Abstract
Marine litter is one of the most important human-caused pressures on the marine environment, it is defined as “any persistent, manufactured or processed solid material discarded, disposed of or abandoned in the marine and coastal environment” [1, 2]. The most abundance part of marine litter is composed by plastic [3]. The main and most remarkable risks for the species inhabiting the marine environment are entanglement and accidental ingestion. Moreover, from the direct release of particles of plastic and as a fragmentation of large items consequence, originate microplastic. The presence of microplastics in the marine environment has raised scientific interest during the last decade. As demonstrated, ingestion of marine litter can have lethal and sub-lethal effects on wildlife that accidentally ingests it, and sea turtles are particularly susceptible. For this reason loggerhead turtle (C. caretta) has been identified by the European Commission (EC), in the Marine Strategy Framework Directive (MSFD) [4, 5], as indicator for monitoring the amount of Marine Litter ingested by marine animals in Mediterranean basin. The Istituto Zooprofilattico Sperimentale dell’Abruzzo e Molise “G. Caporale” (IZSAM) is contributing to monitor the impact of marine litter on sea turtles and biota for MSFD, thanks to the participation of EC funded-project “Implementation Of Indicators Of Marine Litter On Sea Turtles And Biota In Regional Sea Conventions And Marine Strategy Framework Directive Areas – INDICIT” (https://indicit-europa.eu/). This is an European program to implement novel protocols and indicators of litter impact on marine turtles [6] Data from Adriatic coast are provided by IZSAM that is a member of Abruzzo and Molise stranding network instituted by Regional Government [7, 8], whose main objectives is diagnostic investigation of dead cause of stranded marine animals with the aim conservation of protected species, the indirect control of environmental status and the protection of public health.
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References
UNEP: Marine Litter: An Analytical Overview. UNEP, Nairobi (2005)
UNEP: Marine Litter: A Global Challenge. UNEP, Nairobi (2009)
Pham, C.K., Ramirez-Llodra, E., Alt, C.H.S., Amaro, T., Bergmann, M., Canals, M., Company, J.B., Davies, J., Duineveld, G., Galgani, F., Howell, K.L., Huvenne, V.A.I., Isidro, E., Jones, D.O.B., Lastras, G., Morato, T., Gomes-Pereira, J.N., Purser, A., Stewart, H., Tojeira, I., Tubau, X., Van Rooij, D., Tyler, P.A.: Marine litter distribution and density in european seas, from the shelves to deep basins. PLoS ONE 9(4), 1–13 (2014)
MSFD-TSGML: Guidance on monitoring of marine litter in European Seas. A guidance document within the common implementation strategy for the marine strategy framework directive. EUR-26113 EN. JRC Scientific and Policy Reports JRC83985 (2013). http://mcc.jrc.ec.europa.eu/documents/201702074014.pdf
Matiddi, M., van Franeker, J.A., Sammarini, V., Travaglini, A., Alcaro, L.: Monitoring litter by sea turtles: an experimental protocol in the Mediterranean. In: Proceedings of the 4th Mediterranean Conference on Sea Turtles, 7–10 November, Naples (2011)
Matiddi, M., Hochsheid, S., Baini, M., Cocumelli, C., Serena, F., Travaglini, A., Marra, S., Campani, T., Scholl, F., Mancusi, C., Amato, E., Brigugli, P., Maffucci, F., Fossi, M.C., Bentivegna, F., Andrea de Lucia, G.: Loggerhead sea turtles (Caretta caretta): a target species for monitoring litter ingested by marine organisms in the Mediterranean Sea. Environ. Pollut. 230, 199–209 (2017)
Determinazione 31/12/2014, n DG21/167. Applicazione nella Regione Abruzzo del Regolamento (CE) n. 1069/2009 recante: “norme sanitarie relative ai sottoprodotti di origine animale non destinati al consumo umano”. Approvazione Linee Guida sulle procedure per lo smaltimento degli animali morti incluso il regolamento di attuazione per gli animali marini spiaggiati
DCA 16/02/2018 n.12. Recepimento dell’Accordo tra il Governo, le Regioni e le Province Autonome di Trento e di Bolzano sullo schema di linee guida per il recupero, soccorso, affidamento e gestione delle tartarughe marine ai fini della riabilitazione e per la manipolazione a scopi scientifici (Rep Atti n. 83 CSR del 10 luglio 2014); Procedura Regionale per la Gestione delle segnalazioni di carcasse di tartarughe marine o di cetacei ritrovate sul litorale molisano, e del ritrovamento di animali marini vivi, feriti e/o in difficoltà
Avio, C.G., Gorbi, S., Regoli, F.: Experimental development of a new protocol for extraction and characterization of microplastics in fish tissues: first observations in commercial species from Adriatic. Mar. Environ. Res. 111, 18–26 (2015)
Foekema, E.M., De Gruijter, C., Mergia, M.T., van Franeker, J.A., Murk, AT.J., Koelmans, A.A.: Plastic in north sea fish. Environ. Sci. Tecnol. 47(15), 8818–8824 (2013)
Matiddi, M., deLucia, G.A., Silvestri, C., Darmon, G., Tomás, J., Pham, K.C., Camedda, A., Vandeperre, F., Claro, F., Kaska, J., Kaberi, H., Revuelta, O., Piermarini, R., Daffina, R., Pisapia, M., Genta, D., Sözbilen, D., Bradai, M.N., Rodríguez, J., Gambaiani, D., Tsangaris, C., Chaieb, O., Moussier, J., Loza, A.L., Miaud C.: Data collection on marine litter ingestion in sea turtles and thresholds for good environmental status. JoVE. https://www.jove.com/video/59466/data-collection-on-marine-litter-ingestion-sea-turtles-thresholds-for (2019)
Cózar, A., Echevarría, J.F., González-Gordillo, I., Irigoien, X., Úbeda, B., Hernández-León, S., Palma, A.T., Navarro, S., García-de-Lomas, J., Ruiz, A., Fernández-de-Puelles, M.L., Duarte, C.M.: Plastic debris in the open Ocean. PNAS 111(28), 10239–10244 (2014)
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Di Renzo, L. et al. (2020). Extraction Protocol Optimization for Detection of Microplastics in Digestive System Contents of Loggerhead Turtle (Caretta Caretta). In: Cocca, M., et al. Proceedings of the 2nd International Conference on Microplastic Pollution in the Mediterranean Sea. ICMPMS 2019. Springer Water. Springer, Cham. https://doi.org/10.1007/978-3-030-45909-3_33
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