Vigna radiata. Plant Cell Tissue Organ Cult 115:55–68. https://doi.org/10.1007/s11240-013-0340-0

    Article  CAS  Google Scholar 

  • Ghosh S, Pal A (2014) Abscisic acid, one of the key determinants of in vitro shoot differentiation from cotyledons of Vigna radiata. Am J Plant Sci 5:704–713

    Article  Google Scholar 

  • Hasen CE, Meins F, Milani A (1985) Clonal and physiological variation in cytokinin content of tobacco cell lines differing in cytokinin requirements and capacity for neoplastic growth. Differentiation 29:1–6

    Article  Google Scholar 

  • Hasen CE, Meins F, Aebi R (1987) Hormonal regulation of zeatin ribosid accumulation by cultured tobacco cells. Planta 172:520–525

    Article  Google Scholar 

  • Houba-Herin N, Perthe C, d’Alayer J, Laloue M (1999) Cytokinin oxidase from Zea mays: purification C DNA cloning and expression in moss protoplast. Plant J 17:615–626

    Article  CAS  Google Scholar 

  • Jones B, Gunnerås SA, Petersson SV, Tarkowski P, Graham N, May S, Dolezal K, Sandberg G, Ljung K (2010) Cytokinin regulation of auxin synthesis in Arabidopsis involves a homeostatic feedback loop regulated via auxin and cytokinin signal transduction. Plant Cell 22:2956–2969

    Article  CAS  Google Scholar 

  • Kakimoto T (2001) Identification of plant cytokinin biosynthetic enzymes as dimethylallyldiphosphate: ATP/ADP isopentenyl transferases. Plant Cell Physiol 42:677–685

    Article  CAS  Google Scholar 

  • Kohli A, Sreenivasulu N, Lakshmanan P, Kumar PP (2013) The phytohormone crosstalk paradigm takes center stage in understanding how plants respond to abiotic stresses. Plant Cell Rep 32:945–957. https://doi.org/10.1007/s00299-013-1461-y

    Article  CAS  PubMed  Google Scholar 

  • Li T, Neumann K-H (1985) Embryogenesis and endogenous hormone content of cell cultures of some carrot varieties (Daucus carota L.). Ber Dtsch Bot Ges 98:227–235

    Google Scholar 

  • Maiti S, Chakraborty D, Kundu S, Paul S, Sengupta S, Pal A (2011) Developmentally regulated temporal expression and differential acid invertase activity in differentiating cotyledonary explants of mungbean [Vigna radiata (L.) Wilczek]. Plant Cell Tissue Organ Cult 107:417–425

    Article  CAS  Google Scholar 

  • Morris RO, Bilyeu KD, Laskey LG, Cheikh NN (1999) Isolation of a gene encoding a glycosylated cytokinin oxidase from maize. Biochem Biophys Res Commun 255:328–333

    Article  CAS  Google Scholar 

  • Ohashi-Ito K, Oguchi M, Kojima M, Sakakibara H, Fukuda H (2013) Auxin-associated initiation of vascular cell differentiation by LONESOME HIGHWAY. Development 140:765–769

    Article  CAS  Google Scholar 

  • Pernisova M, Klima P, Horak J, Válková M, Malbeck J, Souček P, Reichman P, Hoyerová K, Dubová J, Friml J, Zažímalová E, Hejátko J (2009) Cytokininsmodulate auxin-induced organogenesis in plants via regulation of the auxin efflux. Proc Natl Acad Sci U S A 106:3609–3614

    Article  CAS  Google Scholar 

  • Rajasekaran K, Heim MB, Davis GC, Carnes MG, Vasil IK (1987) Endogenous plant growth regulators in leaves and tissue cultures of Napier grass (Pennisetum purpureum Schum.). J Plant Physiol 130:13–25

    Article  CAS  Google Scholar 

  • Scarpella E, Helariutta Y (2010) Vascular pattern formation in plants. Curr Top Dev Biol 91:221–265

    Article  CAS  Google Scholar 

  • Seldimirova OA, Kudoyarova GR, Kruglova NN, Zaytsev DY, Veselov SY (2016) Changes in distribution of zeatin and indole-3-acetic acid in cells during callus induction and organogenesis in vitro in immature embryo culture of wheat. In Vitro Cell Dev Biol Plant 52:251–264

    Article  CAS  Google Scholar 

  • Sengupta S, Das S, Ghosh S, Pal A (2011) Phenolic signals and its perception leads to in vitro shoot induction in Vigna radiata cotyledonary explants. Int J Plant Dev Biol 5(1):37–41

    Google Scholar 

  • Stiebeling B, Neumann K-H (1987) Identification and concentration of cytokinins in carrots (Daucus carota L.) as influenced by development and a circardian rhythem. J Plant Physiol 127:111–121

    Article  CAS  Google Scholar 

  • Takei K, Sakakiwara H, Sugiyama T (2001) Identification of genes encoding adenylate isopentenyl transferases, a cytokinin biosynthesis enzyme in Arabidopsis thaliana. J Biol Chem 276:26405–26410

    Article  CAS  Google Scholar 

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Neumann, KH., Kumar, A., Imani, J. (2020). Phytohormones and Growth Regulators. In: Plant Cell and Tissue Culture – A Tool in Biotechnology. Springer, Cham. https://doi.org/10.1007/978-3-030-49098-0_11

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