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Improvement of Onion Through Accelerated Approaches

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Accelerated Plant Breeding, Volume 2

Abstract

The bulb onion (Allium cepa L.) is grown on all continents except Antarctica and is prized by essentially all of the world’s cultures for its flavour and health-enhancing attributes. Both open-pollinated (OP) and hybrid cultivars are commonly grown, with hybrids predominating in most regions of the world. Hybrids are produced using cytoplasmic male sterility (CMS), and different sources of CMS are commercially used. Onion breeders focus primarily on bulb characteristics such as colour, shape, soluble solid content, pungency and flavour, storage ability and health-enhancing attributes, as well as plant characters such as resistances to diseases, pests and bolting. Important characteristics for seed production include uniform flowering, straight seed stalks, stable expression of male sterility and seed yield. Most of these traits show moderate to high heritability and therefore respond to selection. Due to the biennial generation time of the onion, the development of value-added populations and hybrids is a time-consuming and expensive process. The use of biotechnological approaches, such as marker-aided selection, production of doubled haploids, gene editing and cytoplasmic conversions, offers great promise for population improvement and hybrid development addressing changes in consumer preference and production environments.

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References

  • Alan AR, Brants A, Cobb ED, Goldschmied PA, Mutschler MA, Earle ED (2004) Fecund gynogenic lines from onion (Allium cepa L.) breeding materials. Plant Sci 167:1055–1066

    Google Scholar 

  • Alan AR, Lim W, Mutschler MA, Earle ED (2007) Complementary strategies for ploidy manipulations in gynogenic onion (Allium cepa L.). Plant Sci 173:25–31

    Google Scholar 

  • Anandhan S, Chavan AA, Gopal J, Mote SR, Shelke PV, Lawande KE (2014) Variation in gynogenic potential for haploid induction in Indian shortday onions. Ind J Genet 74(4):526–528

    Google Scholar 

  • Asakaviciute R (2008) Androgenesis in anther culture of Lithuanian spring barley (Hordeum vulgare L.) and potato (Solanum tuberosum L.) cultivars. Turk J Biol 32:155–160

    Google Scholar 

  • Baek G, Kim CW, Kim S (2017) Development of a molecular marker tightly linked to the C locus conferring a white bulb color in onion (Allium cepa L.) using bulked segregant analysis and RNA-Seq. Mol Breed 37:94

    Google Scholar 

  • Bahorun T, Luximon RA, Crozier A, Aruoma OI (2004) Total phenol, flavonoid, proanthocyanidin and vitamin C levels and antioxidant activities of Mauritian vegetables. J Sci Food Agric 84:1553–1561

    CAS  Google Scholar 

  • Bang H, Cho DY, Yoo KS, Yoon MK, Patil BS, Kim S (2011) Development of simple PCR-based markers linked to Ms locus, a restorer-of-fertility gene in onion (Allium cepa L.). Euphytica 179:439–449

    Google Scholar 

  • Başay S, Ellialtıoğlu SS (2013) Effect of genotypical factors on the effectiveness of anther culture in eggplant (Solanum melongena L.). Turk J Biol 37:499–505

    Google Scholar 

  • Bhat JG, Murthy HN (2007) Factors affecting in vitro gynogenic haploid production in Niger (Guizotia abyssinica (L. f.) Cass.). Plant Growth Regul 52:241–248

    Google Scholar 

  • Blackslee AF, Belling J, Farnham ME, Bergner AD (1922) A haploid mutant in the jimson weed, Datura stramonium. Science 55:646–647

    Google Scholar 

  • Bohanec B (2002) Doubled haploid onions. In: Rabinowitch H, Currah L (eds) Allium crop science: recent advances. CABI Publishing, Wallingford, pp 145–148

    Google Scholar 

  • Bohanec B, Jakse M (1999) Variations in gynogenic response among long-day onion (Allium cepa L.) accessions. Plant Cell Rep 18:737–742

    Google Scholar 

  • Bohanec B, Jakse M, Ihan A, Javornik B (1995) Studies of gynogenesis in onion (Allium cepa L.): induction procedures and genetic analysis of regenerants. Plant Sci 104:215–224

    Google Scholar 

  • Bohanec B, Jakse M, Havey MJ (2003) Genetic analyses of gynogenetic haploid production in onion. J Am Soc Hortic Sci 128:571–574

    Google Scholar 

  • Brewster JL (2008) Onions and other vegetable alliums. CABI, Oxfordshire

    Google Scholar 

  • Burk LG, Gerstel DU, Wernsman EA (1979) Maternal haploids of Nicotiana tabacum L. from seed. Science 206:585

    Google Scholar 

  • Campion B, Alloni C (1990) Induction of haploid plants in onion (Allium cepa L.) by in vitro culture of unpollinated ovules. Plant Cell Tissue Organ Cult 20:1–6

    Google Scholar 

  • Campion B, Azzimonti MT (1988) Evolution of ploidy level in haploid plants of onion (Allium cepa L.) obtained through in vitro gynogenesis. In: Eucarpia 4th Allium symposium, Institute of Horticultural Research, Warwick, pp 85–89

    Google Scholar 

  • Campion B, Falavigna A, Schiavi M (1984) Efforts for in vitro androgenesis in onion (Allium cepa L.). In: Proceedings of 3rd allium symposium eucarpia, p 110

    Google Scholar 

  • Campion B, Azzimonti MT, Vicini E, Schiayi M, Falavigna A (1992) Advances in haploid induction in onion (Allium cepa L.) through in vitro gynogenesis. Plant Sci 86:97–104

    Google Scholar 

  • Campion B, Perri E, Azzimonti MT, Vicini E, Schiavi M (1995) Spontaneous and induced chromosome doubling in gynogenic lines of onion (Allium cepa L.). Plant Breed 114:243–246

    Google Scholar 

  • Chen JF, Cui L, Malik AA, Mbira KG (2010) In vitro haploid and dihaploid production via unfertilized ovule culture. Plant Cell Tissue Organ Cult 104:311–319

    Google Scholar 

  • Cho K, Yang TJ, Hong SY, Kwon YS, Woo JG, Park HG (2006) Determination of cytoplasmic male sterile factors in onion plants (Allium cepa L.) using PCR-RFLP and SNP markers. Mol Cell 21(3):411–417

    Google Scholar 

  • Chu YH, Chang CL, Hsu HF (2000) Flavonoid content of several vegetables and their antioxidant activity. J Sci Food Agr 80:561–566

    CAS  Google Scholar 

  • Clarke AE, Jones HA, Little TM (1944) Inheritance of bulb color in the onion. Genetics 29:569–575

    CAS  PubMed  PubMed Central  Google Scholar 

  • D’Angelo CJ, Goldman IL (2018) Temporal aspects of Vernalization and flowering in long-day storage onion. J Am Soc Hortic Sci 143(6):446–453

    Google Scholar 

  • Darbyshire B, Henry RJ (1979) The association of fructans with high percentage dry matter weight in onion cultivars suitable for dehydrating. J Sci Food Agric 30:1035–1038

    Google Scholar 

  • D’Angelo CJ, Goldman IL (2019) Breaking onion bulb Endodormancy with hydrogen peroxide. Hortic Sci 54(10):1694–1702

    Google Scholar 

  • de Courcel AG, Veder F, Boussac JM (1989) DNA polymorphism in Allium cepa cytoplasms and its implications concerning the origin of onions. Theor Appl Genet 77:793

    Google Scholar 

  • Dhatt AS (2016) Development of technologies for increasing productivity and availability of onion. In: Chadha KL et al (eds) doubling farmers income through horticulture. Astral International Pvt. Ltd., New Delhi, pp 154–163

    Google Scholar 

  • Dhatt AS (2019) Potential and constraints of F1 hybrids in tropical onion. In: Proceedings: international symposium on edible alliums: challenges and opportunities held during Feb 9–12 at YASHADA, Pune, pp 48–57

    Google Scholar 

  • Donner H, Gao L, Mazza G (1997) Separation and characterization of simple malonylated anthocyanins in red onions Allium cepa L. Food Res Int 30:637–647

    Google Scholar 

  • Duangjit J, Welsh K, Wise M, Bohanec B, Havey MJ (2014) Genetic analyses of anthocyanin concentrations and intensity of red bulb color among segregating haploid progenies of onion. Mol Breed 34(1):75–85

    CAS  Google Scholar 

  • Ebrahimi R, Zamani Z (2009) Effect of polyamines on in vitro Gynogenesis of onion (Allium cepa L.). Am Eurasian J Sustain Agric 3:71–74

    Google Scholar 

  • El-Shafie MW, Davis GN (1967) Inheritance of bulb color in the onion (Allium cepa L.). Hilgardia 38(17):607–622

    Google Scholar 

  • Engelke T, Terefe D, Tatlioglu T (2003) A PCR-based marker system monitoring CMS (S), (T) and N cytoplasm in the onion (Allium cepa L). Theor Appl Genet 107:162–167

    Google Scholar 

  • Fayos O, Valles MP, Garces-Claver A, Mallor C, Castillo AM (2015) Doubled haploid production from Spanish onion (Allium cepa L.) germplasm: embryogenesis induction, plant regeneration and chromosome doubling. Front Plant Sci 6:1–11

    Google Scholar 

  • Ficcadenti N, Sestili S, Annibali S (1999) In vitro gynogenesis to induce haploid plants in melon (Cucumis melo L.). J Genet Breed 53:255–257

    Google Scholar 

  • Forster BP, Thomas WTB (2005) Doubled haploids in genetics and plant breeding. Plant Breed Rev 25:57–88

    CAS  Google Scholar 

  • Forster BP, Heberle-Bors E, Kasha KJ, Touraev A (2007) The resurgence of haploids in higher plants. Trends Plant Sci 12:368–375

    CAS  Google Scholar 

  • Fossen T, Andersen OM, Ovstedal DO, Pedersen AT, Raknes A (1996) Characteristic anthocyanin pattern from onions and other Allium spp. J Food Sci 61:703–706

    Google Scholar 

  • Fuleki A (1971) Anthocy anins in red onion, Allium cepa. J Food Sci 36(1):101–104

    Google Scholar 

  • Galazka J, Niemirowicz-Szczytt K (2013) Review of research on haploid production in cucumber and other cucurbits. Folia Hortic 25:67–78

    Google Scholar 

  • Galmarini CR, Goldman IL, Havey MJ (2001) Genetic analyses of correlated solids, flavor, and health-enhancing traits in onion (Allium cepa L.). Mol Gen Genomics 265:543–551

    Google Scholar 

  • Gemes JA, Venczel G, Balogh P (1997) Haploid plant induction in zucchini (Cucurbita pepo L. convar. Giromontiina DUCH) and in cucumber (Cucumis sativus L.) lines through in vitro gynogenesis. Acta Hortic 447:623–624

    Google Scholar 

  • Geoffriau E, Kahane R, Rancillac M (1997) Variation of gynogenesis ability in onion (Allium cepa L.). Euphytica 94:37–44

    Google Scholar 

  • Germana MA (2010) Anther culture for haploid and doubled haploid production. Plant Cell Tissue Organ Cult 104:283–300

    Google Scholar 

  • Gökçe AF, Havey MJ (2002) Linkage equilibrium among tightly linked RFLPs and the Ms locus in open‐pollinated onion populations. J Am Soc Hortic Sci 127:944–946

    Google Scholar 

  • Grzebelus E, Adamus A (2004) Effect of anti-mitotic agents on development and genome doubling of gynogenic onion (Allium cepa L.) embryos. Plant Sci 167:569–574

    Google Scholar 

  • Guha S, Johri BM (1966) In vitro development of ovary and ovule of Allium cepa L. Phytomorphology 16:353–364

    Google Scholar 

  • Guha S, Maheshwari SC (1964) In vitro production of embryos from anthers of Datura. Nature 204:497

    Google Scholar 

  • Gurel S, Gurel E, Kaya Z (2000) Doubled haploid plant production from unpollinated ovules of sugar beet (Beta vulgaris L.). Plant Cell Rep 19:1155–1159

    Google Scholar 

  • Havey MJ (1993) A putative donor of S-cytoplasm and its distribution among open-pollinated populations of onion. Theor Appl Genet 86:128–134

    CAS  PubMed  Google Scholar 

  • Havey MJ (1995) Identification of cytoplasms using the polymerase chain reaction to aid in the extraction of maintainer lines from open-pollinated populations of onion. Theor Appl Genet 90:263–268

    CAS  PubMed  Google Scholar 

  • Havey MJ (2013) Single nucleotide polymorphisms in linkage disequilibrium with the male-fertility restoration (Ms) locus in onion. J Am Soc Hortic Sci 119:90–93

    Google Scholar 

  • Hermsen JG, Ramana MA (1981) Haploidy and plant breeding. Philos Trans R Soc Lond B 292:499–507

    Google Scholar 

  • Holford P, Croft JH, Newbury HJ (1991) Differences between and possible origins of the cytoplasms found in fertile and male sterile onions (Allium cepa L). Theor Appl Genet 82:737–744

    Google Scholar 

  • Huo YM, Liu BJ, Yang YY, Miao J, Gao LM, Kong SP, Wang ZB, Kitano H, Wu X (2015) AcSKP1, a multiplex PCR-based co-dominant marker in complete linkage disequilibrium with the male-fertility restoration (Ms) locus, and its application in open-pollinated populations of onion. Euphytica 204:711–722

    Google Scholar 

  • Jakse M, Bohanec B, Ihan A (1996) Effect of media components on the gynogenic regeneration of onion (Allium cepa L.) cultivars and analysis of regenerants. Plant Cell Rep 15:934–938

    Google Scholar 

  • Jakse M, Bohanec B, Havey MJ (2003) Chromosome doubling procedures of onion (Allium cepa L.) gynogenic embryos. Plant Cell Rep 21:905–910

    Google Scholar 

  • Jakse M, Hirschegger P, Bohanec B, Havey MJ (2010) Evaluation of gynogenic responsiveness and pollen viability of selfed doubled haploid onion lines and chromosome doubling via somatic regeneration. J Am Soc Hortic Sci 135:67–73

    Google Scholar 

  • Jauhar PP, Xu SS, Baenziger PS (2009) Haploidy in cultivated wheats: induction and utility in basic and applied research. Crop Sci 49:737–755

    Google Scholar 

  • Javornik B, Bohanec B, Campion B (1998) Studies in the induction of a second cycle gynogenesis in onion (Allium cepa L.) and genetic analysis of the plant. Plant Breed 117:275–278

    Google Scholar 

  • Jones HA, Clarke AE (1943) Inheritance of male sterility in onion and the production of hybrid seed. Proc Am Soc Hortic Sci 43:189–194

    Google Scholar 

  • Jones HA, Emsweller SL (1936) A male sterile onion. Proc Am Soc Hortic Sci 34:582–585

    Google Scholar 

  • Kadams AM, Nwasike CC (1986) Heritability and correlation studies of some vegetative traits in Nigerian local white onion. Plant Breed 97:232–236

    Google Scholar 

  • Kasha KJ, Kao KN (1970) High frequency haploid production in barley (Hordeum vulgare L.). Nature 225:874–876

    Google Scholar 

  • Keller ERJ (1990) Culture of unpollinated ovules, ovaries and flower buds in some species of the genus Allium and haploid induction via gynogenesis in onion (Allium cepa L.). Euphytica 47:241–247

    Google Scholar 

  • Khar A, Jakse J, Havey MJ (2008) Segregations for onion bulb colors reveal that red is controlled by at least three loci. J Am Soc Hortic Sci 133(1):42–47

    CAS  Google Scholar 

  • Khar A, Saini N (2016) Limitations of PCR-based molecular markers to identify male-sterile and maintainer plants from Indian onion (Allium cepa L.) populations. Plant Breeding 135(4):519–524

    Google Scholar 

  • Khosa JS, Mc Callum J, Dhatt AS, Macknight RC (2016) Enhancing onion breeding using molecular tools. Plant Breed 135:9–20

    Google Scholar 

  • Khush GS, Virmani SS (1996) Haploids in plant breeding. In: Jain SM, Sopory SK, Veilleux RE (eds) In vitro haploid production in higher plants, vol 1. Kluwer Academic Publisher, Dordrecht, pp 11–33

    Google Scholar 

  • Kim S (2014) A codominant molecular marker in linkage disequilibrium with a restorer-of-fertility gene (Ms) and its application in reevaluation of inheritance of fertility restoration in onions. Mol Breed 34:769–778

    CAS  Google Scholar 

  • Kim B, Kim S (2019) Identification of a variant of CMS-T cytoplasm and development of high resolution melting markers for distinguishing cytoplasm types and genotyping a restorer-of-fertility locus in onion (Allium cepa L.). Euphytica 215:164

    Google Scholar 

  • Kim S, Binzel M, Yoo K, Park S, Pike LM (2004a) Pink (P). A new locus responsible for a pink trait in onions (Allium cepa) resulting from natural mutations of Anthocyanidin synthase. Mol Gen Genomics 272:18–27

    Google Scholar 

  • Kim S, Binzel M, Yoo K, Park S, Pike LM (2004b) Inactivation of DFR (Dihydroflavonol 4-reductase) gene transcription results in blockage of anthocyanin production in yellow onions (Allium cepa). Mol Breed 14:253–263

    Google Scholar 

  • Kim S, Yoo K, Pike LM (2005a) Development of a co-dominant PCR-based marker for an allelic selection of the pink trait in onions (Allium cepa) based on the insertion mutation in the promoter of the Anthocyanidin synthase gene. Theor Appl Genet 110:628–633

    Google Scholar 

  • Kim S, Yoo K, Pike LM (2005b) Development of a PCR-based marker utilizing a deletion mutation in the DFR (Dihydroflavonol 4-reductase) gene responsible for the lack of anthocyanin production in yellow onions (Allium cepa). Theor Appl Genet 110:588–595

    Google Scholar 

  • Kim S, Bang H, Yoo KS, Pike LM (2006) Identification of the fourth allele of the ANS (Anthocyanidin synthase) gene and its effect on red color intensity in onions (Allium cepa). Euphytica 149:45–51

    Google Scholar 

  • Kim S, Lee ET, Cho DY, Han T, Bang H, Patil BS, Ahn YK, Yoon MK (2009) Identification of a novel chimeric gene, orf725, and its use in development of a molecular marker for distinguishing among three cytoplasm types in onion (Allium cepa L.). Theor Appl Genet 118:433–441

    Google Scholar 

  • Kim S, Yoon M (2010) Comparison of mitochondrial and chloroplast genome seg‑ ments from three onion (Allium cepa L.) cytoplasm types and identification of a transsplicing intron of cox2. Curr Genet 56:177–188

    Google Scholar 

  • Kim S, Kim CW, Park M, Choi D (2015) Identification of candidate genes associated with fertility restoration of cytoplasmic male-sterility in onion (Allium cepa L.) using a combination of bulked segregant analysis and RNA-seq. Theor Appl Genet 128(11):2289–2299

    Google Scholar 

  • King JJ, Bradeen JM, Havey MJ (1998) Variability for RFLPs and relationships among elite commercial inbred an virtual hybrid populations of onion. J Am Soc Hortic Sci 123:1034–1037

    CAS  Google Scholar 

  • Kobayashi RS, Sinden SL, Bouwkamp JC (1993) Ovule cultures of sweet potato (Ipomoea batatas) and closely related species. Plant Cell Tissue Organ Cult 32:77–82

    Google Scholar 

  • Kohn V, Kielkowska A, Havey MJ (2013) Sequencing and annotation of the chloroplast DNAs of normal(N) male fertile and male sterile (S) cytoplasms of onion and single nucleotide polymorphisms distinguishing these cytoplasms. Genome 56:737–742

    Google Scholar 

  • Kumar NKK, Gopal J, Parthasarthy (2015) The Onion. ICAR, New Delhi, India

    Google Scholar 

  • Lilly JW, Havey MJ (2001) Sequence analysis of a chloroplast intergenic spacer for phylogenetic estimates in Allium section Cepa and a PCR-based polymorphism detecting mixtures of male-fertile and male-sterile cytoplasmic onion. Theor Appl Genet 102:78–82

    Google Scholar 

  • Lin M, Watson JF, Baggett JR (1995) Inheritance of soluble solids and pyruvic acid content of bulb onion. J Am Soc Hortic Sci 120:119–122

    CAS  Google Scholar 

  • Liu S, Yeh C, Tang HM, Nettleton D, Schnable PS (2012) Gene mapping via bulked segregant RNA-Seq (BSR-Seq). PLoS One 7:e36406

    CAS  PubMed  PubMed Central  Google Scholar 

  • Maraschin SF, Priester W, Spaink HP, Wang M (2005) Androgenic switch: an example of plant embryogenesis from the male gametophyte perspective. J Exp Bot 56:1711–1726

    CAS  PubMed  Google Scholar 

  • Martinez LE, Augero CB, Lopez ME, Galmarini CR (2000) Improvement of in vitro gynogenesis induction in onion (Allium cepa L.) using polyamines. Plant Sci 156:221–226

    Google Scholar 

  • Masuzaki S, Shigyo M, Yamauchi N (2006) Direct comparison between genomic constitution and flavonoid contents in Allium multiple alien addition lines reveals chromosomal locations of genes related to biosynthesis from dihydrokaempferol to quercetin glucosides in scaly leaf of shallot (Allium cepa L.). Theor Appl Genet 112:607–617

    Google Scholar 

  • McCallum J, Clarke A, Pither-Joyce M, Shaw M, Butler R, Brash D, Scheffer SI, Heusden S, Shigyo M, Havey MJ (2006) Genetic mapping of a major gene affecting onion bulb fructan content. Theor Appl Genet 112:958–967

    CAS  PubMed  Google Scholar 

  • McCallum J, Pither-Joyce M, Shaw M, Kenel F, Davis S, Butler R, Scheffer J, Jakse J, Havey MJ (2007) Genetic mapping of sulfur assimilation genes reveals a QTL for onion bulb pungency. Theor Appl Genet 114:815–822

    CAS  PubMed  Google Scholar 

  • McCollum GD (1968) Heritability and genetic correlation of soluble solids, bulb size and shape in while sweet Spanish onion. Can J Genet Cylol 10:508–514

    Google Scholar 

  • Michalik B, Adamus A, Nowak E (2000) Gynogenesis in polish onion cultivars. J Plant Physiol 156:211–216

    CAS  Google Scholar 

  • Michelmore RW, Paran I, Kesseli RV (1991) Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA 88:9828–9832

    CAS  PubMed  Google Scholar 

  • Moqbeli E, Peyvast G, Hamidoghli Y, Olfati JA (2013) In vitro cucumber haploid line generation in several new cultivars. J Mol Biol Biotechnol 21:18–25

    Google Scholar 

  • Muren RC (1989) Haploid plant induction from unpollinated ovaries in onion. Hortic Sci 24:833–834

    Google Scholar 

  • Musial K, Bohanec B, Przywara L (2001) Embryological study on gynogenesis in onion (Allium cepa L.). Sex Plant Reprod 13:335–341

    Google Scholar 

  • Musial K, Bohanec B, Jakse M, Przywara L (2005) The development of onion (Allium cepa L.) embryo sacs in vitro and gynogenesis induction in relation to flower size. Vitro Cell Dev Biol Plant 41:446–452

    Google Scholar 

  • Nitsch C (1977) Culture of isolate microspore. In: Reinert J, Bajaj YPS (eds) Applied and fundamental aspects of plant cell, tissue and organ culture. Springer, Berlin, pp 268–278

    Google Scholar 

  • Olszewska D, Kisiala A, Niklas-nowak A, Nowaczyk P (2014) Study of in vitro anther culture in selected genotypes of genus Capsicum. Turk J Biol 38:118–124

    Google Scholar 

  • Ponce M, Martinez L, Galmarini CR (2006) Influence of CCC, putrescine and gellam gum concentration on gynogenic embryo induction in Allium cepa. Biol Plant 50:425–428

    CAS  Google Scholar 

  • Puddephat IJ, Robinson HT, Smith BM, Lynn J (1999) Influence of stock plant pretreatment on gynogenic embryo induction from flower buds of onion. Plant Cell Tissue Organ Cult 57:145–148

    Google Scholar 

  • Raines S, Henson C, Havey MJ (2009) Genetic analyses of soluble carbohydrate concentrations in onion bulbs. J Am Soc Hortic Sci 134:618–623

    Google Scholar 

  • Reiman GH (1931) Genetic factors for pigmentation in the onion and their relationship to disease resistance. J Agric Res 42:251–278

    Google Scholar 

  • Reinert J, Bajaj YPS (1977) Anther culture: haploid production and its significance. In: Reinert J, Bajaj YPS (eds) Applied and fundamental aspects of plant cell, tissue and organ culture. Springer, Berlin, pp 251–267

    Google Scholar 

  • Rhodes MJC, Price KR (1996) Analytical problems in the study of flavonoid compounds in onions. Food Chem 57:113–117

    CAS  Google Scholar 

  • Ritsema T, Smeekens S (2003) Fructans: beneficial for plants and humans. Curr Opin Plant Biol 6:223–230

    Google Scholar 

  • Sato Y (1998) PCR amplification of CMS-specific mitochondrial nucleotide sequences to identify cytoplasmic genotypes of onion (Allium cepa L.) sequences. Theor Appl Genet 96:367–370

    Google Scholar 

  • Sato Y, Nagai M, Mikami T, Kinoshita T (1993) The use of mitochondrial DNA polymorphism in the classification of individual onion plants by cytoplasmic genotypes. Theor Appl Genet 86:345–348

    Google Scholar 

  • Schweisguth B (1973) Etude d’un nouveau type de sterilite male chez l’oignon, Allium cepa L. Ann Amelior Plant 23:221–233

    Google Scholar 

  • Shalaby TA (2007) Factors affecting haploid induction through in vitro gynogenesis in summer squash (Cucurbita pepo L.). Scientia Hortic 115:1–6

    Google Scholar 

  • Simon PW (1995) Genetic analysis and total soluble solids in long storage onion. Euphytica 82:1–8

    CAS  Google Scholar 

  • Terahara N, Yamaguch MA, Honda T (1994) Malonylated anthocyanins from bulbs of red onion Allium cepa L. Biosci Biotechnol Biochem 58:1324–1325

    Google Scholar 

  • Thakur P (2016) In vitro production of double haploids (DH) in onion (Allium cepa L.). PhD Thesis, Punjab Agricultural University Ludhiana

    Google Scholar 

  • Thomas WTB, Newton AC, Wilson A, Booth A, Macaulay M, Keith R (2000) Development of recombinant chromosome substitution lines: a barley resource. SCRI Annual Report, pp 99–100

    Google Scholar 

  • Tsushida T, Suzuki M (1995) Isolation of flavonoid-glycosides in onion and identification by chemical synthesis of the glycosides. J Jpn Soc Food Sci Technol 42:100–108

    CAS  Google Scholar 

  • Watson A, Ghosh S, Williams MJ, Cuddy WS et al (2018) Speed breeding is a powerful tool to accelerate crop research and breeding. Nat Plants 4(1):23–29

    PubMed  Google Scholar 

  • Winzeler H, Schmid J, Fried PM (1987) Field performance of androgenetic doubled haploid spring wheat line in comparison with line selected by the pedigree system. Plant Breed 99:41–48

    Google Scholar 

  • Yang HY, Zhou C (1982) In vitro induction of haploid plants from unpollinated ovaries and ovules. Theor Appl Genet 63:97–104

    CAS  PubMed  Google Scholar 

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Khosa, J.S., Dhatt, A.S. (2020). Improvement of Onion Through Accelerated Approaches. In: Gosal, S., Wani, S. (eds) Accelerated Plant Breeding, Volume 2. Springer, Cham. https://doi.org/10.1007/978-3-030-47298-6_3

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