Abstract
The presence of aflatoxin M1 (AFM1) in raw milk should be continuously monitored to protect the population from the risks associated with proven toxicity and carcinogenicity. The ELISA method offers many advantages, including shorter time of analysis, simultaneous analysis of a large number of samples, acceptable as a screening method, limited use of organic solvents, absence of complicated sample preparation steps and simplicity of analytical procedure compared to long-lasting and expensive chromatographic techniques. The test is highly sensitive with a detection limit of 0.01 μg/kg and a quantitative range of 0.01–0.1 μg/kg. In the Una-Sana Canton in 2018, 27 samples of raw cow milk were analysed and all the analyses were carried out twice. The highest concentration of AFM1 was in December and it was 0.032 μg/kg, which is a permissible concentration of AFM1 according to the Regulations on Maximum levels for certain contaminants in food, Official Gazette No. 37/09, which is in compliance with European Union regulations (EU) and the Codex Alimentarius Commission (CAC). In a case of a positive result, the samples are analysed several times and sent to a certified chemical laboratory for the super analysis for the confirmation of the results.
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Jukić, H., Dedić, S., Rodić, M., Jusufhodžić, Z., Demirović, D. (2020). Determination of Aflatoxin M1 in Raw Milk by the ELISA Method in the Una-Sana Canton. In: Brka, M., Omanović-Mikličanin, E., Karić, L., Falan, V., Toroman, A. (eds) 30th Scientific-Experts Conference of Agriculture and Food Industry. AgriConf 2019. IFMBE Proceedings, vol 78. Springer, Cham. https://doi.org/10.1007/978-3-030-40049-1_33
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