Abstract
Rubber tree is an important crop species grown over an expansive area in southern Thailand to harvest natural latex. The most commonly grown clone here is RRIM 600, but other clones are also grown in some areas, including BPM 24 which is a cytoplasmic male sterile elite clone. The organellar genomes, from both mitochondria and chloroplast of BPM 24 have been analyzed. The chloroplast sequence of BPM 24 is nearly identical to that published for the clone RRIM 600 with the exception of 5 SNPs, which were either non-coding or silent. The mitochondrial genome is remarkably different from that of other rubber tree clones such as RRIM 600, and the cause of cytoplasmic sterility has been identified as a novel transcript containing a portion of ATPase subunit 9 (atp9). The nuclear genome of BPM 24 was assembled using a combination of Illumina and PacBio sequence data and Chicago HiC scaffolding, which produced an assembly containing a relatively small number of fragmented contigs. A high-density SNP map was used to anchor almost one-third of the sequence into 18 linkage groups that captured two-thirds of the protein coding sequences. Comparing orthologous genes with other members of the Euphorbiaceae family identified cassava as the closest relative with a shared whole genome duplication event that took place following a split from a common ancestor with castor bean.
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Shearman, J.R., Pootakham, W., Tangphatsornruang, S. (2020). The BPM 24 Rubber Tree Genome, Organellar Genomes and Synteny Within the Family Euphorbiaceae. In: Matsui, M., Chow, KS. (eds) The Rubber Tree Genome. Compendium of Plant Genomes. Springer, Cham. https://doi.org/10.1007/978-3-030-42258-5_4
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DOI: https://doi.org/10.1007/978-3-030-42258-5_4
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