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Clinical Probes for ROS and Oxidative Stress

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Measuring Oxidants and Oxidative Stress in Biological Systems

Part of the book series: Biological Magnetic Resonance ((BIMR,volume 34))

Abstract

Electron paramagnetic resonance (EPR) spectroscopy is considered as a valuable tool in the determination and characterization of free radicals in vitro and in animal models; however, its use in humans presents technical challenges. While spin traps and spin probes have their own advantages and disadvantages, there are several factors that need to be considered for the appropriateness of any possible applications, which is a topic that will be discussed in this chapter. Besides the use of exogenous probes for the detection of free radicals, several endogenous molecules are used to determine the redox status in patients using the EPR techniques. In this chapter, both endogenous and exogenous agents for clinical studies of oxidative stress will be discussed. EPR signal formation or disappearance is a measure of the extent of ROS production, but changes in the spectral profile are also exploited, such as in the case of some trityl radical probes. The mechanisms of signal formation, disappearance, or changes thereof will be mentioned in detail in this chapter along with the limitations in their applications and cautionary notes in their interpretation.

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Authors and Affiliations

  1. Department of Biological Chemistry and Pharmacology, The Ohio State University, Columbus, OH, USA

    Pedro L. Zamora & Frederick A. Villamena

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  1. Pedro L. Zamora
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  2. Frederick A. Villamena
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  1. Department of Chemistry and Biochemistry, University of Denver, Centennial, CO, USA

    Lawrence J. Berliner

  2. Department of Internal Medicine, Division of Pulmonary, Critical Care, and Sleep Medicine, Ohio State University College of Medicine, Dorothy M. Davis Heart and Lung Research Institute, Columbus, OH, USA

    Narasimham L. Parinandi

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Zamora, P.L., Villamena, F.A. (2020). Clinical Probes for ROS and Oxidative Stress. In: Berliner, L., Parinandi, N. (eds) Measuring Oxidants and Oxidative Stress in Biological Systems. Biological Magnetic Resonance, vol 34. Springer, Cham. https://doi.org/10.1007/978-3-030-47318-1_3

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